GTP: 100mM stock

GTP: 100mM stock

Product Uses Include 

  • Stabilise and promote polymerization of tubulin Substrate for GTPases


Guanosine triphosphate (GTP). 100 mM solution of GTP. Provided as a lyophilized powder. Required for tubulin stability and microtubule assembly and dynamics.

For product Datasheets and MSDSs please click on the PDF links below.   For additional information, click on the FAQs tab above or contact our Technical Support department at

AuthorTitleJournalYearArticle Link
Feizabadi, Mitra Shojania et al.The Effect of Tau and Taxol on Polymerization of MCF7 Microtubules In VitroInternational Journal of Molecular Sciences2022ISSN 1422-0067
Wu, R. et al.Thermal hysteresis in microtubule assembly/disassembly dynamics: The aging-induced degradation of tubulin dimersBiochemistry and Biophysics Reports2022ISSN 2405-5808
Hough, Cameron M. et al.Disassembly of microtubules by intense terahertz pulsesBiomedical Optics Express2021ISSN 2156--7085
Li, Yaxin et al.Small-Molecule HSP27 Inhibitor Abolishes Androgen Receptors in GlioblastomaJournal of Medicinal Chemistry2021ISSN 1520-4804
Qiu, Rongde et al.Dynein activation in vivo is regulated by the nucleotide states of its AAA3 domainCurrent Biology2021ISSN 1879-0445
Pizzi, Rita et al.Evidences of new biophysical properties of microtubulesFocus on Artificial Neural Networks2021Article Link
Kalra, Aarat P. et al.Investigation of the electrical properties of microtubule ensembles under cell-like conditionsNanomaterials2020ISSN 2079-4991
Kaur, Simranpreet et al.Expansion of the phenotypic spectrum of de novo missense variants in kinesin family member 1A (KIF1A)Human Mutation2020ISSN 1098-1004
Baker, Stacey J. et al.A Contaminant Impurity, Not Rigosertib, Is a Tubulin Binding AgentMolecular Cell2020ISSN 1097-4164
Budaitis, Breane G. et al.Neck linker docking is critical for kinesin-1 force generation in cells but at a cost to motor speed and processivityeLife2019ISSN 2050-084X
Bajaj, Rakhi et al.KNL1 Binding to PP1 and Microtubules Is Mutually ExclusiveStructure2018ISSN 1878-4186
Chen, Yang et al.Visualizing Autophagic Lysosome Reformation in Cells Using In Vitro Reconstitution SystemsCurrent Protocols in Cell Biology2018ISSN 1934-2616
Melo, Esther et al.HtrA1 Mediated Intracellular Effects on Tubulin Using a Polarized RPE Disease ModelEBioMedicine2018ISSN 2352-3964
Reinemann, Dana N. et al.Processive Kinesin-14 HSET Exhibits Directional Flexibility Depending on Motor TrafficCurrent Biology2018ISSN 0960-9822
Zhu, Yili et al.An in vitro Microscopy-based Assay for Microtubule-binding and Microtubule-crosslinking by Budding Yeast Microtubule-associated ProteinBio-Protocol2018ISSN 2331--8325
Reinemann, Dana N. et al.Collective Force Regulation in Anti-parallel Microtubule Gliding by Dimeric Kif15 Kinesin MotorsCurrent Biology2017ISSN 0960-9822
Su, Qian Peter et al.Corrigendum: Vesicle Size Regulates Nanotube Formation in the CellScientific reports2017ISSN 2045-2322
Du, Wanqing et al.Kinesin 1 Drives Autolysosome TubulationDevelopmental Cell2016ISSN 1878-1551
Wang, Chong et al.Dynamic tubulation of mitochondria drives mitochondrial network formationCell Research2015ISSN 1748-7838
Makrantoni, Vasso et al.Phosphorylation of Sli15 by Ipl1 is important for proper CPC localization and chromosome stability in Saccharomyces cerevisiaePLoS ONE2014ISSN 1932-6203
Lee, Dongmin et al.Enhanced expression and purification of inositol 1,4,5-trisphosphate 3-kinase A through use of the pCold1-GST vector and a C-terminal hexahistidine tag in Escherichia coliProtein Expression and Purification2014ISSN 1046-5928
Xiao, Xiang et al.c-Yes regulates cell adhesion at the apical ectoplasmic specialization-blood-testis barrier axis via its effects on protein recruitment and distribution2013PMID 23169788
Lee, D et al.Inositol 1, 4, 5-Trisphosphate 3-Kinase A Is a Novel Microtubule-associated ProteinJournal of Biological …2012Article Link
Hartley, R. M. et al.Polygamain, a New Microtubule Depolymerizing Agent That Occupies a Unique Pharmacophore in the Colchicine SiteMolecular Pharmacology2012ISSN 0026-895X
Butler, Suzanne C. et al.Inhibitory effects of pectenotoxins from marine algae on the polymerization of various actin isoformsToxicology in Vitro2012ISSN 0887-2333
Chen, Chun Han et al.Protopine, a novel microtubule-stabilizing agent, causes mitotic arrest and apoptotic cell death in human hormone-refractory prostate cancer cell lines2012PMID 22033245
Faridi, Uzma et al.Proteomics indicates modulation of tubulin polymerization by L-menthol inhibiting human epithelial colorectal adenocarcinoma cell proliferation2011ISSN 1615--9861
Risinger, A. L. et al.ELR510444, a novel microtubule disruptor with multiple mechanisms of actionThe Journal of pharmacology and experimental therapeutics2011ISSN 1521--0103
Chang, Chia Hao et al.Mycotoxin citrinin induced cell cycle G2/M arrest and numerical chromosomal aberration associated with disruption of microtubule formation in human cellsToxicological sciences : an official journal of the Society of Toxicology2011ISSN 1096--0929
Ratnikova, Tatsiana A. et al.In vitro polymerization of microtubules with a fullerene derivativeACS Nano2011ISSN 1936-0851
Dempsey, Graham T. et al.Evaluation of fluorophores for optimal performance in localization-based super-resolution imagingNature Methods2011ISSN 1548-7091
Carletti, B. et al.Effect of protein glutathionylation on neuronal cytoskeleton: a potential link to neurodegenerationNeuroscience2011ISSN 1873--7544
Kawaratani, Yasuyuki et al.New microtubule polymerization inhibitors comprising a nitrooxymethylphenyl groupBioorganic and Medicinal Chemistry2011ISSN 0968-0896
Gutiérrez-Medina, Braulio et al.Visualizing individual microtubules by bright field microscopyAmerican Journal of Physics2010ISSN 0002--9505
O'Boyle, Niamh M. et al.Synthesis and evaluation of azetidinone analogues of combretastatin A-4 as tubulin targeting agentsJournal of Medicinal Chemistry2010ISSN 0022-2623
Davis, Ashley et al.A tubulin polymerization microassay used to compare ligand efficacyMethods in Cell Biology2010ISSN 0091-679X
Kushkuley, Jacob et al.Neurofilament cross-bridging competes with kinesin-dependent association of neurofilaments with microtubulesJournal of cell science2009ISSN 1477--9137
Huang, Yao Ting et al.CIL-102 interacts with microtubule polymerization and causes mitotic arrest following apoptosis in the human prostate cancer PC-3 cell lineThe Journal of biological chemistry2005ISSN 0021--9258
Chen, Zehan et al.A-432411, a novel indolinone compound that disrupts spindle pole formation and inhibits human cancer cell growth2005Article Link


Question 1: Does GTP need to be included in tubulin buffers prepared for reconstituting and storing tubulin if the tubulin will not be used for polymerization?

Answer 1:  Cytoskeleton’s tubulin proteins are lyophilized in a buffer that contains 1 mM GTP, so omitting additional GTP (Cat. # BST06) in the reconstitution buffer can be done if the tubulin will not be used for polymerization.  However, this is not recommended.  Under most preparation conditions, GTP is required for tubulin stability and for the formation of microtubules in vivo and in vitro and we recommend adding fresh GTP (1 mM) to the PEM buffer used for reconstitution and polymerization.


Question 2: How long is GTP-containing tubulin buffer stable for when stored on ice the day of the experiment?

Answer 2: Buffers that contain GTP (Cat. # BST06) should not be kept longer than 2 hours on ice.  After this time, GTP-supplemented buffers should be discarded. Do not attempt to freeze and re-use these buffers. 


If you have any questions concerning this product, please contact our Technical Service department at