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Paclitaxel (Taxol)

Paclitaxel (Taxol)
$0.00
SKU
TXD01
Datasheet
MSDS
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Product Uses Include

  • Promote tubulin polymerization in vitro
  • Promote microtubule stability in vitro
  • Anti-mitotic drug in cell cultures
Click Here for Detailed Methods

Material
Paclitaxel's (Taxol) usefulness as a laboratory tool is well established and lies in its ability to inhibit microtubule depolymerization. Thus, taxol stabilized microtubules are used as substrates for the identification and characterization of the ever increasing number of microtubule associated proteins.

Paclitaxel is provided as a lyophilized powder. When resuspended in DMSO (not included) the paclitaxel is at 2 mM concentration.

Purity
In keeping with our policy of providing only the highest quality products, we are now pleased to inform you that Cytoskeleton is offering paclitaxel (taxol), from the Pacific yew tree, Taxus brevifolia, at a chromatographic purity of >99.5%. This is the purest taxol commercially available for research use.

Biological Activity
We have determined that the microtubule stabilizing property of our product is equal or superior to any other commercially available taxols. Microtubules in 10 µM paclitaxel are stable at room temperature for over one week.

For product Datasheets and MSDSs please click on the PDF links below.   For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com

AuthorTitleJournalYearArticle Link
Robinson, Benjamin P. et al.Septin-coated microtubules promote maturation of multivesicular bodies by inhibiting their motilityJournal of Cell Biology2024
Babcock, N. S. et al.Ultraviolet Superradiance from Mega-Networks of Tryptophan in Biological ArchitecturesJournal of Physical Chemistry B2024
Ceglowski, Julia et al.TTLL12 is required for primary ciliary axoneme formation in polarized epithelial cellsEMBO Reports2024
Fan, Yuanwei et al.A divergent tumor overexpressed gene domain and oligomerization contribute to SPIRAL2 function in stabilizing microtubule minus endsThe Plant Cell2024
Narwanti, Iin et al.6-Regioisomeric 5,8-quinolinediones as potent CDC25 inhibitors against colorectal cancersEuropean Journal of Medicinal Chemistry2023
Pyrpassopoulos, Serapion et al.KIF1A is kinetically tuned to be a superengaging motor under hindering loadsProceedings of the National Academy of Sciences2023
Bleiler, Marina et al.Incorporation of 53BP1 into phase-separated bodies in cancer cells during aberrant mitosisJournal of Cell Science2023
Ortmann, Simon et al.A conserved microtubule-binding region in Xanthomonas XopL is indispensable for induced plant cell death reactionsPLOS Pathogens2023
Hsieh, Yao Yu et al.Repositioning VU-0365114 as a novel microtubule-destabilizing agent for treating cancer and overcoming drug resistanceMolecular Oncology2023
Hoshino, Asumi et al.The microtubule-severing protein UNC-45A preferentially binds to curved microtubules and counteracts the microtubule-straightening effects of TaxolJournal of Biological Chemistry2023
Hoti, Qendresa et al.Avermectin B1a Shows Potential Anti-Proliferative and Anticancer Effects in HCT-116 Cells via Enhancing the Stability of MicrotubulesCurrent Issues in Molecular Biology 2023
Guerra San Juan, Irune et al.Loss of mouse Stmn2 function causes motor neuropathyNeuron2022
Feizabadi, Mitra Shojania et al.The Effect of Tau and Taxol on Polymerization of MCF7 Microtubules In VitroInternational Journal of Molecular Sciences2022
Gibson, James M et al.Coil-to-α-helix transition at the Nup358-BicD2 interface activates BicD2 for dynein recruitmenteLife2022
Pizzi, Rita et al.Evidences of new biophysical properties of microtubulesFocus on Artificial Neural Networks2021
Sinclair, Amy N. et al.The Trypanosoma brucei subpellicular microtubule array is organized into functionally discrete subdomains defined by microtubule associated proteinsPLOS Pathogens2021
Chang, Ting Yu et al.A novel histone deacetylase inhibitor MPT0L184 dysregulates cell-cycle checkpoints and initiates unscheduled mitotic signalingBiomedicine and Pharmacotherapy2021
Habicht, Juri et al.UNC-45A breaks the microtubule lattice independently of its effects on non-muscle myosin IIJournal of Cell Science2021
Qiu, Rongde et al.Dynein activation in vivo is regulated by the nucleotide states of its AAA3 domainCurrent Biology2021
Kaur, Simranpreet et al.Expansion of the phenotypic spectrum of de novo missense variants in kinesin family member 1A (KIF1A)Human Mutation2020
Li, Faxiang et al.Cryo-em structure of vash1-svbp bound to microtubuleseLife2020
Hao, Huiwen et al.Golgi‐associated microtubules are fast cargo tracks and required for persistent cell migrationEMBO reports2020
Ait-Bouziad, Nadine et al.Phosphorylation of the overlooked tyrosine 310 regulates the structure, aggregation, and microtubule- And lipid-binding properties of TauJournal of Biological Chemistry2020
Kalra, Aarat P. et al.Investigation of the electrical properties of microtubule ensembles under cell-like conditionsNanomaterials2020
Chiolerio, Alessandro et al.On resistance switching and oscillations in tubulin microtubule dropletsJournal of Colloid and Interface Science2020
Zhou, Chen et al.Structural basis of tubulin detyrosination by VASH2/SVBP heterodimerNature Communications2019
Schimert, Kristin I. et al.Intracellular cargo transport by single-headed kinesin motorsProceedings of the National Academy of Sciences of the United States of America2019
Huang, Chao et al.FoxM1 Induced Pac******* Resistance via Activation of the FoxM1/PHB1/RAF-MEK-ERK Pathway and Enhancement of the ABCA2 TransporterMolecular Therapy - Oncolytics2019
Guedes-Dias, Pedro et al.Kinesin-3 Responds to Local Microtubule Dynamics to Target Synaptic Cargo Delivery to the PresynapseCurrent Biology2019
Li, Feiran et al.Local direction change of surface gliding microtubulesBiotechnology and Bioengineering2019
Tjioe, Marco et al.Multiple kinesins induce tension for smooth cargo transporteLife2019
Budaitis, Breane G. et al.Neck linker docking is critical for kinesin-1 force generation in cells but at a cost to motor speed and processivityeLife2019
Cloer, E. W. et al.p62-Dependent Phase Separation of Patient-Derived KEAP1 Mutations and NRF2Molecular and Cellular Biology2018
Qiang, Liang et al.Tau Does Not Stabilize Axonal Microtubules but Rather Enables Them to Have Long Labile DomainsCurrent Biology2018
Ganguly, Anindya et al.Importin-β Directly Regulates the Motor Activity and Turnover of a Kinesin-4Developmental Cell2018
Sladewski, Thomas E. et al.Recruitment of two dyneins to an mRNA-dependent bicaudal D transport complexeLife2018
McIntosh, Betsy B. et al.Opposing Kinesin and Myosin-I Motors Drive Membrane Deformation and Tubulation along Engineered Cytoskeletal NetworksCurrent Biology2018
Reinemann, Dana N. et al.Processive Kinesin-14 HSET Exhibits Directional Flexibility Depending on Motor TrafficCurrent Biology2018
Melo, Esther et al.HtrA1 Mediated Intracellular Effects on Tubulin Using a Polarized RPE Disease ModelEBioMedicine2018
Chen, Yang et al.Visualizing Autophagic Lysosome Reformation in Cells Using In Vitro Reconstitution SystemsCurrent Protocols in Cell Biology2018
Bajaj, Rakhi et al.KNL1 Binding to PP1 and Microtubules Is Mutually ExclusiveStructure2018
Zhu, Yili et al.An in vitro Microscopy-based Assay for Microtubule-binding and Microtubule-crosslinking by Budding Yeast Microtubule-associated ProteinBio-Protocol2018
Su, Qian Peter et al.Corrigendum: Vesicle Size Regulates Nanotube Formation in the CellScientific reports2017
Kandel, Mikhail E. et al.Label-Free Imaging of Single Microtubule Dynamics Using Spatial Light Interference MicroscopyACS Nano2017
Howes, Stuart C. et al.Structural differences between yeast and mammalian microtubules revealed by cryo-EMJournal of Cell Biology2017
Reinemann, Dana N. et al.Collective Force Regulation in Anti-parallel Microtubule Gliding by Dimeric Kif15 Kinesin MotorsCurrent Biology2017
Zollo, Massimo et al.PRUNE is crucial for normal brain development and mutated in microcephaly with neurodevelopmental impairmentBrain2017
Kellogg, Elizabeth H. et al.Near-atomic cryo-EM structure of PRC1 bound to the microtubuleProceedings of the National Academy of Sciences of the United States of America2016
Du, Wanqing et al.Kinesin 1 Drives Autolysosome TubulationDevelopmental Cell2016
Matsuyama, Tomonori et al.Midaz(olam) inhibits the hypoxia-induced up-regulation of erythropoietin in the central nervous systemEuropean Journal of Pharmacology2015
Wang, Chong et al.Dynamic tubulation of mitochondria drives mitochondrial network formationCell Research2015
Batalla Mayoral, J. et al.Potencial zeta en la determinación de carga superficial de liposomas.Latin-American Journal of Physics Education2014
Howes, Stuart C. et al.Effects of tubulin acetylation and tubulin acetyltransferase binding on microtubule structureMolecular Biology of the Cell2014
Szyk, Agnieszka et al.Molecular basis for age-dependent microtubule acetylation by tubulin acetyltransferaseCell2014
Makrantoni, Vasso et al.Phosphorylation of Sli15 by Ipl1 is important for proper CPC localization and chromosome stability in Saccharomyces cerevisiaePLoS ONE2014
Kadakkuzha, Beena M. et al.High-throughput screening for small molecule modulators of motor protein kinesinAssay and Drug Development Technologies2014
Lee, Dongmin et al.Enhanced expression and purification of inositol 1,4,5-trisphosphate 3-kinase A through use of the pCold1-GST vector and a C-terminal hexahistidine tag in Escherichia coliProtein Expression and Purification2014
Hernández Candia, Carmen Noemí et al.A Minimal Optical Trapping and Imaging Microscopy SystemPLoS ONE2013
Lee, D et al.Inositol 1, 4, 5-Trisphosphate 3-Kinase A Is a Novel Microtubule-associated ProteinJournal of Biological …2012
Dempsey, Graham T. et al.Evaluation of fluorophores for optimal performance in localization-based super-resolution imagingNature Methods2011
Gutiérrez-Medina, Braulio et al.Visualizing individual microtubules by bright field microscopyAmerican Journal of Physics2010
Teckchandani, Anjali M. et al.The multidomain protooncogenic protein c-Cbl binds to tubulin and stabilizes microtubulesExperimental cell research2005
Kosturko, Linda D. et al.The Microtubule-associated Protein Tumor Overexpressed Gene Binds to the RNA Trafficking Protein Heterogeneous Nuclear Ribonucleoprotein A2Molecular Biology of the Cell2005
Nair, K. Saidas et al.Direct Binding of Visual Arrestin to Microtubules Determines the Differential Subcellular Localization of Its Splice Variants in Rod Photoreceptors *Journal of Biological Chemistry2004
Ligon, Lee A. et al.The Microtubule Plus-End Proteins EB1 and Dynactin Have Differential Effects on Microtubule PolymerizationMolecular Biology of the Cell2003
Benink, H. A. et al.Analysis of Cortical Flow Models In VivoMolecular Biology of the Cell2000
Korinek, William S. et al.Molecular linkage underlying microtubule orientation toward cortical sites in yeastScience (New York, N.Y.)2000

 

Question 1: Can I mix taxol with my cell culture media and store at 4°C?

Answer 1: No, we do not recommend storing cell culture media with taxol (Cat. # TXD01).  The taxol could precipitate out of solution at 4°C since the taxol is prepared in a 100% DMSO solution.  Instead, add taxol to warm cell culture media to be used on the day of the experiment.

 

Question 2: Can I incubate my cells with taxol to affect in vivo tubulin polymerization?

Answer 2: In-house testing demonstrates that 1 hour with 0.5 or 1 mM taxol (Cat. # TXD01) produces a robust increase in microtubules in Swiss 3T3 fibroblast cells.

 

 

If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com

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