• Detect endogenous SUMO2/3 changes on a protein of interest (POI) in cells or tissue extracts
• Isolate the total SUMO2/3 modified protein profile from cells or tissues via immunoprecipitation (IP) for downstream proteomic analysis
• Confirm POI SUMOylation data generated from mass spec proteomics

SUMO2/3 proteins are small ubiquitin-like modifiers that reversibly attach to target proteins, influencing their stability, localization, and activity. These modifications are critical for regulating stress responses, DNA repair, and signal transduction pathways in eukaryotic cells.

ASM24-beads are an affinity reagent comprising a high-affinity/specificity pan-SUMO2/3 antibody ASM24 covalently linked to agarose beads.

Key characteristics

• Broad enrichment of SUMO2/3 modified proteins
• No heavy/light chain leaching during IP
• Sufficient reagent for 20 IP assays (1 mg lysate per IP)
• Consistent lot-to-lot performance
• Supplied as a lyophilized powder
• Buffer composition after rehydration: 200mM PIPES pH 7.4, 5% sucrose, 50% glycerol, and 1% dextran

The biological activity of ASM24-beads is demonstrated by the ability of the reagent to enrich a broad range of SUMO2/3-modified proteins from Swiss 3T3 cell lysates (40 µl beads: 1mg lysate). Specificity is demonstrated by the 5 fold increase in SUMO2/3 signal in heat shock treated lysates and 3 fold reduction in SUMO2/3 signal in shRNA SUMO-2 knockdown lysates (see data sheet).