Anti-alpha/beta tubulin: sheep polyclonal

Anti-alpha/beta tubulin: sheep polyclonal
$0.00

Product Uses
This antibody is recommended for detection of tubulin in all yeast, fungi, and animal species (Fig. 2).
The following protocols have been tested with this antibody:

Western BlotImmunofluorescence
YesYes

RRID

AB_10708807

Material
The anti-tubulin antibody (Cat. # ATN02) is an affinity purified sheep polyclonal antibody that reacts to alpha and beta tubulin. The immunogen used for antibody production was a mixture of purified tubulins and therefore the antibody has broad species cross reactivity from yeast to humans. Porcine extract (Cat. # EXT03) is included as a positive control and ATN02 identifies a characteristic tubulin band at 55 kD on Western blots (see Fig. 1).  ATN02 is supplied as a lyophilized white powder.

 

Example Results

Western Blot of AAN01

Figure 1. Western blot analysis of anti-tubulin antibody.  Protein samples were separated by electrophoresis and transferred to PVDF membrane as described in the methods.  ATN02 antibody was diluted to 50 0 ng/ml (1:1000) for Western blot analysis.  Tubulin was detected in 10 µg of bovine brain extract (see arrow at 55 kD).  Molecular weight markers are from Invitrogen. 

Western Blot of ATN02

Figure 2. Western blot of cell extracts probed with anti-tubulin polyclonal antibody (Cat. # ATN02). Chemiluminescence detection of tubulin in extracts of Drosophila S2 cells (50 µg, lane 1) Xenopus A6 cells (50 µg, lane 2), mouse Swiss 3T3 cells (50 µg, lane 3), rat NRK cells (50 µg, lane 4), human HeLa cells (50 µg, lane 5), and bovine brain (50 µg, lane 6) at 55 kD (see arrow).  The blot was probed with a 500 ng/ml (1:1000) dilution of ATN02.

IF of AAN01

Figure 3. Immunofluorescence image of mouse Swiss 3T3 cells stained with Anti-Tubulin  polyclonal antibody (Cat. # ATN02). Swiss 3T3 cells were grown to semi-confluency and fixed with methanol. Immunofluorescence staining using 2.5 µg/ml (1:200 dilution) ATN02 antibody is shown (red). The primary antibody was detected with a 1:500 dilution of anti-sheep rhodamine conjugated antibody. DNA (blue) was stained with 100 nM DAPI in PBS. Photograph was taken with a 100X objective lens.

Immunofluorescence image using ATN02

Figure 4. Immunofluorescence image of Arabidopsis thaliana metaphase cell stained with Anti-Tubulin polyclonal antibody (Cat. # ATN02).
Microtubules are pseudocolored in green and chrmosomes in red.  

Image courtesy of Dr. Bo Liu, Department of Plant Biology, University of California, Davis.

For product Datasheets and MSDSs please click on the PDF links below.   For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com

Ibarrola-Vannucci, AK. Et al. Characterization and functional analysis of the proteins Prohibitin 1 and 2 in Trypanosoma cruzi  https://doi.org/10.1371/journal.pntd.0009322 (2021)

Dixon, C. et al. STING nuclear partners contribute to innate immune signaling responses https://doi.org/10.1016/j.isci.2021.103055 (2021)

Yang, Y., Li, N., Qiu, J., Ge, H. & Qin, X. Identification of the Repressive Domain of the Negative Circadian Clock Component CHRONO. Int. J. Mol. Sci. 21, 2469 (2020).

Samson, A. L. et al. MLKL trafficking and accumulation at the plasma membrane control the kinetics and threshold for necroptosis. Nat. Commun. 11, 1–17 (2020).

Gaire, S. K. et al. Accelerating multicolor spectroscopic single-molecule localization microscopy using deep learning. Biomed. Opt. Express 11, 2705–2721 (2020).

Batlle, Cristina et al. “hnRNPDL Phase Separation Is Regulated by Alternative Splicing and Disease-Causing Mutations Accelerate Its Aggregation.” Cell reports vol. 30,4 (2020): 1117-1128.e5. doi:10.1016/j.celrep.2019.12.080

Lemjabbar-Alaoui, H., Peto, C. J., Yang, Y.-W. & Jablons, D. M. AMXI-5001, a novel dual parp1/2 and microtubule polymerization inhibitor for the treatment of human cancers. Am. J. Cancer Res. 10, 2649–2676 (2020).

Ki-Hee Song et al. "Three-dimensional biplane spectroscopic single-molecule localization microscopy," Optica 6, 709-715 (2019)

Tejedo, Milvia Iris Alata et al. “3,3'-thiodipropanol as a versatile refractive index-matching mounting medium for fluorescence microscopy.” Biomedical optics express vol. 10,3 1136-1150. 11 Feb. 2019, doi:10.1364/BOE.10.001136

Xu, Xiaoxue et al. “Probenecid Disrupts a Novel Pannexin 1-Collapsin Response Mediator Protein 2 Interaction and Increases Microtubule Stability.” Frontiers in cellular neuroscience vol. 12 124. 11 May. 2018, doi:10.3389/fncel.2018.00124

Methods Mol. Biol. 2015; 1208, 395-408.
"Intracellular distribution of glutathionylated proteins in cultured dermal fibroblasts by immunofluorescence."
Author(s): Petrini S. et al. 
(See PubMed article)

Reproduction. 2015; 149, 633-44.
"USP8/UBPy-regulated sorting and the development of sperm acrosome: the recruitment of MET."
Author(s): Berruti G. and Paiardi C.
(See PubMed article)

Anti-a/b tubulin antibody (Cat. # ATN02) was used in Western blotting to examine if succination altered the immunodetectability of tubulin modified by this PTM.
Biochem. J. 462, 231-45.
"Identification of protein succination as a novel modification of tubulin"
Piroli G.G. et al. 
(See PubMed article)

Anti-a/b tubulin antibody (Cat. # ATN02) was used to immunocytochemically label tubulin in mammary epithelial cells in the study of the cellular response to DNA damage.
J. Biol. Chem. 2012; 287, 22838–22853.
"Elevated cyclin G2 expression intersects with DNA damage checkpoint signaling and is required for a potent G2/M checkpoint arrest response to doxorubicin."
Author(s): M. Zimmermann et al.
(See PubMed article)

Plant Cell. 2011; 23, 2606–2618
"Augmin plays a critical role in organizing the spindle and phragmoplast microtubule arrays in Arabidopsis."
Author(s): C.-M.K. Ho et al.
(See PubMed article)

Plant Cell. 2011; 23, 2909-2923.
"Interaction of antiparallel microtubules in the phragmoplast is mediated by the microtubule-associated protein MAP65-3 in Arabidopsis."
Author(s): C.-M.K. Ho et al.
(See PubMed article)

Planta. 2010; 231, 277-291.
"Changes in the accumulation of a- and b-tubulin during bud development in Vitis Vinifera L."
Author(s): L. Parrotta et al.
(See PubMed article)

J. Comp. Neurol. 2009; 515, 295-312.
"Axon regeneration in the absence of growth cones: Acceleration by cyclic AMP."
Author(s): Jin L.Q. et al. 
(See PubMed article)

J. Biol. Chem. 2008; 283, 26188-26197.
"Heat shock transcription factor 1-activating compounds suppress polyglutamine-induced neurodegeneration through induction of multiple molecular chaperones."
Author(s): Fujikake N. et al.
(See PubMed article)

 

 

AuthorTitleJournalYearArticle Link
Kriek, Neline et al.Cucurbitacins elicit anti-platelet activity via perturbation of the actin cytoskeleton and integrin function.Thrombosis and Haemostasis2022ISSN 0340--6245
Guerra San Juan, Irune et al.Loss of mouse Stmn2 function causes motor neuropathyNeuron2022ISSN 0896-6273
Xie, Xuan et al.α-TubK40me3 is required for neuronal polarization and migration by promoting microtubule formationNature Communications2021ISSN 2041-1723
Ibarrola-Vannucci, Ana K. et al.Characterization and functional analysis of the proteins Prohibitin 1 and 2 in Trypanosoma cruziPLOS Neglected Tropical Diseases2021ISSN 1935--2735
Dixon, Charles R. et al.STING nuclear partners contribute to innate immune signaling responsesiScience2021ISSN 2589-0042
Creighton, Blake A. et al.Giant ankyrin-B mediates transduction of axon guidance and collateral branch pruning factor sema 3AeLife2021ISSN 2050-084X
Lemjabbar-Alaoui, Hassan et al.AMXI-5001, a novel dual parp1/2 and microtubule polymerization inhibitor for the treatment of human cancers.American journal of cancer research2020ISSN 2156--6976
Yang, Yu et al.Identification of the repressive domain of the negative circadian clock component CHRONOInternational Journal of Molecular Sciences2020ISSN 1422-0067
Samson, Andre L. et al.MLKL trafficking and accumulation at the plasma membrane control the kinetics and threshold for necroptosisNature Communications2020ISSN 2041-1723
Batlle, Cristina et al.hnRNPDL Phase Separation Is Regulated by Alternative Splicing and Disease-Causing Mutations Accelerate Its AggregationCell Reports2020ISSN 2211-1247
Kumar Gaire, Sunil et al.Accelerating multicolor spectroscopic single-molecule localization microscopy using deep learningBiomedical Optics Express2020ISSN 2156--7085
Hirst, William G. et al.Differences in Intrinsic Tubulin Dynamic Properties Contribute to Spindle Length Control in Xenopus SpeciesCurrent Biology2020ISSN 1879-0445
Song, Ki Hee et al.Symmetrically dispersed spectroscopic single-molecule localization microscopyLight: Science and Applications2020ISSN 2047-7538
Even, Aviel et al.ATAT1-enriched vesicles promote microtubule acetylation via axonal transportScience Advances2019ISSN 2375-2548
Song, Ki-Hee et al.Three-dimensional biplane spectroscopic single-molecule localization microscopy: erratumOptica2019Article Link
Lie-Jensen, Anette et al.Centralspindlin Recruits ALIX to the Midbody during Cytokinetic Abscission in Drosophila via a Mechanism Analogous to Virus BuddingCurrent Biology2019ISSN 0960-9822
Fu, Meng meng et al.The Golgi Outpost Protein TPPP Nucleates Microtubules and Is Critical for MyelinationCell2019ISSN 1097-4172
Tejedo, Milvia Iris Alata et al.3,3′-Thiodipropanol As a Versatile Refractive Index-Matching Mounting Medium for Fluorescence MicroscopyBiomedical Optics Express2019ISSN 2156--7085
Sasaki, Takema et al.A Novel Katanin-Tethering Machinery Accelerates CytokinesisCurrent Biology2019ISSN 0960-9822
Schopfer, Lawrence M. et al.Chlorpyrifos oxon promotes tubulin aggregation via isopeptide cross-linking between diethoxyphospho-Lys and Glu or ASP: Implications for neurotoxicityJournal of Biological Chemistry2018ISSN 1083-351X
Xu, Xiaoxue et al.Probenecid disrupts a novel pannexin 1-collapsin response mediator protein 2 interaction and increases microtubule stabilityFrontiers in Cellular Neuroscience2018ISSN 1662-5102
Morelli, Giovanni et al.p27Kip1 Modulates Axonal Transport by Regulating α-Tubulin Acetyltransferase 1 StabilityCell Reports2018ISSN 2211-1247
Horita, Henrick et al.Identifying Regulatory Posttranslational Modifications of PD-L1: A Focus on MonoubiquitinatonNeoplasia (United States)2017ISSN 1476-5586
Lee, Yuh Ru Julie et al.The Mitotic Function of Augmin Is Dependent on Its Microtubule-Associated Protein Subunit EDE1 in Arabidopsis thalianaCurrent Biology2017ISSN 0960-9822
Winfree, Lilly M. et al.Protein phosphatase 4 coordinates glial membrane recruitment and phagocytic clearance of degenerating axons in DrosophilaCell Death and Disease2017ISSN 2041-4889
Stojkov, Darko et al.ROS and glutathionylation balance cytoskeletal dynamics in neutrophil extracellular trap formationJournal of Cell Biology2017ISSN 1540-8140
Tillberg, Paul W. et al.Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodiesNature Biotechnology2016ISSN 1546-1696
Zimmermann, Maike et al.Cyclin G2 promotes cell cycle arrest in breast cancer cells responding to fulvestrant and metformin and correlates with patient survivalCell Cycle2016ISSN 1551-4005
Ferraro, Francesco et al.Weibel-Palade body size modulates the adhesive activity of its von Willebrand Factor cargo in cultured endothelial cellsScientific Reports2016ISSN 2045-2322
Bouge, Anne Laure et al.Tau excess impairs mitosis and kinesin-5 function, leading to aneuploidy and cell deathDMM Disease Models and Mechanisms2016ISSN 1754-8411
Westmoreland, D. et al.Super-resolution microscopy as a potential approach to diagnosis of platelet granule disordersJournal of Thrombosis and Haemostasis2016ISSN 1538-7836
Zhang, Yu Shrike et al.Hybrid Microscopy: Enabling Inexpensive High-Performance Imaging through Combined Physical and Optical MagnificationsScientific Reports2016ISSN 2045-2322
Valenzuela-Iglesias, A. et al.Profilin1 regulates invadopodium maturation in human breast cancer cellsEuropean Journal of Cell Biology2015ISSN 1618-1298
McLane, Joshua S. et al.Palladin Mediates Stiffness-Induced Fibroblast Activation in the Tumor MicroenvironmentBiophysical Journal2015ISSN 1542-0086
Petrini, Stefania et al.Intracellular distribution of glutathionylated proteins in cultured dermal fibroblasts by immunofluorescenceMethods in Molecular Biology2015ISSN 1064-3745
Berruti, Giovanna et al.USP8/UBPy-regulated sorting and the development of sperm acrosome: The recruitment of METReproduction2015ISSN 1741-7899
Lepicard, Simon et al.A presynaptic role of microtubule-associated protein 1/Futsch in Drosophila: Regulation of active zone number and neurotransmitter releaseJournal of Neuroscience2014ISSN 1529-2401
Piroli, Gerardo G. et al.Identification of protein succination as a novel modification of tubulinBiochemical Journal2014ISSN 1470-8728
Zimmermann, Maike et al.Elevated cyclin G2 expression intersects with DNA damage checkpoint signaling and is required for a potent G2/M checkpoint arrest response to doxorubicinJournal of Biological Chemistry2012ISSN 0021-9258
Racedo, S. E. et al.Dynamic changes of the Golgi apparatus during bovine in vitro oocyte maturationReproduction2012ISSN 1470-1626
Ho, Chin Min Kimmy et al.Interaction of antiparallel microtubules in the phragmoplast is mediated by the microtubule-associated protein MAP65-3 in ArabidopsisThe Plant cell2011ISSN 1532--298X
Ho, Chin Min Kimmy et al.Augmin plays a critical role in organizing the spindle and phragmoplast microtubule arrays in ArabidopsisThe Plant cell2011ISSN 1532--298X
Davis, Ashley et al.A tubulin polymerization microassay used to compare ligand efficacyMethods in Cell Biology2010ISSN 0091-679X
Parrotta, Luigi et al.Changes in the accumulation of α- and β-tubulin during bud development in Vitis vinifera L.Planta2010ISSN 0032-0935
Jin, Li Qing et al.Axon regeneration in the absence of growth cones: Acceleration by cyclic AMPJournal of Comparative Neurology2009ISSN 0021-9967
Del Duca, Stefano et al.Effects of post-translational modifications catalysed by pollen transglutaminase on the functional properties of microtubulesand actin filamentsBiochemical Journal2009ISSN 1470-8728
Fujikake, Nobuhiro et al.Heat shock transcription factor 1-activating compounds suppress polyglutamine-induced neurodegeneration through induction of multiple molecular chaperonesThe Journal of biological chemistry2008ISSN 0021--9258
Van De Wetering, C. I. et al.Erratum: Chromosomal instability and supernumerary centrosomes represent precursor defects in a mouse model of T-cell lymphoma (Cancer Research (2007) 67, (8081-8088))Cancer Research2007ISSN 0008-5472
Semenova, Irina et al.Fluorescence microscopy of microtubules in cultured cells.Methods in molecular medicine2007ISSN 1543-1894
Hanson, Susan M. et al.Arrestin Mobilizes Signaling Proteins to the Cytoskeleton and Redirects their ActivityJournal of Molecular Biology2007ISSN 0022-2836
Ganot, Philippe et al.Comparative organization of follicle, accessory cells and spawning anlagen in dynamic semelparous clutch manipulators, the urochordate OikopleuridaeBiology of the Cell2006ISSN 0248-4900
Morito, Yuki et al.Dynamics of microtubules and positioning of female pronucleus during bovine parthenogenesisBiology of Reproduction2005ISSN 0006-3363
Sanbe, Atsushi et al.Desmin-related cardiomyopathy in transgenic mice: A cardiac amyloidosisProceedings of the National Academy of Sciences of the United States of America2004ISSN 0027-8424
Payne, Christopher et al.Preferentially localized dynein and perinuclear dynactin associate with nuclear pore complex proteins to mediate genomic union during mammalian fertilizationJournal of Cell Science2003ISSN 0021-9533

 

Question 1: What is the antigen that this antibody was raised against?

Answer 1:  The immunogen used for antibody production was a mixture of purified tubulins and therefore the polyclonal antibody has broad species cross reactivity from yeast to humans.

 

Question 2:  What species has this antibody been tested against?

Answer 2:  This polyclonal antibody is recommended for detection of tubulin in all yeast, fungi, and animal species including Drosophila melanogaster, Xenopus laevis, mouse, rat, human and bovine cell/tissue extracts. 

 

 

If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com