General tubulin buffer: 1x stock

General tubulin buffer: 1x stock
$0.00

Product Uses Include

  •  Resuspension and storage of tubulin

Material
General tubulin buffer (PEM). Contains 80 mM PIPES pH 6.9, 2 mM MgCl2 and 0.5 mM EGTA. Used as a tubulin working buffer in the form of G-PEM (i.e. PEM plus 1 mM GTP (Cat.# BST06)) or a tubulin polymerization buffer in the form of glycerol containing G-PEM (PEM plus 1 mM GTP (Cat. # BST06) and e.g. 5% glycerol (see Cat. # BST05)).

For product Datasheets and MSDSs please click on the PDF links below.   For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com

AuthorTitleJournalYearArticle Link
Singh, Rajendra K. et al.Coordinated Biophysical Stimulation of MSCs via Electromagnetized Au-Nanofiber Matrix Regulates Cytoskeletal-to-Nuclear Mechanoresponses and Lineage SpecificationAdvanced Functional Materials2023ISSN 1616--3028
Feizabadi, Mitra Shojania et al.The Effect of Tau and Taxol on Polymerization of MCF7 Microtubules In VitroInternational Journal of Molecular Sciences2022ISSN 1422-0067
Wu, R. et al.Thermal hysteresis in microtubule assembly/disassembly dynamics: The aging-induced degradation of tubulin dimersBiochemistry and Biophysics Reports2022ISSN 2405-5808
Pizzi, Rita et al.Evidences of new biophysical properties of microtubulesFocus on Artificial Neural Networks2021Article Link
Hough, Cameron M. et al.Disassembly of microtubules by intense terahertz pulsesBiomedical Optics Express2021ISSN 2156--7085
Li, Yaxin et al.Small-Molecule HSP27 Inhibitor Abolishes Androgen Receptors in GlioblastomaJournal of Medicinal Chemistry2021ISSN 1520-4804
Lin, Jong Wei et al.Dexamethasone accelerates muscle regeneration by modulating kinesin-1-mediated focal adhesion signalsCell Death Discovery2021ISSN 2058-7716
McElmurry, Kristi et al.Dynein-mediated microtubule translocation powering neurite outgrowth in chick and Aplysia neurons requires microtubule assemblyJournal of Cell Science2020ISSN 1477-9137
Tuszynski, Jack A. et al.Microtubules as Sub-Cellular MemristorsScientific Reports2020ISSN 2045-2322
Kalra, Aarat P. et al.Behavior of α, β tubulin in DMSO-containing electrolytesNanoscale Advances2019ISSN 2516-0230
Melo, Esther et al.HtrA1 Mediated Intracellular Effects on Tubulin Using a Polarized RPE Disease ModelEBioMedicine2018ISSN 2352-3964
Chen, Yang et al.Visualizing Autophagic Lysosome Reformation in Cells Using In Vitro Reconstitution SystemsCurrent Protocols in Cell Biology2018ISSN 1934-2616
Lukinavičius, Gražvydas et al.Fluorescent dyes and probes for super-resolution microscopy of microtubules and tracheoles in living cells and tissuesChemical Science2018ISSN 2041-6539
Mangiatordi, Giuseppe Felice et al.Automated identification of structurally heterogeneous and patentable antiproliferative hits as potential tubulin inhibitorsChemical Biology and Drug Design2018ISSN 1747-0285
Santelices, Iara B. et al.Response to Alternating Electric Fields of Tubulin Dimers and Microtubule Ensembles in Electrolytic SolutionsScientific Reports2017ISSN 2045-2322
Tantak, Mukund P. et al.Design and synthesis of bis(indolyl)ketohydrazide-hydrazones: Identification of potent and selective novel tubulin inhibitorsEuropean Journal of Medicinal Chemistry2017ISSN 1768-3254
Mangiatordi, Giuseppe Felice et al.Novel chemotypes targeting tubulin at the colchicine binding site and unbiasing P-glycoproteinEuropean Journal of Medicinal Chemistry2017ISSN 1768-3254
DeBruhl, Heather et al.Rop, the Sec1/Munc18 homolog in Drosophila, is required for furrow ingression and stable cell shape during cytokinesisJournal of Cell Science2016ISSN 1477-9137
Lee, Dongmin et al.Enhanced expression and purification of inositol 1,4,5-trisphosphate 3-kinase A through use of the pCold1-GST vector and a C-terminal hexahistidine tag in Escherichia coliProtein Expression and Purification2014ISSN 1046-5928
Hartley, R. M. et al.Polygamain, a New Microtubule Depolymerizing Agent That Occupies a Unique Pharmacophore in the Colchicine SiteMolecular Pharmacology2012ISSN 0026-895X
Chen, Chun Han et al.Protopine, a novel microtubule-stabilizing agent, causes mitotic arrest and apoptotic cell death in human hormone-refractory prostate cancer cell lines2012PMID 22033245
Carletti, B. et al.Effect of protein glutathionylation on neuronal cytoskeleton: a potential link to neurodegenerationNeuroscience2011ISSN 1873--7544
Risinger, A. L. et al.ELR510444, a novel microtubule disruptor with multiple mechanisms of actionThe Journal of pharmacology and experimental therapeutics2011ISSN 1521--0103
Faridi, Uzma et al.Proteomics indicates modulation of tubulin polymerization by L-menthol inhibiting human epithelial colorectal adenocarcinoma cell proliferation2011ISSN 1615--9861
Chang, Chia Hao et al.Mycotoxin citrinin induced cell cycle G2/M arrest and numerical chromosomal aberration associated with disruption of microtubule formation in human cellsToxicological sciences : an official journal of the Society of Toxicology2011ISSN 1096--0929
Ratnikova, Tatsiana A. et al.In vitro polymerization of microtubules with a fullerene derivativeACS Nano2011ISSN 1936-0851
Kawaratani, Yasuyuki et al.New microtubule polymerization inhibitors comprising a nitrooxymethylphenyl groupBioorganic and Medicinal Chemistry2011ISSN 0968-0896
O'Boyle, Niamh M. et al.Synthesis and evaluation of azetidinone analogues of combretastatin A-4 as tubulin targeting agentsJournal of Medicinal Chemistry2010ISSN 0022-2623
Gutiérrez-Medina, Braulio et al.Visualizing individual microtubules by bright field microscopyAmerican Journal of Physics2010ISSN 0002--9505
Davis, Ashley et al.A tubulin polymerization microassay used to compare ligand efficacyMethods in Cell Biology2010ISSN 0091-679X
Kushkuley, Jacob et al.Neurofilament cross-bridging competes with kinesin-dependent association of neurofilaments with microtubulesJournal of cell science2009ISSN 1477--9137
Prager-Khoutorsky, Masha et al.Allicin inhibits cell polarization, migration and division via its direct effect on microtubulesCell Motility and the Cytoskeleton2007ISSN 0886-1544
Huang, Yao Ting et al.CIL-102 interacts with microtubule polymerization and causes mitotic arrest following apoptosis in the human prostate cancer PC-3 cell lineThe Journal of biological chemistry2005ISSN 0021--9258
Chen, Zehan et al.A-432411, a novel indolinone compound that disrupts spindle pole formation and inhibits human cancer cell growth2005Article Link

 

Question 1:  How does general tubulin buffer differ from G-PEM buffer?

Answer 1: Basically, these buffers are the same except that G-PEM contains 1mM GTP to support tubulin polymerization.  General tubulin buffer (Cat. # BST01) and PEM buffer typically contain 80 mM PIPES, 2 mM MgCl2, 0.5 mM EGTA pH 7.0.  Both buffers should be supplemented with 1 mM GTP (hence, the “G” in G-PEM buffer).

 

Question 2: Is glycerol necessary for the maintenance of biologically-active tubulin?

Answer 2: Glycerol is often added to a final concentration of 5 - 10% to enhance polymerization; however, glycerol is not necessary for the maintenance of biologically active tubulin.

 

 

If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com