General tubulin buffer: 1x stock

General tubulin buffer: 1x stock

Product Uses Include

  •  Resuspension and storage of tubulin

General tubulin buffer (PEM). Contains 80 mM PIPES pH 6.9, 2 mM MgCl2 and 0.5 mM EGTA. Used as a tubulin working buffer in the form of G-PEM (i.e. PEM plus 1 mM GTP (Cat.# BST06)) or a tubulin polymerization buffer in the form of glycerol containing G-PEM (PEM plus 1 mM GTP (Cat. # BST06) and e.g. 5% glycerol (see Cat. # BST05)).

For product Datasheets and MSDSs please click on the PDF links below.   For additional information, click on the FAQs tab above or contact our Technical Support department at

AuthorTitleJournalYearArticle Link
Wu, R. et al.Thermal hysteresis in microtubule assembly/disassembly dynamics: The aging-induced degradation of tubulin dimersBiochemistry and Biophysics Reports2022ISSN 2405-5808
Feizabadi, Mitra Shojania et al.The Effect of Tau and Taxol on Polymerization of MCF7 Microtubules In VitroInternational Journal of Molecular Sciences2022ISSN 1422-0067
Li, Yaxin et al.Small-Molecule HSP27 Inhibitor Abolishes Androgen Receptors in GlioblastomaJournal of Medicinal Chemistry2021ISSN 1520-4804
Lin, Jong Wei et al.Dexamethasone accelerates muscle regeneration by modulating kinesin-1-mediated focal adhesion signalsCell Death Discovery2021ISSN 2058-7716
Pizzi, Rita et al.Evidences of new biophysical properties of microtubulesFocus on Artificial Neural Networks2021Article Link
Hough, Cameron M. et al.Disassembly of microtubules by intense terahertz pulsesBiomedical Optics Express2021ISSN 2156--7085
Tuszynski, Jack A. et al.Microtubules as Sub-Cellular MemristorsScientific Reports2020ISSN 2045-2322
McElmurry, Kristi et al.Dynein-mediated microtubule translocation powering neurite outgrowth in chick and Aplysia neurons requires microtubule assemblyJournal of Cell Science2020ISSN 1477-9137
Kalra, Aarat P. et al.Behavior of α, β tubulin in DMSO-containing electrolytesNanoscale Advances2019ISSN 2516-0230
Mangiatordi, Giuseppe Felice et al.Automated identification of structurally heterogeneous and patentable antiproliferative hits as potential tubulin inhibitorsChemical Biology and Drug Design2018ISSN 1747-0285
Lukinavičius, Gražvydas et al.Fluorescent dyes and probes for super-resolution microscopy of microtubules and tracheoles in living cells and tissuesChemical Science2018ISSN 2041-6539
Chen, Yang et al.Visualizing Autophagic Lysosome Reformation in Cells Using In Vitro Reconstitution SystemsCurrent Protocols in Cell Biology2018ISSN 1934-2616
Melo, Esther et al.HtrA1 Mediated Intracellular Effects on Tubulin Using a Polarized RPE Disease ModelEBioMedicine2018ISSN 2352-3964
Tantak, Mukund P. et al.Design and synthesis of bis(indolyl)ketohydrazide-hydrazones: Identification of potent and selective novel tubulin inhibitorsEuropean Journal of Medicinal Chemistry2017ISSN 1768-3254
Santelices, Iara B. et al.Response to Alternating Electric Fields of Tubulin Dimers and Microtubule Ensembles in Electrolytic SolutionsScientific Reports2017ISSN 2045-2322
Mangiatordi, Giuseppe Felice et al.Novel chemotypes targeting tubulin at the colchicine binding site and unbiasing P-glycoproteinEuropean Journal of Medicinal Chemistry2017ISSN 1768-3254
DeBruhl, Heather et al.Rop, the Sec1/Munc18 homolog in Drosophila, is required for furrow ingression and stable cell shape during cytokinesisJournal of Cell Science2016ISSN 1477-9137
Lee, Dongmin et al.Enhanced expression and purification of inositol 1,4,5-trisphosphate 3-kinase A through use of the pCold1-GST vector and a C-terminal hexahistidine tag in Escherichia coliProtein Expression and Purification2014ISSN 1046-5928
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Chen, Chun Han et al.Protopine, a novel microtubule-stabilizing agent, causes mitotic arrest and apoptotic cell death in human hormone-refractory prostate cancer cell lines2012PMID 22033245
Carletti, B. et al.Effect of protein glutathionylation on neuronal cytoskeleton: a potential link to neurodegenerationNeuroscience2011ISSN 1873--7544
Kawaratani, Yasuyuki et al.New microtubule polymerization inhibitors comprising a nitrooxymethylphenyl groupBioorganic and Medicinal Chemistry2011ISSN 0968-0896
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Chang, Chia Hao et al.Mycotoxin citrinin induced cell cycle G2/M arrest and numerical chromosomal aberration associated with disruption of microtubule formation in human cellsToxicological sciences : an official journal of the Society of Toxicology2011ISSN 1096--0929
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Question 1:  How does general tubulin buffer differ from G-PEM buffer?

Answer 1: Basically, these buffers are the same except that G-PEM contains 1mM GTP to support tubulin polymerization.  General tubulin buffer (Cat. # BST01) and PEM buffer typically contain 80 mM PIPES, 2 mM MgCl2, 0.5 mM EGTA pH 7.0.  Both buffers should be supplemented with 1 mM GTP (hence, the “G” in G-PEM buffer).


Question 2: Is glycerol necessary for the maintenance of biologically-active tubulin?

Answer 2: Glycerol is often added to a final concentration of 5 - 10% to enhance polymerization; however, glycerol is not necessary for the maintenance of biologically active tubulin.



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