Protease inhibitor cocktail

Protease inhibitor cocktail

Product Uses Include

  • Inhibition of a wide range of proteases.
  • Popular Activation Assay Component

Protease Inhibitor Cocktail is supplied as a white lyophilized powder. The cocktail contains the following protease inhibitors, Pepstatin A, Leupeptin, Benzamidine, Na-p-tosyl-L-arginine methyl ester (TAME). At 1X final working concentration the inhibitors are at 1 µM, 1.5 µM, 1 mM and 0.4 mM respectively.

Mode of Action
pepstatin A Reversibly inhibits some aspartic proteases
Leupeptin Reversibly inhibits trypsin like serine and some cysteine proteases
Benzamidine Inhibits serine proteases
TAME Inhibits arginine proteases, a thrombin and trypsin inhibitor

Storage and Reconstitution
Lyophilized Protease Inhibitor Cocktail is stable at 4°C desiccated (<10% humidity) for 1 year.
Each tube of Protease Inhibitor Cocktail should be resuspended in 1ml of DMSO (dimethyl sulfoxide) to give a 100X strength stock. The reconstituted stock can be stored at 4°C for up to 6 months or at -70°C for up to 1 year.

Related Products
This protease inhibitor cocktail is used in the following Cytoskeleton Activation Assays:

  • Ras activation assay Biochem kit (Cat. # BK008)
  • Cdc42 activation assay Biochem kit (Cat. # BK034)
  • Rac activation assay Biochem kit (Cat. # BK035)
  • RhoA activation assay Biochem kit (Cat. # BK036)
  • G-actin : F-actin in vivo assay Biochem kit (Cat. # BK037)
  • Microtubule : tubulin in vivo assay Biochem kit (Cat. # BK038)
  • RhoA G-LISA™ Activation Assay Kit (luminescence) (Cat. # BK121)
  • RhoA G-LISA™ Activation Assay Kit (colorimetric) (Cat. # BK124)
  • Rac1,2,3 G-LISA™ Activation Assay Kit (colorimetric) (Cat. # BK125)
  • Rac1 G-LISA™ Activation Assay Kit (luminescence) (Cat. # BK126)
  • Cdc42 G-LISA™ Activation Assay (Cat. # BK127)
  • Rac1 G-LISA™ Activation Assay (Colorimetric Based) (Cat. # BK128)
  • RalA G-LISA™ Activation Assay (Colorimetric Based) (Cat. # BK129)

For product Datasheets and MSDSs please click on the PDF links below.   For additional information, click on the FAQs tab above or contact our Technical Support department at

V. Munnamalai et al. 2014. Bidirectional interactions between NOX2-type NADPH oxidase and the F-actin cytoskeleton in neuronal growth cones. J. Neurochem. doi: 10.1111/jnc.12734.

H. Vindin et al. 2013. Validation of an algorithm to quantify changes in actin cytoskeletal organization. J. Biomol. Screen. 19, 354-68.

D. Chand et al. 2012. C-terminal region of teneurin-1 co-localizes with dystroglycan and modulates cytoskeletal organization through an extracellular signal-regulated kinase-dependent stathmin- and filamin A-mediated mechanism in hippocampal cells. Neurosci. 219, 255–270.

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