Product Uses Include
Paclitaxel's (Taxol) usefulness as a laboratory tool is well established and lies in its ability to inhibit microtubule depolymerization. Thus, taxol stabilized microtubules are used as substrates for the identification and characterization of the ever increasing number of microtubule associated proteins.
Paclitaxel is provided as a lyophilized powder. When resuspended in DMSO (not included) the paclitaxel is at 2 mM concentration.
In keeping with our policy of providing only the highest quality products, we are now pleased to inform you that Cytoskeleton is offering paclitaxel (taxol), from the Pacific yew tree, Taxus brevifolia, at a chromatographic purity of >99.5%. This is the purest taxol commercially available for research use.
We have determined that the microtubule stabilizing property of our product is equal or superior to any other commercially available taxols. Microtubules in 10 µM paclitaxel are stable at room temperature for over one week.
Kosturko, L. D., Maggipinto, M. J., D'Sa, C., Carson, J. H. and Barbarese, E. (2005). The microtubule-associated protein tumor overexpressed gene binds to the RNA trafficking protein heterogeneous nuclear ribonucleoprotein A2. Mol. Biol. Cell 16, 1938-1947.
Teckchandani, A. M., Birukova, A. A., Tar, K., Verin, A. D. and Tsygankov, A. Y. (2005). The multidomain protooncogenic protein c-Cbl binds to tubulin and stabilizes microtubules. Exp. Cell Res. 306, 114-127.
Nair, K. S., Hanson, S. M., Kennedy, M. J., Hurley, J. B., Gurevich, V. V. and Slepak, V. Z. (2004). Direct binding of visual arrestin to microtubules determines the differential subcellular localization of its splice variants in rod photoreceptors. J. Biol. Chem. 279, 41240-41248.
Wagner, O. I., Ascano, J., Tokito, M., Leterrier, J. F., Janmey, P. A. and Holzbaur, E. L. (2004). The interaction of neurofilaments with the microtubule motor cytoplasmic dynein. Mol. Biol. Cell 15, 5092-5100
Ligon, L. A., Shelly, S. S., Tokito, M. and Holzbaur, E. L. (2003). The microtubule plus-end proteins EB1 and dynactin have differential effects on microtubule polymerization. Mol. Biol. Cell 14, 1405-1417.
Benink, H. A., Mandato, C. A. and Bement, W. M. (2000). Analysis of cortical flow models in vivo. Mol. Biol. Cell 11, 2553-2563.
Korinek, W. S., Copeland, M. J., Chaudhuri, A. and Chant, J. (2000). Molecular linkage underlying microtubule orientation toward cortical sites in yeast. Science 287, 2257-2259.
Question 1: Can I mix taxol with my cell culture media and store at 4°C?
Answer 1: No, we do not recommend storing cell culture media with taxol (Cat. # TXD01). The taxol could precipitate out of solution at 4°C since the taxol is prepared in a 100% DMSO solution. Instead, add taxol to warm cell culture media to be used on the day of the experiment.
Question 2: Can I incubate my cells with taxol to affect in vivo tubulin polymerization?
Answer 2: In-house testing demonstrates that 1 hour with 0.5 or 1 mM taxol (Cat. # TXD01) produces a robust increase in microtubules in Swiss 3T3 fibroblast cells.
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