Protease inhibitor cocktail

Material
Protease Inhibitor Cocktail is supplied as a white lyophilized powder. The cocktail contains the following protease inhibitors, Pepstatin A, Leupeptin, Benzamidine, Na-p-tosyl-L-arginine methyl ester (TAME). At 1X final working concentration the inhibitors are at 1 µM, 1.5 µM, 1 mM and 0.4 mM respectively.

Mode of Action
pepstatin A Reversibly inhibits some aspartic proteases
Leupeptin Reversibly inhibits trypsin like serine and some cysteine proteases
Benzamidine Inhibits serine proteases
TAME Inhibits arginine proteases, a thrombin and trypsin inhibitor

Storage and Reconstitution
Lyophilized Protease Inhibitor Cocktail is stable at 4°C desiccated (<10% humidity) for 1 year.
Each tube of Protease Inhibitor Cocktail should be resuspended in 1ml of DMSO (dimethyl sulfoxide) to give a 100X strength stock. The reconstituted stock can be stored at 4°C for up to 6 months or at -70°C for up to 1 year.

Related Products
This protease inhibitor cocktail is used in the following Cytoskeleton Activation Assays:

• Ras activation assay Biochem kit (Cat. # BK008)
• Cdc42 activation assay Biochem kit (Cat. # BK034)
• Rac activation assay Biochem kit (Cat. # BK035)
• RhoA activation assay Biochem kit (Cat. # BK036)
• G-actin : F-actin in vivo assay Biochem kit (Cat. # BK037)
• Microtubule : tubulin in vivo assay Biochem kit (Cat. # BK038)
• RhoA G-LISA™ Activation Assay Kit (luminescence) (Cat. # BK121)
• RhoA G-LISA™ Activation Assay Kit (colorimetric) (Cat. # BK124)
• Rac1,2,3 G-LISA™ Activation Assay Kit (colorimetric) (Cat. # BK125)
• Rac1 G-LISA™ Activation Assay Kit (luminescence) (Cat. # BK126)
• Cdc42 G-LISA™ Activation Assay (Cat. # BK127)
• Rac1 G-LISA™ Activation Assay (Colorimetric Based) (Cat. # BK128)
• RalA G-LISA™ Activation Assay (Colorimetric Based) (Cat. # BK129)