Recently, Zhan et al. investigated the molecular pathways underlying human epidermal stem cell (hESC) migration during wound repair to better understand the activity of these cells in the restoration of skin cell and hair follicle homeostasis during wound repair and healing. The authors discovered that nitric oxide (NO) stimulates hESC migration during wound repair and healing, and using the NO donor S-nitroso-N-acetylpenicillamine (SNAP), demonstrated that NO promotes the migration of hESCs in vivo and in vitro. Furthermore, NO-mediated hESC migration in vitro requires cGMP-mediated activation of RhoA and Rac1 (but not Cdc42) signaling pathways. These signaling pathways were examined given that cell migration requires dynamic cell morphology changes which necessitate the re-arrangement of a cell’s actin cytoskeleton, which is strongly regulated by Rho-family GTPases. Cytoskeleton’s Cdc42, Rac1, and RhoA pull-down activation assays (Cat. # BK034, BK035, BK036, respectively) were essential in this study, providing researchers with the necessary research tools to measure Rho-family GTPase activities in a sensitive and consistent manner. These results provide valuable insight into the essential role that Rho-family GTPases have in NO-mediated hESC migration during wound repair and healing to restore proper cellular homeostasis.