General tubulin buffer: 1x stock

General tubulin buffer: 1x stock
$0.00

Product Uses Include

  •  Resuspension and storage of tubulin

Material
General tubulin buffer (PEM). Contains 80 mM PIPES pH 6.9, 2 mM MgCl2 and 0.5 mM EGTA. Used as a tubulin working buffer in the form of G-PEM (i.e. PEM plus 1 mM GTP (Cat.# BST06)) or a tubulin polymerization buffer in the form of glycerol containing G-PEM (PEM plus 1 mM GTP (Cat. # BST06) and e.g. 5% glycerol (see Cat. # BST05)).

For product Datasheets and MSDSs please click on the PDF links below.   For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com

AuthorTitleJournalYearArticle Link
Lucas, Lathan et al.Alternatively Spliced MAP4 Isoforms Have Key Roles in Maintaining Microtubule Organization and Skeletal Muscle FunctioniScience2024
Singh, Sudhir K. et al.Noncanonical interaction with microtubules via the N-terminal nonmotor domain is critical for the functions of a bidirectional kinesinScience Advances2024
Hammarin, Greger et al.No observable non-thermal effect of microwave radiation on the growth of microtubulesScientific Reports2024
Singh, Rajendra K. et al.Coordinated Biophysical Stimulation of MSCs via Electromagnetized Au-Nanofiber Matrix Regulates Cytoskeletal-to-Nuclear Mechanoresponses and Lineage SpecificationAdvanced Functional Materials2023
Feizabadi, Mitra Shojania et al.The Effect of Tau and Taxol on Polymerization of MCF7 Microtubules In VitroInternational Journal of Molecular Sciences2022
Wu, R. et al.Thermal hysteresis in microtubule assembly/disassembly dynamics: The aging-induced degradation of tubulin dimersBiochemistry and Biophysics Reports2022
Pizzi, Rita et al.Evidences of new biophysical properties of microtubulesFocus on Artificial Neural Networks2021
Hough, Cameron M. et al.Disassembly of microtubules by intense terahertz pulsesBiomedical Optics Express2021
Li, Yaxin et al.Small-Molecule HSP27 Inhibitor Abolishes Androgen Receptors in GlioblastomaJournal of Medicinal Chemistry2021
Lin, Jong Wei et al.Dexamethasone accelerates muscle regeneration by modulating kinesin-1-mediated focal adhesion signalsCell Death Discovery2021
McElmurry, Kristi et al.Dynein-mediated microtubule translocation powering neurite outgrowth in chick and Aplysia neurons requires microtubule assemblyJournal of Cell Science2020
Tuszynski, Jack A. et al.Microtubules as Sub-Cellular MemristorsScientific Reports2020
Kalra, Aarat P. et al.Behavior of α, β tubulin in DMSO-containing electrolytesNanoscale Advances2019
Melo, Esther et al.HtrA1 Mediated Intracellular Effects on Tubulin Using a Polarized RPE Disease ModelEBioMedicine2018
Chen, Yang et al.Visualizing Autophagic Lysosome Reformation in Cells Using In Vitro Reconstitution SystemsCurrent Protocols in Cell Biology2018
Lukinavičius, Gražvydas et al.Fluorescent dyes and probes for super-resolution microscopy of microtubules and tracheoles in living cells and tissuesChemical Science2018
Mangiatordi, Giuseppe Felice et al.Automated identification of structurally heterogeneous and patentable antiproliferative hits as potential tubulin inhibitorsChemical Biology and Drug Design2018
Santelices, Iara B. et al.Response to Alternating Electric Fields of Tubulin Dimers and Microtubule Ensembles in Electrolytic SolutionsScientific Reports2017
Tantak, Mukund P. et al.Design and synthesis of bis(indolyl)ketohydrazide-hydrazones: Identification of potent and selective novel tubulin inhibitorsEuropean Journal of Medicinal Chemistry2017
Mangiatordi, Giuseppe Felice et al.Novel chemotypes targeting tubulin at the colch***** binding site and unbiasing P-glycoproteinEuropean Journal of Medicinal Chemistry2017
DeBruhl, Heather et al.Rop, the Sec1/Munc18 homolog in Drosophila, is required for furrow ingression and stable cell shape during cytokinesisJournal of Cell Science2016
Lee, Dongmin et al.Enhanced expression and purification of inositol 1,4,5-trisphosphate 3-kinase A through use of the pCold1-GST vector and a C-terminal hexahistidine tag in Escherichia coliProtein Expression and Purification2014
Hartley, R. M. et al.Polygamain, a New Microtubule Depolymerizing Agent That Occupies a Unique Pharmacophore in the Colch***** SiteMolecular Pharmacology2012
Chen, Chun Han et al.Protopine, a novel microtubule-stabilizing agent, causes mitotic arrest and apoptotic cell death in human hormone-refractory prostate cancer cell linesCancer Letters2012
Carletti, B. et al.Effect of protein glutathionylation on neuronal cytoskeleton: a potential link to neurodegenerationNeuroscience2011
Risinger, A. L. et al.ELR510444, a novel microtubule disruptor with multiple mechanisms of actionThe Journal of pharmacology and experimental therapeutics2011
Faridi, Uzma et al.Proteomics indicates modulation of tubulin polymerization by L-menthol inhibiting human epithelial colorectal adenocarcinoma cell proliferationProteomics2011
Chang, Chia Hao et al.Mycotoxin citrinin induced cell cycle G2/M arrest and numerical chromosomal aberration associated with disruption of microtubule formation in human cellsToxicological sciences : an official journal of the Society of Toxicology2011
Ratnikova, Tatsiana A. et al.In vitro polymerization of microtubules with a fullerene derivativeACS Nano2011
Kawaratani, Yasuyuki et al.New microtubule polymerization inhibitors comprising a nitrooxymethylphenyl groupBioorganic and Medicinal Chemistry2011
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Gutiérrez-Medina, Braulio et al.Visualizing individual microtubules by bright field microscopyAmerican Journal of Physics2010
Davis, Ashley et al.A tubulin polymerization microassay used to compare ligand efficacyMethods in Cell Biology2010
Kushkuley, Jacob et al.Neurofilament cross-bridging competes with kinesin-dependent association of neurofilaments with microtubulesJournal of cell science2009
Prager-Khoutorsky, Masha et al.Allicin inhibits cell polarization, migration and division via its direct effect on microtubulesCell Motility and the Cytoskeleton2007
Huang, Yao Ting et al.CIL-102 interacts with microtubule polymerization and causes mitotic arrest following apoptosis in the human prostate cancer PC-3 cell lineThe Journal of biological chemistry2005
Chen, Zehan et al.A-432411, a novel indolinone compound that disrupts spindle pole formation and inhibits human cancer cell growth2005

 

Question 1:  How does general tubulin buffer differ from G-PEM buffer?

Answer 1: Basically, these buffers are the same except that G-PEM contains 1mM GTP to support tubulin polymerization.  General tubulin buffer (Cat. # BST01) and PEM buffer typically contain 80 mM PIPES, 2 mM MgCl2, 0.5 mM EGTA pH 7.0.  Both buffers should be supplemented with 1 mM GTP (hence, the “G” in G-PEM buffer).

 

Question 2: Is glycerol necessary for the maintenance of biologically-active tubulin?

Answer 2: Glycerol is often added to a final concentration of 5 - 10% to enhance polymerization; however, glycerol is not necessary for the maintenance of biologically active tubulin.

 

 

If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com