Fluorescent Actin

Fluorescent Actin Proteins

Cytoskeleton Inc. offers several fluorescently-labeled purified actin proteins including the new HiLyte™488 actin and HiLyte™555 actins.  The expanded offering will provide more versatility for researchers while complementing our existing labeled actin tools that are used in an array of applications including labeling F-actin barbed ends with rhodamine actin.   See the Application Table below for a variety of in vivo & in vitro uses for these reagents. 

Applications

  Application Reference
Modeling in vitro bio membranes 1, 2
Molecular Mechanisms underlying skeletal mediated force/stress  3, 4, 5, 6
in vitro modeling of the cytoskeleton in the cell cortex   7
Study mechanisms of in vivo actin dynamics by labeling of free barbed ends of actin filaments  8, 9, 10, 11
Study actin binding proteins  12, 13, 14
Applications in functional nanodevices  15, 16

F-Actin Barbed End Labeling (Cat # AR05)

Adapted From: Actin at stereocilia tips is regulated by mechanotransduction and ADF/cofilin. 2021. McGrath J. et al. Curr. Bio. 31:1141-1153

Method Collection - Video Examples

  1. Actin protein (rhodamine): human platelet APHR
    Actin protein (rhodamine): human platelet
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  2. Actin protein (rhodamine): rabbit skeletal muscle AR05
    Actin protein (rhodamine): rabbit skeletal muscle
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  3. Actin: HiLyte™ Fluor 488 Labeled (Rabbit skeletal muscle, >99% pure) AR06

    Actin: HiLyteFluor™ 488 Labeled (Rabbit skeletal muscle, >99% pure)

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  4. Actin: HiLyte™ Fluor 555 Labeled (Rabbit skeletal muscle, >99% pure) AR07

    Actin: HiLyteFluor™ 555 Labeled (Rabbit skeletal muscle, >99% pure)

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