Product Uses Include
Adenosine triphosphate (ATP). 100 mM solution of ATP at pH 7.0 (to reduce hydrolysis) provided as a lyophilized powder. Required for G-actin stability and F-actin assembly and dynamics.
|Rodríguez-Pérez, Fernando et al.||Ubiquitin-dependent remodeling of the actin cytoskeleton drives cell fusion||Developmental Cell||2021||ISSN 1878-1551|
|Pack, Lindsey R. et al.||Clinical CDK4/6 inhibitors induce selective and immediate dissociation of p21 from cyclin D-CDK4 to inhibit CDK2||Nature Communications||2021||ISSN 2041-1723|
|Bashirzadeh, Yashar et al.||Encapsulated Actomyosin Patterns Drive Cell-Like Membrane Shape Changes||SSRN Electronic Journal||2021||Article Link|
|Park, Jin Suk et al.||Mechanical regulation of glycolysis via cytoskeleton architecture||Nature||2020||ISSN 1476-4687|
|Chung, Mingyu et al.||Transient Hysteresis in CDK4/6 Activity Underlies Passage of the Restriction Point in G1||Molecular Cell||2019||ISSN 1097-4164|
|Piroli, Gerardo G. et al.||Identification of novel protein targets of dimethyl fumarate modification in neurons and astrocytes reveals actions independent of Nrf2 stabilization||Molecular and Cellular Proteomics||2019||ISSN 1535-9484|
|Martin, Jennifer A. et al.||A novel role for the actin-binding protein drebrin in regulating opiate addiction||Nature Communications||2019||ISSN 2041-1723|
|Minutti, Carlos M. et al.||A Macrophage-Pericyte Axis Directs Tissue Restoration via Amphiregulin-Induced Transforming Growth Factor Beta Activation||Immunity||2019||ISSN 1097-4180|
|Patel, Vaibhav B. et al.||PI3Kα-regulated gelsolin activity is a critical determinant of cardiac cytoskeletal remodeling and heart disease||Nature Communications||2018||ISSN 2041-1723|
|Wang, J. et al.||Erratum: The small GTPase RhoA, but not Rac1, is essential for conditioned aversive memory formation through regulation of actin rearrangements in rat dorsal hippocampus (Acta Pharmacol Sin (2013) 34 (811-8) Doi: 10.1038/aps.2013.3 )||Acta Pharmacologica Sinica||2014||ISSN 1745-7254|
|Xiao, Xiang et al.||c-Yes regulates cell adhesion at the apical ectoplasmic specialization-blood-testis barrier axis via its effects on protein recruitment and distribution||2013||PMID 23169788|
|Fan, Jianguo et al.||A role for γs-crystallin in the organization of actin and fiber cell maturation in the mouse lens||FEBS Journal||2012||ISSN 1742-464X|
|Butler, Suzanne C. et al.||Inhibitory effects of pectenotoxins from marine algae on the polymerization of various actin isoforms||Toxicology in Vitro||2012||ISSN 0887-2333|
|Jiwani, Shahanawaz et al.||Chlamydia trachomatis Tarp cooperates with the Arp2/3 complex to increase the rate of actin polymerization||Biochemical and Biophysical Research Communications||2012||ISSN 0006-291X|
|Tsai, Chih Chien et al.||7-Chloro-6-piperidin-1-yl-quinoline-5,8-dione (PT-262), a novel ROCK inhibitor blocks cytoskeleton function and cell migration||Biochemical Pharmacology||2011||ISSN 0006-2952|
|Trigili, Chiara et al.||Mechanism of Action of the Cytotoxic Macrolides Amphidinolide X and J||ChemBioChem||2011||ISSN 1439-4227|
|Takamiya, Rina et al.||Overexpression of mutated Cu,Zn-SOD in neuroblastoma cells results in cytoskeletal change||American journal of physiology. Cell physiology||2005||ISSN 0363--6143|
|Kumar, Narendra et al.||Functional dissection and molecular characterization of calcium-sensitive actin-capping and actin-depolymerizing sites in villin||The Journal of biological chemistry||2004||ISSN 0021--9258|
|Fontao, Lionel et al.||The interaction of plectin with actin: evidence for cross-linking of actin filaments by dimerization of the actin-binding domain of plectin||Journal of cell science||2001||ISSN 0021--9533|
|Zhai, Liwei et al.||Tyrosine phosphorylation of villin regulates the organization of the actin cytoskeleton||The Journal of biological chemistry||2001||ISSN 0021--9258|
|Blader, Ira J. et al.||GCS1, an Arf guanosine triphosphatase-activating protein in Saccharomyces cerevisiae, is required for normal actin cytoskeletal organization in vivo and stimulates actin polymerization in vitro||Molecular Biology of the Cell||1999||ISSN 1059-1524|
Question 1: Can the reconstituted ATP be stored at 4°C for any length of time or does it need to be immediately snap-frozen?
Answer 1: The ATP (Cat. # BSA04) should be reconstituted to 100 mM with 1 ml of cold 100 mM Tris, pH 7.5. Set aside the volume that is needed for the day’s experiment (stable for 4 h on ice) and the remaining ATP should then be aliquoted into experiment-sized amounts, snap frozen in liquid nitrogen, and stored at or below -20°C. These stocks are stable for 6 months at -20°C.
Question 2: How long are solutions with ATP in them stable at 4°C if performing actin polymerization experiments?
Answer 2: Buffers containing ATP (Cat. # BSA04) will be stable for 4 h on ice. Keep on ice until ready to use. After 4 h discard the tube, do not re-freeze to use later. Prepare fresh buffers (or freshly frozen aliquot) for the next experiment.
If you have any questions concerning this product, please contact our Technical Service department at firstname.lastname@example.org