• Counter-screen system comparing smooth muscle myosin activity to cardiac muscle myosin activity in drug studies
• Add the S1 fragment to actin filaments to determine actin filament polarity in electron microscopy applications

Smooth muscle myosin S1 fragment is prepared by digesting full-length chicken gizzard myosin with -chymotrypsin in the presence of EDTA to liberate the soluble S1 fragment.

• Formulation: supplied as lyophilized powder
• Composition: when reconstituted to 1.4 mg/ml, the buffer composition is: 8 mM PIPES pH 7.0, 8 mM MgCl2, 5% (w/v) sucrose, and 1% (w/v) dextran

Smooth muscle myosin S1 fragment is produced from chicken gizzard myosin that is ≥90% pure

The biological activity of chicken gizzard S1 myosin can be determined from its rate of F-actin-activated ATP hydrolysis. The assay is constructed by first polymerizing actin to form F-actin. Myosin is then added in sub-stoichiometric amounts, and the reaction is initiated with ATP.