Advanced Protein Assay: 5x Stock Detergent Compatability (Cat. # ADV01)
Chemical group /chemical name | Tested concentration that does not alter protein assay color(using 10 µl per ml ADV01) | Tested concentration that does not alter protein assay response to protein (using 10 µl per ml ADV01) |
Buffers / Tris pH 8.0 | >1.0 M | >1.0 M |
Buffers / HEPES pH 8.0 | >1.0 M | >1.0 M |
Buffers / PIPES pH 7.0 | >1.0 M | >1.0 M |
Buffers / Potassium phosphate pH7.0 | >1.0 M | >1.0 M |
Buffers / Sodium bicarbonate pH9.5 | >1.0 M | >1.0 M |
Reducing agents / BME | >1.0 M | >1.0 M |
Reducing agents / DTT | >1.0 M | >1.0 M |
Reducing agents / monothioglycerol | >1.0 M | >1.0 M |
Denaturants / 8 M urea | 100% | 100% |
Denaturants / 5 M guanidine-HCl | 100% | 100% |
Divalent cations / MgCl2 | >1.0 M | >1.0 M |
Divalent cations / CaCl2 | >1.0 M | >1.0 M |
Divalent cations / NiCl2 | >0.1 M | >0.1 M |
Chelating agents / EDTA | >1.0 M | >1.0 M |
Chelating agents / EGTA | >1.0 M | >1.0 M |
Detergents / SDS | 5.0% | 0.5% |
Detergents / NP40 | 0.5% | 0.2% |
Detergents / Triton X-100 | 0.2% | 0.2% |
Detergents / Tween 20 | 1.0% | 0.5% |
Solvents / DMSO | 100% | 100% |
Solvents / DMF | 100% | 100% |
Solvents / ethyl alcohol | 100% | 100% |
Solvents / methanol | 100% | 100% |
Antifoaming agent / Antifoam-C | 30% | 30% |
Acids / hydrochloric acid | >1.0 M | >1.0 M |
Acids / perchloric acid | >1.0 M | >1.0 M |
Acids / trichloric acid | >1.0 M | >1.0 M |
Acids / nitric acid | >1.0 M | >1.0 M |
Acids / sulfuric acid | >1.0 M | >1.0 M |