Paclitaxel (Taxol)

Paclitaxel (Taxol)
$0.00

Product Uses Include

  • Promote tubulin polymerization in vitro
  • Promote microtubule stability in vitro
  • Anti-mitotic drug in cell cultures

Material
Paclitaxel's (Taxol) usefulness as a laboratory tool is well established and lies in its ability to inhibit microtubule depolymerization. Thus, taxol stabilized microtubules are used as substrates for the identification and characterization of the ever increasing number of microtubule associated proteins.

Paclitaxel is provided as a lyophilized powder. When resuspended in DMSO (not included) the paclitaxel is at 2 mM concentration.

Purity
In keeping with our policy of providing only the highest quality products, we are now pleased to inform you that Cytoskeleton is offering paclitaxel (taxol), from the Pacific yew tree, Taxus brevifolia, at a chromatographic purity of >99.5%. This is the purest taxol commercially available for research use.

Biological Activity
We have determined that the microtubule stabilizing property of our product is equal or superior to any other commercially available taxols. Microtubules in 10 µM paclitaxel are stable at room temperature for over one week.

For product Datasheets and MSDSs please click on the PDF links below.   For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com

Sinclair, A. et al. The Trypanosoma brucei subpellicular microtubule array is organized into functionally discrete subdomains defined by microtubule associated proteins https://doi.org/10.1371/journal.ppat.1009588

Kosturko, L. D., Maggipinto, M. J., D'Sa, C., Carson, J. H. and Barbarese, E. (2005). The microtubule-associated protein tumor overexpressed gene binds to the RNA trafficking protein heterogeneous nuclear ribonucleoprotein A2. Mol. Biol. Cell 16, 1938-1947.

Teckchandani, A. M., Birukova, A. A., Tar, K., Verin, A. D. and Tsygankov, A. Y. (2005). The multidomain protooncogenic protein c-Cbl binds to tubulin and stabilizes microtubules. Exp. Cell Res. 306, 114-127.

Nair, K. S., Hanson, S. M., Kennedy, M. J., Hurley, J. B., Gurevich, V. V. and Slepak, V. Z. (2004). Direct binding of visual arrestin to microtubules determines the differential subcellular localization of its splice variants in rod photoreceptors. J. Biol. Chem. 279, 41240-41248.

Wagner, O. I., Ascano, J., Tokito, M., Leterrier, J. F., Janmey, P. A. and Holzbaur, E. L. (2004). The interaction of neurofilaments with the microtubule motor cytoplasmic dynein. Mol. Biol. Cell 15, 5092-5100

Ligon, L. A., Shelly, S. S., Tokito, M. and Holzbaur, E. L. (2003). The microtubule plus-end proteins EB1 and dynactin have differential effects on microtubule polymerization. Mol. Biol. Cell 14, 1405-1417.

Benink, H. A., Mandato, C. A. and Bement, W. M. (2000). Analysis of cortical flow models in vivo. Mol. Biol. Cell 11, 2553-2563.

Korinek, W. S., Copeland, M. J., Chaudhuri, A. and Chant, J. (2000). Molecular linkage underlying microtubule orientation toward cortical sites in yeast. Science 287, 2257-2259.

AuthorTitleJournalYearArticle Link
Feizabadi, Mitra Shojania et al.The Effect of Tau and Taxol on Polymerization of MCF7 Microtubules In VitroInternational Journal of Molecular Sciences2022ISSN 1422-0067
Guerra San Juan, Irune et al.Loss of mouse Stmn2 function causes motor neuropathyNeuron2022ISSN 0896-6273
Gibson, James M et al.Coil-to-α-helix transition at the Nup358-BicD2 interface activates BicD2 for dynein recruitmenteLife2022ISSN 2050-084X
Qiu, Rongde et al.Dynein activation in vivo is regulated by the nucleotide states of its AAA3 domainCurrent Biology2021ISSN 1879-0445
Pizzi, Rita et al.Evidences of new biophysical properties of microtubulesFocus on Artificial Neural Networks2021Article Link
Chang, Ting Yu et al.A novel histone deacetylase inhibitor MPT0L184 dysregulates cell-cycle checkpoints and initiates unscheduled mitotic signalingBiomedicine and Pharmacotherapy2021ISSN 1950-6007
Pizzi, Rita et al.Evidences of new biophysical properties of microtubulesFocus on Artificial Neural Networks2021Article Link
Habicht, Juri et al.UNC-45A breaks the microtubule lattice independently of its effects on non-muscle myosin IIJournal of Cell Science2021ISSN 1477-9137
Li, Faxiang et al.Cryo-em structure of vash1-svbp bound to microtubuleseLife2020ISSN 2050-084X
Kalra, Aarat P. et al.Investigation of the electrical properties of microtubule ensembles under cell-like conditionsNanomaterials2020ISSN 2079-4991
Kaur, Simranpreet et al.Expansion of the phenotypic spectrum of de novo missense variants in kinesin family member 1A (KIF1A)Human Mutation2020ISSN 1098-1004
Chiolerio, Alessandro et al.On resistance switching and oscillations in tubulin microtubule dropletsJournal of Colloid and Interface Science2020ISSN 1095-7103
Hao, Huiwen et al.Golgi‐associated microtubules are fast cargo tracks and required for persistent cell migrationEMBO reports2020ISSN 1469--221X
Ait-Bouziad, Nadine et al.Phosphorylation of the overlooked tyrosine 310 regulates the structure, aggregation, and microtubule- And lipid-binding properties of TauJournal of Biological Chemistry2020ISSN 1083-351X
Budaitis, Breane G. et al.Neck linker docking is critical for kinesin-1 force generation in cells but at a cost to motor speed and processivityeLife2019ISSN 2050-084X
Guedes-Dias, Pedro et al.Kinesin-3 Responds to Local Microtubule Dynamics to Target Synaptic Cargo Delivery to the PresynapseCurrent Biology2019ISSN 0960-9822
Li, Feiran et al.Local direction change of surface gliding microtubulesBiotechnology and Bioengineering2019ISSN 1097-0290
Tjioe, Marco et al.Multiple kinesins induce tension for smooth cargo transporteLife2019ISSN 2050-084X
Huang, Chao et al.FoxM1 Induced Paclitaxel Resistance via Activation of the FoxM1/PHB1/RAF-MEK-ERK Pathway and Enhancement of the ABCA2 TransporterMolecular Therapy - Oncolytics2019ISSN 2372-7705
Schimert, Kristin I. et al.Intracellular cargo transport by single-headed kinesin motorsProceedings of the National Academy of Sciences of the United States of America2019ISSN 1091-6490
Zhou, Chen et al.Structural basis of tubulin detyrosination by VASH2/SVBP heterodimerNature Communications2019ISSN 2041-1723
Melo, Esther et al.HtrA1 Mediated Intracellular Effects on Tubulin Using a Polarized RPE Disease ModelEBioMedicine2018ISSN 2352-3964
Reinemann, Dana N. et al.Processive Kinesin-14 HSET Exhibits Directional Flexibility Depending on Motor TrafficCurrent Biology2018ISSN 0960-9822
Bajaj, Rakhi et al.KNL1 Binding to PP1 and Microtubules Is Mutually ExclusiveStructure2018ISSN 1878-4186
Chen, Yang et al.Visualizing Autophagic Lysosome Reformation in Cells Using In Vitro Reconstitution SystemsCurrent Protocols in Cell Biology2018ISSN 1934-2616
Cloer, E. W. et al.p62-Dependent Phase Separation of Patient-Derived KEAP1 Mutations and NRF2Molecular and Cellular Biology2018ISSN 0270--7306
Zhu, Yili et al.An in vitro Microscopy-based Assay for Microtubule-binding and Microtubule-crosslinking by Budding Yeast Microtubule-associated ProteinBio-Protocol2018ISSN 2331--8325
Qiang, Liang et al.Tau Does Not Stabilize Axonal Microtubules but Rather Enables Them to Have Long Labile DomainsCurrent Biology2018ISSN 0960-9822
Ganguly, Anindya et al.Importin-β Directly Regulates the Motor Activity and Turnover of a Kinesin-4Developmental Cell2018ISSN 1878-1551
Sladewski, Thomas E. et al.Recruitment of two dyneins to an mRNA-dependent bicaudal D transport complexeLife2018ISSN 2050-084X
McIntosh, Betsy B. et al.Opposing Kinesin and Myosin-I Motors Drive Membrane Deformation and Tubulation along Engineered Cytoskeletal NetworksCurrent Biology2018ISSN 0960-9822
Howes, Stuart C. et al.Structural differences between yeast and mammalian microtubules revealed by cryo-EMJournal of Cell Biology2017ISSN 1540-8140
Su, Qian Peter et al.Corrigendum: Vesicle Size Regulates Nanotube Formation in the CellScientific reports2017ISSN 2045-2322
Zollo, Massimo et al.PRUNE is crucial for normal brain development and mutated in microcephaly with neurodevelopmental impairmentBrain2017ISSN 1460-2156
Kandel, Mikhail E. et al.Label-Free Imaging of Single Microtubule Dynamics Using Spatial Light Interference MicroscopyACS Nano2017ISSN 1936-086X
Su, Qian Peter et al.Corrigendum: Vesicle Size Regulates Nanotube Formation in the CellScientific reports2017ISSN 2045-2322
Reinemann, Dana N. et al.Collective Force Regulation in Anti-parallel Microtubule Gliding by Dimeric Kif15 Kinesin MotorsCurrent Biology2017ISSN 0960-9822
Du, Wanqing et al.Kinesin 1 Drives Autolysosome TubulationDevelopmental Cell2016ISSN 1878-1551
Kellogg, Elizabeth H. et al.Near-atomic cryo-EM structure of PRC1 bound to the microtubuleProceedings of the National Academy of Sciences of the United States of America2016ISSN 1091-6490
Wang, Chong et al.Dynamic tubulation of mitochondria drives mitochondrial network formationCell Research2015ISSN 1748-7838
Matsuyama, Tomonori et al.Midazolam inhibits the hypoxia-induced up-regulation of erythropoietin in the central nervous systemEuropean Journal of Pharmacology2015ISSN 1879-0712
Makrantoni, Vasso et al.Phosphorylation of Sli15 by Ipl1 is important for proper CPC localization and chromosome stability in Saccharomyces cerevisiaePLoS ONE2014ISSN 1932-6203
Szyk, Agnieszka et al.Molecular basis for age-dependent microtubule acetylation by tubulin acetyltransferaseCell2014ISSN 1097-4172
Kadakkuzha, Beena M. et al.High-throughput screening for small molecule modulators of motor protein kinesinAssay and Drug Development Technologies2014ISSN 1557-8127
Batalla Mayoral, J. et al.Potencial zeta en la determinación de carga superficial de liposomas.Latin-American Journal of Physics Education2014ISSN 1870--9095
Lee, Dongmin et al.Enhanced expression and purification of inositol 1,4,5-trisphosphate 3-kinase A through use of the pCold1-GST vector and a C-terminal hexahistidine tag in Escherichia coliProtein Expression and Purification2014ISSN 1046-5928
Howes, Stuart C. et al.Effects of tubulin acetylation and tubulin acetyltransferase binding on microtubule structureMolecular Biology of the Cell2014ISSN 1059-1524
Hernández Candia, Carmen Noemí et al.A Minimal Optical Trapping and Imaging Microscopy SystemPLoS ONE2013ISSN 1932-6203
Lee, D et al.Inositol 1, 4, 5-Trisphosphate 3-Kinase A Is a Novel Microtubule-associated ProteinJournal of Biological …2012Article Link
Dempsey, Graham T. et al.Evaluation of fluorophores for optimal performance in localization-based super-resolution imagingNature Methods2011ISSN 1548-7091
Gutiérrez-Medina, Braulio et al.Visualizing individual microtubules by bright field microscopyAmerican Journal of Physics2010ISSN 0002--9505

 

Question 1: Can I mix taxol with my cell culture media and store at 4°C?

Answer 1: No, we do not recommend storing cell culture media with taxol (Cat. # TXD01).  The taxol could precipitate out of solution at 4°C since the taxol is prepared in a 100% DMSO solution.  Instead, add taxol to warm cell culture media to be used on the day of the experiment.

 

Question 2: Can I incubate my cells with taxol to affect in vivo tubulin polymerization?

Answer 2: In-house testing demonstrates that 1 hour with 0.5 or 1 mM taxol (Cat. # TXD01) produces a robust increase in microtubules in Swiss 3T3 fibroblast cells.

 

 

If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com