Cytoskeleton, Inc. has been a reliable source of protein purification services in the areas of recombinant and native source proteins. We emphasize high purity and biological activity which enables the proteins to be used in many applications including diagnostic kits, pre-clinical drug development programs, early compound screening in primary HTS projects, and in ELISA formats. We also have extensive experience in gene design and expression with an eye to produce the correct orientation and solubility for particular applications. Many purifications use the unique skills and expertise in cytoskeletal and signal transductions proteins. Using this combined knowldge we have produced many functional assays for kinesins (e.g. Eg5, CenPE, MKLP2), myosins (e.g. cardiac, smooth, skeletal and non-muscle isoforms, sarcomere assays), small G-proteins (e.g. Rho, Arf, Ral families), tubulins (e.g. tumor, plant and fungal origins), tubulin binding proteins (e.g. tau and MAPs) and actin binding proteins (e.g. cofilin, profiling, gelsolin), many of them are multi-protein assays that might have protein complexes of 3 or more subunits e.g. a soluble sarcomere format and the Arp2/3 complex based assay. We have generated a set of defined modules that allow one to chose the desired package for your application, see below for module details. Other experience in antibody and ELISA technology and protein synthesis complements the cytoskeletal and signal transduction focus. We support all of our services with a dedicated technical services department and years of laboratory experience in the fields of cell biology, cancer biology, cardio-vascular research and neuroscience.
For more details in protein purification services click on Protein Purification
If you have an immediate question please send an e-mail describing your requirements to tservice@cytoskeleton.com
Examples of protein purification are:
1. Cancer cell line tubulins are useful for developing drugs targeted toward cancer cells. We have isolated 1.0-5.0 mg of 90% pure tubulin from 50 g of cells.
2. Small G-protein Arf1 and Arf6 within 8 weeks to >85% purity, and suitable for 10,000 GEF assays (50 x 1mg).
We perform full quality control, which includes:
1. Full quantitation of yield, in mg per g tissue.
2. PAGE-SDS of purified proteins, stained with coomassie blue and scanned with a densitometer, to estimate purity.
3. Biological activity determination depending on the requirements of the customer.
For more information click here.
Protein purification modules:
Type | Name | Example Protein | Amount | Mod. # | More information |
Recombinant protein | Small protein or protein domain (<30kDal) with gene provided by customer. | RhoA small G-protein | 10 x 100 µg | REC012 | |
Recombinant protein | Small protein or protein domain (<30kDal) with gene synthesis. | RhoA small G-protein | 10 x 100 µg | REC022 | |
Recombinant protein | Medium to large protein or protein domain (31-100 kDal) with gene synthesis. | CenPE kinesin | 10 x 100 µg | REC032 | |
Recombinant protein | Small protein or protein domain (<30kDal) with gene synthesis and expression in eukaryotic system. | RhoA small G-protein with lipid tail | 10 x 100 µg | REC041 | |
Recombinant protein | Medium to large protein or protein domain (31-100 kDal) with gene synthesis and expression in eukaryotic system. | Recombinant beta-Tubulin | 10 x 100 µg | REC042 | |
Recombinant protein | Medium to large protein or protein domain (31-100 kDal) with multiple gene synthesis and co-expression in eukaryotic system. (useful for protein complex production). | Recombinant alpha/beta- Tubulin heterodimer | 10 x 100 µg | REC043 | |
Native source protein | Cited protein purification | Arp2/3 complex (seven protein complex) | 10 x 100 µg | REC052 |