Live Cell Imaging Reagents

Cytoskeleton Inc. - Fluorescent Proteins and Reagents

Cytoskeleton offers several reagents for live-cell research including fluorescent actin, ECM, and tubulin proteins, as well as, cell permeable protein activators and inhibitors. This includes our new HiLyte™488 actin and HiLyte™555 actin tools that provide more versatility for researchers while complementing our existing labeled actin and tubulin tools that are used in an array of applications. 

Spirochrome Probes For Bioimaging

The SiR- , SPY-, PK-, and Flipper-TR Live-cell imaging probes developed by Spirochrome are cell-permeable compounds which label microtubules, F-actin , Lysosomes, membrane, mitochondria, and chromosomal DNA in living cells. SPY™ probes improve upon the SiR live cell imaging technology while also expanding the fluorophore labeling options for the study of F-actin, microtubules, and DNA in living cells.  

MemGlow™ Probes For Bioimaging: From The MemBright Family Of Probes

Cytoskeleton Inc. offers an array of innovative probes to visualize the cellular plasma membrane, which was originally developed by the makers of the MemBright probes. MemGlow™ probes provide high photostability, exhibit excellent fluorescent quantum yields, and some of them are super-resolution compatible.  Also available are NR Polarity Probes which are solvatochromic photostable plasma membrane-targeting dyes.

HiLyte™555 labeled actin

SiR Actin Labeled Cells

NR12A Membrane Probe

Cytoskeleton's live cell imaging products have been cited hundreds of times over the past two decades. A select few are described here. More individual product citations listed on each product page.

AuthorTitleJournalYearArticle Link
De Abreu, Isadora Rocha et al.A molecular analysis of substituted phenylethylamines as potential microtubule targeting agents through in silico methods and in vitro microtubule-polymerization activityScientific Reports 2023 13:12023ISSN 2045--2322
Singh, Rajendra K. et al.Coordinated Biophysical Stimulation of MSCs via Electromagnetized Au-Nanofiber Matrix Regulates Cytoskeletal-to-Nuclear Mechanoresponses and Lineage SpecificationAdvanced Functional Materials2023ISSN 1616--3028
Castrogiovanni, Cédric et al.Evidence for a HURP/EB free mixed-nucleotide zone in kinetochore-microtubulesNature Communications 2022 13:12022ISSN 2041--1723
Masucci, Erin M. et al.Microtubule dynamics influence the retrograde biased motility of kinesin-4 motor teams in neuronal dendritesMolecular Biology of the Cell2022ISSN 1939-4586
Henty-Ridilla, Jessica L.Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence (TIRF) MicroscopyJoVE (Journal of Visualized Experiments)2022ISSN 1940--087X
Sasanpour, Mehrzad et al.Reconstituting and Characterizing Actin-Microtubule Composites with Tunable Motor-Driven Dynamics and MechanicsJoVE (Journal of Visualized Experiments)2022ISSN 1940--087X
Baron, Desiree M et al.ALS-associated KIF5A mutations abolish autoinhibition resulting in a toxic gain of function.Cell reports2022ISSN 2211--1247
Cheng, Xianrui et al.Xenopus laevis egg extract preparation and live imaging methods for visualizing dynamic cytoplasmic organizationJournal of Visualized Experiments2021ISSN 1940-087X
Goldstein-Levitin, Alina et al.Intracellular functions and motile properties of bi-directional kinesin-5 cin8 are regulated by neck linker dockingeLife2021ISSN 2050-084X
Lee, Gloria et al.Active cytoskeletal composites display emergent tunable contractility and restructuringSoft Matter2021ISSN 1744-6848
Aher, Amol et al.CLASP Mediates Microtubule Repair by Restricting Lattice Damage and Regulating Tubulin IncorporationCurrent Biology2020ISSN 1879-0445
Nolet, Felix E. et al.Nuclei determine the spatial origin of mitotic waveseLife2020ISSN 2050-084X
Chen, Keyu et al.Giant ankyrin-B suppresses stochastic collateral axon branching through direct interaction with microtubulesJournal of Cell Biology2020ISSN 1540-8140
Rodríguez-García, Ruddi et al.Mechanisms of Motor-Independent Membrane Remodeling Driven by Dynamic MicrotubulesCurrent Biology2020ISSN 1879-0445
Farhadi, Leila et al.Actin and microtubule crosslinkers tune mobility and control co-localization in a composite cytoskeletal networkSoft Matter2020ISSN 1744-6848
Nakos, Konstantinos et al.Regulation of microtubule plus end dynamics by septin 9Cytoskeleton2019ISSN 1949-3592
Guedes-Dias, Pedro et al.Kinesin-3 Responds to Local Microtubule Dynamics to Target Synaptic Cargo Delivery to the PresynapseCurrent Biology2019ISSN 0960-9822
Nakos, Konstantinos et al.Septin 2/6/7 complexes tune microtubule plus-end growth and EB1 binding in a concentration- And filament-dependent mannerMolecular Biology of the Cell2019ISSN 1939-4586
Fan, Yuanwei et al.The Arabidopsis SPIRAL2 Protein Targets and Stabilizes Microtubule Minus EndsCurrent Biology2018ISSN 0960-9822
Colin, Alexandra et al.Actin-Network Architecture Regulates Microtubule DynamicsCurrent Biology2018ISSN 0960-9822
McClintock, Mark A. et al.RNA-directed activation of cytoplasmic dynein-1 in reconstituted transport RNPseLife2018ISSN 2050-084X
Aher, Amol et al.CLASP Suppresses Microtubule Catastrophes through a Single TOG DomainDevelopmental Cell2018ISSN 1878-1551
Melo, Esther et al.HtrA1 Mediated Intracellular Effects on Tubulin Using a Polarized RPE Disease ModelEBioMedicine2018ISSN 2352-3964
Zhu, Yili et al.An in vitro Microscopy-based Assay for Microtubule-binding and Microtubule-crosslinking by Budding Yeast Microtubule-associated ProteinBio-Protocol2018ISSN 2331--8325
Zhang, Rui et al.Interplay of structure, elasticity, and dynamics in actin-based nematic materialsProceedings of the National Academy of Sciences of the United States of America2017ISSN 1091-6490
Kandel, Mikhail E. et al.Label-Free Imaging of Single Microtubule Dynamics Using Spatial Light Interference MicroscopyACS Nano2017ISSN 1936-086X
Arellano-Santoyo, Hugo et al.A Tubulin Binding Switch Underlies Kip3/Kinesin-8 Depolymerase ActivityDevelopmental Cell2017ISSN 1878-1551
Britto, Mishan et al.Schizosaccharomyces pombe kinesin-5 switches direction using a steric blocking mechanismProceedings of the National Academy of Sciences of the United States of America2016ISSN 1091-6490
Taberner, Núria et al.Reconstituting Functional Microtubule-Barrier InteractionsMethods in Cell Biology2014ISSN 0091-679X
DeBerg, Hannah A. et al.Motor domain phosphorylation modulates kinesin-1 transportJournal of Biological Chemistry2013ISSN 0021-9258
Rostovtseva, Tatiana K. et al.Membrane Lipid Composition Regulates Tubulin Interaction with Mitochondrial Voltage-dependent Anion Channel *Journal of Biological Chemistry2012ISSN 0021--9258
Hara, Masatoshi et al.Greatwall kinase and cyclin B-Cdk1 are both critical constituents of M-phase-promoting factorNature Communications2012ISSN 2041-1723
  1. Tubulin protein (fluorescent AMCA): porcine brain TL440M
    Tubulin protein (fluorescent AMCA): porcine brain
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  2. Tubulin protein (fluorescent HiLyte 488): porcine brain TL488M
    Tubulin protein (fluorescent HiLyte 488): porcine brain
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  3. Tubulin protein (fluorescent HiLyte 647): porcine brain TL670M
    Tubulin protein (fluorescent HiLyte 647): porcine brain Learn More
  4. Tubulin protein (rhodamine): porcine brain TL590M
    Tubulin protein (rhodamine): porcine brain
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  5. Tubulin protein (X-rhodamine): porcine brain TL620M

    Tubulin protein (X-rhodamine): porcine brain

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