Acetyl-Lysine Affinity Beads - AAC02

Application 1: Detection of Acetylation Changes in a Target POI

AAC02-Beads have been shown to be a superior reagent for the immunoprecipitation of acetylated PDHE1 (Fig. 1). In the presence of hydrogen peroxide AAC02 Bead IPs show a 2.6 fold de-acetylation of PDHE1 while AAC03 Bead shows a 1.5 fold de-acetylation and 8-9 fold weaker signal. The specificity of the beads for acetylated PDHE1 is shown by the lack of signal from mouse IgG control beads (Fig. 1). A potential mechanism for deacetylation in response to hydrogen peroxide treatment is the upregulation of the deacetylase SIRT3.

Figure 1 Legend: AAC02-Beads & AAC03-Beads (50μl bead slurry) were used to IP acetylated proteins from A431 cell lysates either treated (+) or untreated (-) with hy-drogen peroxide (100 μM) for 2 hours. Each IP used 1 mg of lysate. Western blot analysis using anti PDHE1 antibody was performed and signals were quantitated using LiCor Empiria software (Table). Mouse IgG control beads (Cat #CIG02) and AAC04-Beads (25 μl AAC02-Beads & 25 μl AAC03-Beads) were also used in IP assays (1 mg lysate per IP). Input lanes repre-sent 2% of IP input lysate (20 μg) from treated (+) or untreated (-) lysate. Input signal represents total PDHE1.