GTPase Activation Screening Services

Cyto’s proprietary G-LISA® (GTPase-Linked Immunosorbent Assay) ELISA-based platform-now available as a CRO fee-for-service-offers rapid, quantitative analysis of small G-protein activation profiles. Backed by scientists with deep expertise in cytoskeletal signaling and the G-LISA® platform, we deliver precision data you can trust.

Service overview

Small GTPases of the Ras superfamily are key drug targets in cancer and other diseases. Patented G-LISA® technology, developed by Cytoskeleton, quantifies GTPase activation in cell or tissue extracts. Ideal for low- to medium-throughput screening (10–1000 compounds), it's perfect for target validation and mechanistic studies. Now available as a fee-for-service:

You send: Lysates (or we culture & treat per your SOP).
We run: G-LISA on selected GTPases (Ras, RhoA, Rac1, Cdc42, etc.)
You receive: A full report including raw and normalized data.

GTPase targets

Target	Synonym	Datasheet	Inquire
Pan-Ras	Colorimetric or luminescence	CDS225	Custom Services Inquiry
K-Ras	Colorimetric or luminescence	CDS222	Custom Services Inquiry
RhoA	Colorimetric or luminescence	CDS228	Custom Services Inquiry
Rac1	Colorimetric or luminescence	CDS226	Custom Services Inquiry
Cdc42	Colorimetric	CDS221	Custom Services Inquiry
RalA	Colorimetric	CDS227	Custom Services Inquiry
Arf1	Colorimetric	Discontinued	Custom Services Inquiry
Other	Colorimetric Or Luminescence	CDS229	Custom Services Inquiry

Why choose us?

Cytoskeleton partners closely with your team to deliver custom assays, leveraging deep expertise in . Our high-quality data supports key project decisions, while our focus on collaboration and reliability keeps leading pharma and biotech companies returning year after year.

Proven expertise

Cyto developed and patented this technology
Run by trained scientists with deep expertise in small G-protein biochemistry
Experienced in experimental design to data analysis and reporting

Collaborative approach

Client priorities consistently drive the agenda.
Clients retain full flexibility to change the project direction at any stage
For longer projects, weekly meetings with both the PhD project lead and a dedicated project manager

Fast reliable delivery

Turn around for new projects as short as 1-2 weeks
The final report will be shaped in partnership with the client, incorporating their insights and approvals at key milestones

Reliable, robust results

Low variability ensures highly reproducible data
Rigorous, multi-parameter, QC protocol
Detailed documentation

Case Study

GEFs in Focus: Rac1 Activation

The objective

The client aimed to screen a library of 120 GEF genes for their effects on a GTPase linked to a human genetic defect. The objective was to transfect plasmid DNA into a cell line that modeled the genetic defect, confirm expression after 24 hours, and then prepare lysates to assess Rac1 GTPase activation. A fast turnaround was essential to complete transfection, expression validation, and Rac1 activity analysis across all genes.

The result

We completed high-throughput transfection of 120 GEF gene plasmids into the target cell model representing the genetic defect. Expression was confirmed for >95% of constructs, and lysates were prepared and analyzed efficiently. The activation profiles of the Rac1 GTPase were reliably quantified, enabling the identification of key GEFs that impact Rac1 activity. The rapid turnaround met the client’s timeline, delivering high-quality, actionable data for downstream analysis.

Download case study

Our clients

We proudly support leading research teams across pharma, biotech, and academia. Our custom services have helped advance discovery and preclinical programs in oncology, neuroscience, cardiology, and beyond.

Merck & Co., Inc.
Eli Lilly & Co.
Amgen, Inc.
Abbott Laboratories
Pfizer, Inc.
Astra-Zeneca plc
GlaxoSmithKline plc
Genentech, Inc.
Johnson & Johnson
Bristol-Myers Squibb

Let’s discuss how our G-LISA service can advance your next milestone

Service overview

You send: Lysates (or we culture & treat per your SOP).
We run: G-LISA on selected GTPases (Ras, RhoA, Rac1, Cdc42, etc.)
You receive: A full report including raw and normalized data.

Target

Synonym

Datasheet

Inquire

Pan-Ras

Colorimetric or luminescence

CDS225

Custom Services Inquiry

K-Ras

Colorimetric or luminescence

CDS222

Custom Services Inquiry

RhoA

Colorimetric or luminescence

CDS228

Custom Services Inquiry

Rac1

Colorimetric or luminescence

CDS226

Custom Services Inquiry

Cdc42

Colorimetric

CDS221

Custom Services Inquiry

RalA

Colorimetric

CDS227

Custom Services Inquiry

Arf1

Colorimetric

Discontinued

Custom Services Inquiry

Other

Colorimetric Or Luminescence

CDS229

Custom Services Inquiry

Why choose us?

Proven expertise

Cyto developed and patented this technology
Run by trained scientists with deep expertise in small G-protein biochemistry
Experienced in experimental design to data analysis and reporting

Collaborative approach

Client priorities consistently drive the agenda.
Clients retain full flexibility to change the project direction at any stage
For longer projects, weekly meetings with both the PhD project lead and a dedicated project manager

Fast reliable delivery

Turn around for new projects as short as 1-2 weeks
The final report will be shaped in partnership with the client, incorporating their insights and approvals at key milestones

Reliable, robust results

Low variability ensures highly reproducible data
Rigorous, multi-parameter, QC protocol
Detailed documentation