Product Uses Include
Adenosine triphosphate (ATP). 100 mM solution of ATP at pH 7.0 (to reduce hydrolysis) provided as a lyophilized powder. Required for G-actin stability and F-actin assembly and dynamics.
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Question 1: Can the reconstituted ATP be stored at 4°C for any length of time or does it need to be immediately snap-frozen?
Answer 1: The ATP (Cat. # BSA04) should be reconstituted to 100 mM with 1 ml of cold 100 mM Tris, pH 7.5. Set aside the volume that is needed for the day’s experiment (stable for 4 h on ice) and the remaining ATP should then be aliquoted into experiment-sized amounts, snap frozen in liquid nitrogen, and stored at or below -20°C. These stocks are stable for 6 months at -20°C.
Question 2: How long are solutions with ATP in them stable at 4°C if performing actin polymerization experiments?
Answer 2:Buffers containing ATP (Cat. # BSA04) will be stable for 4 h on ice. Keep on ice until ready to use. After 4 h discard the tube, do not re-freeze to use later. Prepare fresh buffers (or freshly frozen aliquot) for the next experiment.
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