Cytoskeleton now offers the first and only commercially available RhoA ELISA.
It is recommended to use the BK150 kit in conjunction with the RhoA G-LISA™ kit (BK124), allowing quantitation of Total RhoA and Active RhoA in the same lysates.
Providing precise solutions to RhoA quantification, enabling researchers to...
Kit Contents - Enough reagents for 96 assays.
|Lee, Sang Joon et al.||AIM2 forms a complex with pyrin and ZBP1 to drive PANoptosis and host defence||Nature||2021||ISSN 1476-4687|
|Porter, Lauren et al.||SUN1/2 Are Essential for RhoA/ROCK-Regulated Actomyosin Activity in Isolated Vascular Smooth Muscle Cells||Cells||2020||ISSN 2073-4409|
|Ngai, David et al.||DDR1 (Discoidin Domain Receptor-1)-RhoA (Ras Homolog Family Member A) Axis Senses Matrix Stiffness to Promote Vascular Calcification||Arteriosclerosis, Thrombosis, and Vascular Biology||2020||ISSN 1524-4636|
|Lachowski, Dariusz et al.||G Protein-Coupled Estrogen Receptor Regulates Actin Cytoskeleton Dynamics to Impair Cell Polarization||Frontiers in Cell and Developmental Biology||2020||ISSN 2296-634X|
|Chronopoulos, Antonios et al.||Syndecan-4 tunes cell mechanics by activating the kindlin-integrin-RhoA pathway||Nature Materials||2020||ISSN 1476-4660|
|Haq, Naila et al.||Loss of Bardet-Biedl syndrome proteins causes synaptic aberrations in principal neurons||PLoS Biology||2019||ISSN 1545-7885|
|Dér, Bálint et al.||NK2 receptor-mediated detrusor muscle contraction involves Gq/11-dependent activation of voltage-dependent Ca2+ channels and the RhoA-Rho kinase pathway||American Journal of Physiology - Renal Physiology||2019||ISSN 1522-1466|
|Zhang, Xiaoqing et al.||Alterations of MEK1/2-ERK1/2, IFNγ and Smad2/3 associated Signalling pathways during cryopreservation of ASCs affect their differentiation towards VSMC-like cells||Stem Cell Research||2018||ISSN 1876-7753|
|Giannini, Marianna et al.||Nano-topography: Quicksand for cell cycle progression?||Nanomedicine: Nanotechnology, Biology, and Medicine||2018||ISSN 1549-9642|
|Peng, Jing Hua et al.||Geniposide and Chlorogenic Acid Combination Ameliorates Non-alcoholic Steatohepatitis Involving the Protection on the Gut Barrier Function in Mouse Induced by High-Fat Diet||Frontiers in Pharmacology||2018||ISSN 1663-9812|
|Tod, Jo et al.||Pro-migratory and TGF-β-activating functions of αvβ6 integrin in pancreatic cancer are differentially regulated via an Eps8-dependent GTPase switch||Journal of Pathology||2017||ISSN 1096-9896|
|Schillaci, Odessa et al.||Exosomes from metastatic cancer cells transfer amoeboid phenotype to non-metastatic cells and increase endothelial permeability: Their emerging role in tumor heterogeneity||Scientific Reports||2017||ISSN 2045-2322|
|Kempf, Anissa et al.||Control of Cell Shape, Neurite Outgrowth, and Migration by a Nogo-A/HSPG Interaction||Developmental Cell||2017||ISSN 1878-1551|
|Dyberg, Cecilia et al.||Rho-associated kinase is a therapeutic target in neuroblastoma||Proceedings of the National Academy of Sciences of the United States of America||2017||ISSN 1091-6490|
|Park, Yong Hwan et al.||Pyrin inflammasome activation and RhoA signaling in the autoinflammatory diseases FMF and HIDS||Nature Immunology||2016||ISSN 1529-2916|
|Al-Shboul, Othman||The role of the RhoA/ROCK pathway in gender-dependent differences in gastric smooth muscle contraction||Journal of Physiological Sciences||2016||ISSN 1880-6546|
|Jiang, Lisheng et al.||Selective activation of CB2 receptor improves efferocytosis in cultured macrophages||Life Sciences||2016||ISSN 1879-0631|
|Yuan, Xue et al.||Ciliary IFT80 balances canonical versus non-canonical hedgehog signalling for osteoblast differentiation||Nature Communications||2016||ISSN 2041-1723|
|Escuin, Sarah et al.||Rho-kinase-dependent actin turnover and actomyosin disassembly are necessary for mouse spinal neural tube closure||Journal of Cell Science||2015||ISSN 1477-9137|
|Skrbic, Biljana et al.||Lack of collagen VIII reduces fibrosis and promotes early mortality and cardiac dilatation in pressure overload in mice||Cardiovascular Research||2015||ISSN 1755-3245|
|Suen, J. Y. et al.||Pathway-selective antagonism of proteinase activated receptor 2||British Journal of Pharmacology||2014||ISSN 1476-5381|
|Herr, Michael J. et al.||Tetraspanin CD9 regulates cell contraction and actin arrangement via RhoA in human vascular smooth muscle cells||PLoS ONE||2014||ISSN 1932-6203|
|Biechler V., Stefanie V. et al.||The impact of flow-induced forces on the morphogenesis of the outflow tract||Frontiers in Physiology||2014||ISSN 1664-042X|
|Tan, Hong et al.||Fluid flow forces and rhoA regulate fibrous development of the atrioventricular valves||Developmental Biology||2013||ISSN 1095-564X|
|Valtcheva, Nadejda et al.||The orphan adhesion G protein-coupled receptor GPR97 regulates migration of lymphatic endothelial cells via the small GTPases RhoA and Cdc42||Journal of Biological Chemistry||2013||ISSN 0021-9258|
Question 1: Can I use the same lysate samples to measure total RhoA with this kit (Cat. # BK150) and activated RhoA samples in the G-LISA activation assay kit (Cat. # BK124)?
Answer 1: Yes, the same lysate samples can be used in both the total RhoA ELISA (Cat. # BK150) and RhoA G-LISA activation assay kit (Cat. # BK124). When preparing samples to be used with both kits, please use the lysis buffer (Part # GL36) that comes with the RhoA G-LISA assay kit. Generally G‐LISA samples have low protein concentrations, e.g., 0.3 to 0.6 mg/ml. At this concentration the samples are at the lower range of detection for the ELISA. Therefore we recommend using more lysate and less Sample Dilution Buffer when preparing G-LISA samples for the ELISA. To keep the final concentration higher, we recommend using 40 μl of G‐LISA extract plus 80 μl of Sample Dilution Buffer, which is sufficient for duplicate ELISA wells. In this case, also use the same Lysis:SDB ratio for the positive control protein solutions.
Question 2: I see that this ELISA uses a 96 well plate format. Do I have to use all 96 wells at one time or can I save the unused wells for use at a later date?
Answer 2: The 96 well plates are packaged as 12 x 8 well strips. Thus, each strip is removable and can be further broken down to a 1 well format for an assay. In this way, there is tremendous format flexibility since 1-96 wells can be used in an assay. It is imperative to keep the plate in the sealed desiccant bag with desiccant at all times. Move to room temperature 30 min prior to starting the assay. Open the bag and remove the number of strips or wells needed immediately prior to the start of the experiment, place the remaining strips/wells in the desiccated bag, reseal and return to storage at 4°C. Prepare and use the strips/wells as directed.
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