Answer 1: Kinesin motor proteins use microtubules (MTs) as a substrate to orchestrate a wide range of kinetic events within a cell. Kinesins operate by utilizing the energy of ATP by hydrolysis, an activity that is greatly enhanced in the presence of MTs. Microtubule activation of the ATPase activity of motor proteins can be measured with a kinesin ATPase assay. Cytoskeleton developed two such assays, one end-point (Cat. # BK053) and one kinetic (Cat. # BK060), that are used to measure inorganic phosphate (Pi) levels generated by MT-activated kinesin adenosine triphosphatase (ATPase) activity. These two kinesin ATPase biochem assay kits contain MTs and kinesin heavy chain (KHC) protein along with the necessary buffers and reagents to measure Pi production as a means of measuring molecular motor activity. These kits are useful for discovering kinesin inhibitors and activators (Cat. # BK053 and BK060) as well as determining Vmax and Kcat values for a kinesin motor protein (Cat. # BK060).
Cytoskeleton, Inc. also offers a Biochem Kit (Cat. # BK027) which allows the visualization of motor protein motility using fluorescently-labeled microtubules.
Answer 2: Yes, Cytoskeleton, Inc. has an active contract services department that performs custom purifications to produce purified recombinant proteins with high biological activity. Examples of protein purification projects are:
We perform full quality control, which includes:
The delivery time usually depends on the protein or type of assay, but 4 to 8 weeks is normal.
The cost depends on the quantity and quality of the protein required, and also on whether the preparation is simple or complex. The normal range is $8,000 to $12,000. However, there are circumstances where we may consider a lower cost if we can introduce the protein into our range of products. There is an initial deposit of 50% of the total cost which is non-refundable. The remaining 50% is due 30 days after the product has been delivered.
Please send an e-mail describing your requirements to firstname.lastname@example.org and provide the following information to expedite the creation of a quotation: