Anti-profilin: rabbit polyclonal

Anti-profilin: rabbit polyclonal

Product Uses
The following protocols have been tested with this antibody:

Western BlotImmunocytochemistryELISAImmunoprecipitation




Profilin antibody is provided as an affinity purified rabbit polyclonal antibody. The antibody has been raised against purified human profilin and it is predicted to have broad species cross reactivity. The positive control protein consists of an extract of bovine thymus that identifies a characteristic profilin band at 14 kDa. The control protein (bovine thymus extract, Cat. # EXT03) is supplied as a lyophilized powder; this should be reconstituted in 500 μl of cold Milli-Q water to 2 mg/ml. After addition of water the protein should be left at room temperature for 5 minutes with occasional pipetting up and down to make sure that the protein has completely dissolved into solution, it can then be stored at -20°C. We recommend running 10 μg of positive control protein in Western blots.


The profilin antibody (50 μg) is supplied as a lyophilized powder. It is recommended to resuspend the powder in 200 μl of 30% glycerol/water. This gives a concentration of 250 μg/ml in PBS plus 30% glycerol with 0.01% chlorhexidine for preservative.


Figure 1: Western blot probed with profilin antibody. Lane 1; 100 ng profilin protein, Lane 2; 10 μg thymus extract protein, Lane 3; 10 μg of platelet extract protein. Primary antibody used at 1:1000 dilution, secondary antibody used at 1/50 000 dilution anti-rabbit HRP, Supersignal West Dura™ (Pierce Co.) detection method with film exposure for 30 seconds.

For product Datasheets and MSDSs please click on the PDF links below.   For additional information, click on the FAQs tab above or contact our Technical Support department at

AuthorTitleJournalYearArticle Link
Romero, Ana M. et al.Chronic alcohol alters dendritic spine development in neurons in primary cultureNeurotoxicity research2013ISSN 1476--3524
Osiak, Anna Eleonor et al.Subconfluent endothelial cells form podosomes downstream of cytokine and RhoGTPase signalingExperimental cell research2005ISSN 0014--4827
Hernández-Deviez, Delia J. et al.ARNO and ARF6 regulate axonal elongation and branching through downstream activation of phosphatidylinositol 4-phosphate 5-kinase alphaMolecular biology of the cell2004ISSN 1059--1524
Innocenti, Metello et al.Abi1 is essential for the formation and activation of a WAVE2 signalling complexNature cell biology2004ISSN 1465--7392
Tang, Dale D. et al.Role of Crk-Associated Substrate in the Regulation of Vascular Smooth Muscle ContractionHypertension2003ISSN 1524-4563
Tang, Dale D. et al.Downregulation of profilin with antisense oligodeoxynucleotides inhibits force development during stimulation of smooth muscleAmerican journal of physiology. Heart and circulatory physiology2003ISSN 0363--6135


Question 1: What is the specificity of the profilin antibody?

Answer 1:  The profilin antibody (Cat. # APUF01) will specifically react with profilin of mouse, rat, and human origin, there are no other bands on the western blots. 


Question 2: What epitopes on profilin does this antibody recognize?

Answer 2: The profilin antibody (Cat. # APUF01) is a polyclonal antibody and so recognizes multiple epitopes on human profilin as it was raised against the entire purified human profilin protein. 



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