Figure 2. Absorbance and excitation spectra of MemGlow NR12S diluted in DMSO to 4.6 µM. Absorbance peak detected at 550 nm with peak emission at 633 nm.
MemGlow NR12S is a solvatochromic photostable plasma membrane-targeting dye. Upon binding with a plasma membrane in a predominant liquid ordered (LO) phase NR12S exhibits a 45-50 nm wavelength shift relative to liquid disordered (LD) phase enabling investigators to examine the nanoscale distribution of local chemical polarity in plasma membranes. NR12S can also be used to label the plasma membrane for conventional fluorescence microscopy imaging.
NR12S Membrane Polarity Probe Product Functions (MG08)
MemGlow NR12S is a solvatochromic photostable plasma membrane-targeting dye
Examine the nanoscale distribution of local chemical polarity in plasma membranes
NR12S can also be used to label the plasma membrane for conventional fluorescence microscopy imaging.
For more detailed information on using this product see the About Tab
** 4 nmol of MemGlow will produce a 20µM stock solution when reconstituted with 200 µl of anhydrous DMSO
Figure 3. Widefield fluorescent imaging of live HeLa S1 cells labeled with 10 nM NR12S. HeLa S1 cells were imaged with a TRITC filter set, neutral density filter, a digital CCD camera, and 100x oil objective (false colored red).
The absorption max of NR12S is 554 nm, with an emission spectra of 635 nm, an extinction coefficient of 45,000 M-1.cm-1 , and can be visualized using a Cy 3.5 filter set or other suitable filter sets. NR12S is supplied as a lyophilized pellet. NR12S is a lipid binding dye and appropriate PPE should be worn at all times. Dispose of NR12S according to local regulations and policies.
Storage and Reconstitution
The lyophilized product is stable at 4°C (<10% humidity) for 6 months and should be protected from light. To reconstitute, briefly centrifuge to collect the product at the bottom of the tube. NR12S should be reconstituted with 200 µl of anhydrous DMSO to create a 20 µM stock solution for cell imaging. After reconstitution the solution should be stored at -20°C where it is stable for 3 months. Once reconstituted, allow product to warm to room temperature before opening tube.
Important Technical Notes
Table 1. Recommended initial concentrations. Optimal conditions for efficient labeling should be determined for each cell line and application.
MEMBRIGHT™ is a trademark of CNRS/UNISTRA of France.