About Acti-stain
The Acti-stain™ line of fluorescent phalloidins has been developed with an emphasis on creating exceptionally bright and stable probes at an economical price. Side-by-side comparisons with leading competing products demonstrate that you will enjoy savings while not sacrificing the quality of the stain when switching to an Acti-stain™ probe. Additionally, these probes are compatible with popular filter sets such as FITC, TRITC and Cy5. The combination of in-house manufacturing, stringent quality control and convenient packaging provides a great value. Give them a try and see for yourself.

Product Uses Include

  • Stain F-actin in fixed cells
  • Stabilize actin filaments in vitro
  • Visualize actin filaments in vitro

 

    Actin staining is very useful in determining the structure and function of the cytoskeleton in living and fixed cells. The actin cytoskeleton is a very dynamic and labile structure in the living cell, but it can be fixed with paraformaldehyde prior to probing or staining for actin structures. 

    Acti-stain™ 488 phalloidin
    Cat. # PHDG1
    Acti-stain™ 555 phalloidin
    Cat. # PHDH1
    Acti-stain™ 670 phalloidin
    Cat. # PHDN1
    Swiss 3T3 cell stained with anti-vinculin (red), Dapi (blue nucleus) and F-actin is stained with Acti-stain™ 488 (green F-actin, Cat.# PHDG1). Swiss 3T3 cell with activated Rho, nucleus is stained with Dapi (blue) and F-actin is stained with Acti-stain™ 555 (red F-actin, Cat.# PHDH1). Mitotic Swiss 3T3 cell, F-actin stained with Acti-stain™ 670 (far-red, Cat. # PHDN1), nuclear DNA stained with Dapi (blue).

     

     

    Specifications:

    Figure 2. Emission and excitation scans for Acti-stain™ phalloidins

    2 4 6

    Absorbance and fluorescence scan of Acti-stain™ 488. Labeled phalloidin was diluted into methanol and its absorbance and excitation spectra were scanned between 350-750 and 500-750 nm, respectively. Absorbance peaks at 500 nm and fluorescence at 550 nm.

    Absorbance and fluorescence scan of Acti-stain™ 555. Labeled phalloidin was diluted into methanol and its absorbance and excitation spectra were scanned between 300-750 and 560-750 nm, respectively. Absorbance peaks at 560 nm and fluorescence at 575 nm.

    Absorbance and fluorescence scan of Acti-stain™ 670. Labeled phalloidin was diluted into methanol and its absorbance and excitation spectra were scanned between 300-750 and 600-750 nm, respectively. Absorbance peaks at 625 nm and fluorescence at 675 nm.

         

    Acti-stain phalloidins are the most well characterized phalloidins available. Tabe 1 describes their brightness, photostability, background and affinity constants to F-actin. Compare these performance characteristics to other fluorescent phalloidins and you will see the advantages of using Acti-stain™ for your actin staining requirements.

    Table 1. Biochemical characteristics of fluorescent phalloidins

    Conjugate Cat.#

    Wavelengths (Ex/Em)

    Brightness (AFU*)

    Stability to photobleaching(half life in seconds**)

    Background (% of total AFU at 100nM**)

    Affinity (Kd in nanomolar)

    Fluorescein-phalloidin

    na 485/535 FITC filter set 432 6 22 72 +/-12

    Acti-stain™ 488

    PHDG1 485/535 FITC filter set 832 57 5 55 +/-8

    Acti-stain™ 535

    PHDR1 535/585 TRITC filter set 430 27 12 36 +/-7

    Acti-stain™ 555

    PHDH1 535/585 TRITC filter set 551 46 16 63 +/-8

    Acti-stain™ 670

    PHDN1 640/680 Cy5 filter set 332 18 18 50 +/-12

    * = AFU's measured by quantitative cell imaging. ** = Measured in stained Swiss 3T3 cells in the absence of antifade.

     

    For more information about protocols to fix and stain cells click here.

     


    Manuals and MSDS

    Product Cat.#

    Manual

    MSDS

    Acti-stain™ 488

    PHDG1

    Acti-stain™ 555

    PHDH1

    Acti-stain™ 670

    PHDN1

     

     


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