Validated for:

• Western blot-WB
• Immunofluorescence-IF

Anti-actin antibody (AAN02) was raised against purified actin protein from rabbit skeletal muscle. It detects −skeletal, −cardiac, −smooth muscle, −cytoplasmic, − smooth muscle and − cytoplasmic actin isotypes. AAN02 is purified by Protein G affinity chromatography.

Host/isotype/clone:

• Mouse/IgG2b
• Clone: 7A8.2.1

Reactivity:

• Broad cross species reactivity

Amount of material:

• AAN02: 1 x 500 µl when reconstituted (40-60 µg per tube)
• AAN02-S: 1 x 125 µl when reconstituted (10-15 µg per tube)

Working concentrations:

• WB 1:1000 dilution 0.08 – 0.12 µg/ml final 500 ml working volume - full size Ab
• IF 1:500 dilution 0.16-0.24 µg/ml final 250 ml working volume - full size Ab

Formulation

• Supplied as a lyophilized powder
• Buffer composition after reconstitution in 50% glycerol in water: 200 mM PIPES pH7.0, 5% sucrose, 1% dextran, 50% glycerol

Sensitivity:

• Detects as little as 5 ng purified rabbit muscle actin.
• In native samples, a 43 kD actin band is clearly visible in 1 µg platelet extract

Specificity:

• Across a panel of cell lysates analyzed via Western blotting, the only detectable band is at ~43 kDa, corresponding to β- & γ actin.
• Under the recommended fixation protocols (see data sheet), actin filament structures (F-actin) are clearly revealed, with minimal to no staining of other cellular structures.