Total RhoA ELISA

Total RhoA ELISA
$0.00

Cytoskeleton now offers the first and only commercially available RhoA ELISA.

It is recommended to use the BK150 kit in conjunction with the RhoA G-LISA™ kit (BK124), allowing quantitation of Total RhoA and Active RhoA in the same lysates.

    • Companion to RhoA G-LISA™
    • Extremely sensitive
    • Multi-species detection
    • Pre-coated plate for high reproducibility
    Know Your Rho Package

    Kit Contents - Enough reagents for 96 assays.

    • 96 well Total Rho binding plate
      (12 strips of 8 wells each)
    • Anti-RhoA antibody
    • Secondary Antibody - HRP
    • Rho control protein
    • Dilution Buffer
    • Wash Buffer (PBST)
    • Antigen Presenting Buffer
    • HRP Detection Reagents

    For product Datasheets and MSDSs please click on the PDF links below.   For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com

    AuthorTitleJournalYearArticle Link
    Lee, Sang Joon et al.AIM2 forms a complex with pyrin and ZBP1 to drive PANoptosis and host defenceNature2021ISSN 1476-4687
    Porter, Lauren et al.SUN1/2 Are Essential for RhoA/ROCK-Regulated Actomyosin Activity in Isolated Vascular Smooth Muscle CellsCells2020ISSN 2073-4409
    Lachowski, Dariusz et al.G Protein-Coupled Estrogen Receptor Regulates Actin Cytoskeleton Dynamics to Impair Cell PolarizationFrontiers in Cell and Developmental Biology2020ISSN 2296-634X
    Chronopoulos, Antonios et al.Syndecan-4 tunes cell mechanics by activating the kindlin-integrin-RhoA pathwayNature Materials2020ISSN 1476-4660
    Ngai, David et al.DDR1 (Discoidin Domain Receptor-1)-RhoA (Ras Homolog Family Member A) Axis Senses Matrix Stiffness to Promote Vascular CalcificationArteriosclerosis, Thrombosis, and Vascular Biology2020ISSN 1524-4636
    Haq, Naila et al.Loss of Bardet-Biedl syndrome proteins causes synaptic aberrations in principal neuronsPLoS Biology2019ISSN 1545-7885
    Dér, Bálint et al.NK2 receptor-mediated detrusor muscle contraction involves Gq/11-dependent activation of voltage-dependent Ca2+ channels and the RhoA-Rho kinase pathwayAmerican Journal of Physiology - Renal Physiology2019ISSN 1522-1466
    Peng, Jing Hua et al.Geniposide and Chlorogenic Acid Combination Ameliorates Non-alcoholic Steatohepatitis Involving the Protection on the Gut Barrier Function in Mouse Induced by High-Fat DietFrontiers in Pharmacology2018ISSN 1663-9812
    Giannini, Marianna et al.Nano-topography: Quicksand for cell cycle progression?Nanomedicine: Nanotechnology, Biology, and Medicine2018ISSN 1549-9642
    Zhang, Xiaoqing et al.Alterations of MEK1/2-ERK1/2, IFNγ and Smad2/3 associated Signalling pathways during cryopreservation of ASCs affect their differentiation towards VSMC-like cellsStem Cell Research2018ISSN 1876-7753
    Tod, Jo et al.Pro-migratory and TGF-β-activating functions of αvβ6 integrin in pancreatic cancer are differentially regulated via an Eps8-dependent GTPase switchJournal of Pathology2017ISSN 1096-9896
    Schillaci, Odessa et al.Exosomes from metastatic cancer cells transfer amoeboid phenotype to non-metastatic cells and increase endothelial permeability: Their emerging role in tumor heterogeneityScientific Reports2017ISSN 2045-2322
    Kempf, Anissa et al.Control of Cell Shape, Neurite Outgrowth, and Migration by a Nogo-A/HSPG InteractionDevelopmental Cell2017ISSN 1878-1551
    Dyberg, Cecilia et al.Rho-associated kinase is a therapeutic target in neuroblastomaProceedings of the National Academy of Sciences of the United States of America2017ISSN 1091-6490
    Yuan, Xue et al.Ciliary IFT80 balances canonical versus non-canonical hedgehog signalling for osteoblast differentiationNature Communications2016ISSN 2041-1723
    Jiang, Lisheng et al.Selective activation of CB2 receptor improves efferocytosis in cultured macrophagesLife Sciences2016ISSN 1879-0631
    Al-Shboul, OthmanThe role of the RhoA/ROCK pathway in gender-dependent differences in gastric smooth muscle contractionJournal of Physiological Sciences2016ISSN 1880-6546
    Park, Yong Hwan et al.Pyrin inflammasome activation and RhoA signaling in the autoinflammatory diseases FMF and HIDSNature Immunology2016ISSN 1529-2916
    Skrbic, Biljana et al.Lack of collagen VIII reduces fibrosis and promotes early mortality and cardiac dilatation in pressure overload in miceCardiovascular Research2015ISSN 1755-3245
    Escuin, Sarah et al.Rho-kinase-dependent actin turnover and actomyosin disassembly are necessary for mouse spinal neural tube closureJournal of Cell Science2015ISSN 1477-9137
    Herr, Michael J. et al.Tetraspanin CD9 regulates cell contraction and actin arrangement via RhoA in human vascular smooth muscle cellsPLoS ONE2014ISSN 1932-6203
    Biechler V., Stefanie V. et al.The impact of flow-induced forces on the morphogenesis of the outflow tractFrontiers in Physiology2014ISSN 1664-042X
    Suen, J. Y. et al.Pathway-selective antagonism of proteinase activated receptor 2British Journal of Pharmacology2014ISSN 1476-5381
    Tan, Hong et al.Fluid flow forces and rhoA regulate fibrous development of the atrioventricular valvesDevelopmental Biology2013ISSN 1095-564X
    Valtcheva, Nadejda et al.The orphan adhesion G protein-coupled receptor GPR97 regulates migration of lymphatic endothelial cells via the small GTPases RhoA and Cdc42Journal of Biological Chemistry2013ISSN 0021-9258

    Question 1:  Can I use the same lysate samples to measure total RhoA with this kit (Cat. # BK150) and activated RhoA samples in the G-LISA activation assay kit (Cat. # BK124)?

    Answer 1:  Yes, the same lysate samples can be used in both the total RhoA ELISA (Cat. # BK150) and RhoA G-LISA activation assay kit (Cat. # BK124).  When preparing samples to be used with both kits, please use the lysis buffer (Part # GL36) that comes with the RhoA G-LISA assay kit.  Generally G‐LISA samples have low protein concentrations, e.g., 0.3 to 0.6 mg/ml.  At this concentration the samples are at the lower range of detection for the ELISA.  Therefore we recommend using more lysate and less Sample Dilution Buffer when preparing G-LISA samples for the ELISA.  To keep the final concentration higher, we recommend using 40 μl of G‐LISA extract plus 80 μl of Sample Dilution Buffer, which is sufficient for duplicate ELISA wells.  In this case, also use the same Lysis:SDB ratio for the positive control protein solutions.

     

    Question 2:  I see that this ELISA uses a 96 well plate format.  Do I have to use all 96 wells at one time or can I save the unused wells for use at a later date?

    Answer 2:  The 96 well plates are packaged as 12 x 8 well strips.  Thus, each strip is removable and can be further broken down to a 1 well format for an assay.  In this way, there is tremendous format flexibility since 1-96 wells can be used in an assay.  It is imperative to keep the plate in the sealed desiccant bag with desiccant at all times.  Move to room temperature 30 min prior to starting the assay.  Open the bag and remove the number of strips or wells needed immediately prior to the start of the experiment, place the remaining strips/wells in the desiccated bag, reseal and return to storage at 4°C.  Prepare and use the strips/wells as directed.

     

    If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com.