Precision Red Advanced Protein Assay: 1x Stock Detergent Compatability (Cat. # ADV02)

Chemical group /chemical name

Tested concentration that does not alter protein assay color(using 10µl per ml ADV02)

Tested concentration that does not alter protein assay response to protein (using 10µl per ml ADV02)

Buffers / Tris pH 8.0

>1.0 M

>1.0 M

Buffers / HEPES pH 8.0

>1.0 M

>1.0 M

Buffers / PIPES pH 7.0

>1.0 M

>1.0 M

Buffers / Potassium phosphate pH7.0

>1.0 M

>1.0 M

Buffers / Sodium bicarbonate pH9.5

>1.0 M

>1.0 M

Reducing agents / BME

>1.0 M

>1.0 M

Reducing agents / DTT

>1.0 M

>1.0 M

Reducing agents / monothioglycerol

>1.0 M

>1.0 M

Denaturants / 8 M urea

100%

100%

Denaturants / 5 M guanidine-HCl

100%

100%

Divalent cations / MgCl2

>1.0 M

>1.0 M

Divalent cations / CaCl2

>1.0 M

>1.0 M

Divalent cations / NiCl2

>0.1 M

>0.1 M

Chelating agents / EDTA

>1.0 M

>1.0 M

Chelating agents / EGTA

>1.0 M

>1.0 M

Detergents / SDS

0.1%

0.1%

Detergents / NP40

10%

10%

Detergents / Triton X-100

10%

0.5%

Detergents / Tween 20

10%

10%

Solvents / DMSO

100%

100%

Solvents / DMF

100%

100%

Solvents / ethyl alcohol

100%

100%

Solvents / methanol

100%

100%

Antifoaming agent / Antifoam-C

10%

10%

Acids / hydrochloric acid

>1.0 M

>0.75 M

Acids / perchloric acid

>1.0 M

>0.75 M

Acids / trichloric acid

>1.0 M

>0.50 M

Acids / nitric acid

>1.0 M

>0.75 M

Acids / sulfuric acid

>1.0 M

>0.25 M