ATP: 100mM stock

ATP: 100mM stock
$0.00

Product Uses Include

  • Addition to general actin buffer
  •  Substrate for ATPases
  • Energy source for kinesin motor activity
  • Energy source for kinases

 

Material
Adenosine triphosphate (ATP). 100 mM solution of ATP at pH 7.0 (to reduce hydrolysis) provided as a lyophilized powder. Required for G-actin stability and F-actin assembly and dynamics.

For product Datasheets and MSDSs please click on the PDF links below.   For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com

AuthorTitleJournalYearArticle Link
Sakuma, Chisako et al.Fibrinopeptide A-induced blood-feeding arrest in the yellow fever mosquito Aedes aegyptiCell Reports2024
Paladini, Serena et al.Measurement and Characterization of the Electrical Properties of Actin FilamentsInternational Journal of Molecular Sciences 2024
Delaunay, Marion et al.AKAP2-anchored extracellular signal-regulated kinase 1 (ERK1) regulates cardiac myofibroblast migrationBiochimica et Biophysica Acta (BBA) - Molecular Cell Research2024
Martin, Annabell et al.A general strategy to develop fluorogenic polymethine dyes for bioimagingNature Chemistry2023
Bashirzadeh, Yashar et al.Encapsulated actomyosin patterns drive cell-like membrane shape changesiScience2022
Pack, Lindsey R. et al.Clinical CDK4/6 inhibitors induce selective and immediate dissociation of p21 from cyclin D-CDK4 to inhibit CDK2Nature Communications2021
Rodríguez-Pérez, Fernando et al.Ubiquitin-dependent remodeling of the actin cytoskeleton drives cell fusionDevelopmental Cell2021
Park, Jin Suk et al.Mechanical regulation of glycolysis via cytoskeleton architectureNature2020
Chung, Mingyu et al.Transient Hysteresis in CDK4/6 Activity Underlies Passage of the Restriction Point in G1Molecular Cell2019
Minutti, Carlos M. et al.A Macrophage-Pericyte Axis Directs Tissue Restoration via Amphiregulin-Induced Transforming Growth Factor Beta ActivationImmunity2019
Martin, Jennifer A. et al.A novel role for the actin-binding protein drebrin in regulating opiate addictionNature Communications2019
Piroli, Gerardo G. et al.Identification of novel protein targets of DMF modification in neurons and astrocytes reveals actions independent of Nrf2 stabilizationMolecular and Cellular Proteomics2019
Patel, Vaibhav B. et al.PI3Kα-regulated gelsolin activity is a critical determinant of cardiac cytoskeletal remodeling and heart diseaseNature Communications2018
Wang, J. et al.Erratum: The small GTPase RhoA, but not Rac1, is essential for conditioned aversive memory formation through regulation of actin rearrangements in rat dorsal hippocampus (Acta Pharmacol Sin (2013) 34 (811-8) Doi: 10.1038/aps.2013.3 )Acta Pharmacologica Sinica2014
Xiao, Xiang et al.c-Yes regulates cell adhesion at the apical ectoplasmic specialization-blood-testis barrier axis via its effects on protein recruitment and distribution2013
Jiwani, Shahanawaz et al.Chlamydia trachomatis Tarp cooperates with the Arp2/3 complex to increase the rate of actin polymerizationBiochemical and Biophysical Research Communications2012
Fan, Jianguo et al.A role for γs-crystallin in the organization of actin and fiber cell maturation in the mouse lensFEBS Journal2012
Butler, Suzanne C. et al.Inhibitory effects of pectenotoxins from marine algae on the polymerization of various actin isoformsToxicology in Vitro2012
Trigili, Chiara et al.Mechanism of Action of the Cytotoxic Macrolides Amphidinolide X and JChemBioChem2011
Tsai, Chih Chien et al.7-Chloro-6-piperidin-1-yl-quinoline-5,8-dione (PT-262), a novel ROCK inhibitor blocks cytoskeleton function and cell migrationBiochemical Pharmacology2011
Takamiya, Rina et al.Overexpression of mutated Cu,Zn-SOD in neuroblastoma cells results in cytoskeletal changeAmerican journal of physiology. Cell physiology2005
Kumar, Narendra et al.Functional dissection and molecular characterization of calcium-sensitive actin-capping and actin-depolymerizing sites in villinThe Journal of biological chemistry2004
Zhai, Liwei et al.Tyrosine phosphorylation of villin regulates the organization of the actin cytoskeletonThe Journal of biological chemistry2001
Fontao, Lionel et al.The interaction of plectin with actin: evidence for cross-linking of actin filaments by dimerization of the actin-binding domain of plectinJournal of cell science2001
Blader, Ira J. et al.GCS1, an Arf guanosine triphosphatase-activating protein in Saccharomyces cerevisiae, is required for normal actin cytoskeletal organization in vivo and stimulates actin polymerization in vitroMolecular Biology of the Cell1999

 

Question 1: Can the reconstituted ATP be stored at 4°C for any length of time or does it need to be immediately snap-frozen?

Answer 1:  The ATP (Cat. # BSA04) should be reconstituted to 100 mM with 1 ml of cold 100 mM Tris, pH 7.5.  Set aside the volume that is needed for the day’s experiment (stable for 4 h on ice) and the remaining ATP should then be aliquoted into experiment-sized amounts, snap frozen in liquid nitrogen, and stored at or below -20°C. These stocks are stable for 6 months at -20°C.

 

Question 2: How long are solutions with ATP in them stable at 4°C if performing actin polymerization experiments?

Answer 2: Buffers containing ATP (Cat. # BSA04) will be stable for 4 h on ice.  Keep on ice until ready to use.  After 4 h discard the tube, do not re-freeze to use later.  Prepare fresh buffers (or freshly frozen aliquot) for the next experiment.

 

 

If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com