GTP: 100mM stock

GTP: 100mM stock
$0.00

Product Uses Include 

  • Stabilise and promote polymerization of tubulin Substrate for GTPases

 

Material
Guanosine triphosphate (GTP). 100 mM solution of GTP. Provided as a lyophilized powder. Required for tubulin stability and microtubule assembly and dynamics.

For product Datasheets and MSDSs please click on the PDF links below.   For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com

AuthorTitleJournalYearArticle Link
Piroli, Gerardo G. et al.Defective function of α-ketoglutarate dehydrogenase exacerbates mitochondrial ATP deficits during complex I deficiencyRedox Biology2023ISSN 2213--2317
Singh, Rajendra K. et al.Coordinated Biophysical Stimulation of MSCs via Electromagnetized Au-Nanofiber Matrix Regulates Cytoskeletal-to-Nuclear Mechanoresponses and Lineage SpecificationAdvanced Functional Materials2023ISSN 1616--3028
Feizabadi, Mitra Shojania et al.The Effect of Tau and Taxol on Polymerization of MCF7 Microtubules In VitroInternational Journal of Molecular Sciences2022ISSN 1422-0067
Wu, R. et al.Thermal hysteresis in microtubule assembly/disassembly dynamics: The aging-induced degradation of tubulin dimersBiochemistry and Biophysics Reports2022ISSN 2405-5808
Pizzi, Rita et al.Evidences of new biophysical properties of microtubulesFocus on Artificial Neural Networks2021Article Link
Hough, Cameron M. et al.Disassembly of microtubules by intense terahertz pulsesBiomedical Optics Express2021ISSN 2156--7085
Qiu, Rongde et al.Dynein activation in vivo is regulated by the nucleotide states of its AAA3 domainCurrent Biology2021ISSN 1879-0445
Li, Yaxin et al.Small-Molecule HSP27 Inhibitor Abolishes Androgen Receptors in GlioblastomaJournal of Medicinal Chemistry2021ISSN 1520-4804
Kaur, Simranpreet et al.Expansion of the phenotypic spectrum of de novo missense variants in kinesin family member 1A (KIF1A)Human Mutation2020ISSN 1098-1004
Baker, Stacey J. et al.A Contaminant Impurity, Not Rigosertib, Is a Tubulin Binding AgentMolecular Cell2020ISSN 1097-4164
Kalra, Aarat P. et al.Investigation of the electrical properties of microtubule ensembles under cell-like conditionsNanomaterials2020ISSN 2079-4991
Budaitis, Breane G. et al.Neck linker docking is critical for kinesin-1 force generation in cells but at a cost to motor speed and processivityeLife2019ISSN 2050-084X
Reinemann, Dana N. et al.Processive Kinesin-14 HSET Exhibits Directional Flexibility Depending on Motor TrafficCurrent Biology2018ISSN 0960-9822
Melo, Esther et al.HtrA1 Mediated Intracellular Effects on Tubulin Using a Polarized RPE Disease ModelEBioMedicine2018ISSN 2352-3964
Chen, Yang et al.Visualizing Autophagic Lysosome Reformation in Cells Using In Vitro Reconstitution SystemsCurrent Protocols in Cell Biology2018ISSN 1934-2616
Bajaj, Rakhi et al.KNL1 Binding to PP1 and Microtubules Is Mutually ExclusiveStructure2018ISSN 1878-4186
Zhu, Yili et al.An in vitro Microscopy-based Assay for Microtubule-binding and Microtubule-crosslinking by Budding Yeast Microtubule-associated ProteinBio-Protocol2018ISSN 2331--8325
Su, Qian Peter et al.Corrigendum: Vesicle Size Regulates Nanotube Formation in the CellScientific reports2017ISSN 2045-2322
Reinemann, Dana N. et al.Collective Force Regulation in Anti-parallel Microtubule Gliding by Dimeric Kif15 Kinesin MotorsCurrent Biology2017ISSN 0960-9822
Du, Wanqing et al.Kinesin 1 Drives Autolysosome TubulationDevelopmental Cell2016ISSN 1878-1551
Wang, Chong et al.Dynamic tubulation of mitochondria drives mitochondrial network formationCell Research2015ISSN 1748-7838
Makrantoni, Vasso et al.Phosphorylation of Sli15 by Ipl1 is important for proper CPC localization and chromosome stability in Saccharomyces cerevisiaePLoS ONE2014ISSN 1932-6203
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Xiao, Xiang et al.c-Yes regulates cell adhesion at the apical ectoplasmic specialization-blood-testis barrier axis via its effects on protein recruitment and distribution2013PMID 23169788
Hartley, R. M. et al.Polygamain, a New Microtubule Depolymerizing Agent That Occupies a Unique Pharmacophore in the Colch***** SiteMolecular Pharmacology2012ISSN 0026-895X
Chen, Chun Han et al.Protopine, a novel microtubule-stabilizing agent, causes mitotic arrest and apoptotic cell death in human hormone-refractory prostate cancer cell lines2012PMID 22033245
Butler, Suzanne C. et al.Inhibitory effects of pectenotoxins from marine algae on the polymerization of various actin isoformsToxicology in Vitro2012ISSN 0887-2333
Lee, D et al.Inositol 1, 4, 5-Trisphosphate 3-Kinase A Is a Novel Microtubule-associated ProteinJournal of Biological …2012Article Link
Carletti, B. et al.Effect of protein glutathionylation on neuronal cytoskeleton: a potential link to neurodegenerationNeuroscience2011ISSN 1873--7544
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Chang, Chia Hao et al.Mycotoxin citrinin induced cell cycle G2/M arrest and numerical chromosomal aberration associated with disruption of microtubule formation in human cellsToxicological sciences : an official journal of the Society of Toxicology2011ISSN 1096--0929
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Question 1: Does GTP need to be included in tubulin buffers prepared for reconstituting and storing tubulin if the tubulin will not be used for polymerization?

Answer 1:  Cytoskeleton’s tubulin proteins are lyophilized in a buffer that contains 1 mM GTP, so omitting additional GTP (Cat. # BST06) in the reconstitution buffer can be done if the tubulin will not be used for polymerization.  However, this is not recommended.  Under most preparation conditions, GTP is required for tubulin stability and for the formation of microtubules in vivo and in vitro and we recommend adding fresh GTP (1 mM) to the PEM buffer used for reconstitution and polymerization.

 

Question 2: How long is GTP-containing tubulin buffer stable for when stored on ice the day of the experiment?

Answer 2: Buffers that contain GTP (Cat. # BST06) should not be kept longer than 2 hours on ice.  After this time, GTP-supplemented buffers should be discarded. Do not attempt to freeze and re-use these buffers. 

 

If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com