As part of the Signal-Seeker™ product line, ubiquitination control beads (CUB02B-beads) have been developed as highly specific negative control beads for Cytoskeleton's ubiquitination affinity bead (UBA01B-Beads) products. These control beads are far superior to beads alone, because it has mutated versions of the UBDs covalently attached to the control bead matrix. This control is particularly important for ubiquitin IPs as the unmodified version of target proteins often bind non-specifically to all available ubiquitin enrichment affinity matrices. A comprehensive Signal-Seeker™ Ubiquitin Detection Kit (BK161), which includes ubiquitin control beads (CUB02B-beads), is available and is recommended for first time users.
Note: The product #s have been updated from UBA01 and CUB02 to UBA01B and CUB02B beads due to a reformulation of the bead preservation buffer. Reformulation results in an enhanced bead performance post-lyophilization. The affinity protein formulation on the beads has not changed.
Application 1: Utilization of IgG control beads
Figure 1: Swiss 3T3 cells were pre-treated with MG-132, then either untreated or treated with CNF1 (CN04) for 3 hours prior to lysis with BlastR buffer. The BK161 kit was utilized to perform the IP on 300 μg of lysate per condition. Input: 3 μg input untreated lysate, Ubiquitin Affinity beads (UBA01) plus untreated lysate, UBA01 plus CN04 treated lysate, ubiquitin control beads (CUB02) plus untreated lysate, and CUB02 plus CN04 treated lysate. Samples were analyzed for Rac1 ubiquitination using an anti-Rac1 antibody.
Each package contains enough ubiquitin control beads for 10 reactions.
For more information contact: email@example.com
Signal-Seeker™ Ubiquitination Detection Kit (Cat. # BK161)
Signal-Seeker™ Ubiquitin Affinity Beads (Cat.# UBA01B-beads)
Signal-Seeker™: BlastR™ Rapid Lysate Prep Kit (Cat. # BLR01)
Signal-Seeker™: PTMtrue™ Ubiquitin Antibody (Cat.# AUB01)
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|Nguyen, Kha The et al.||The MARCHF6 E3 ubiquitin ligase acts as an NADPH sensor for the regulation of ferroptosis||Nature Cell Biology||2022||ISSN 1476--4679|
|Dieter, Sebastian M. et al.||Degradation of CCNK/CDK12 is a druggable vulnerability of colorectal cancer||Cell Reports||2021||ISSN 2211-1247|
|Gu, Chunming et al.||Identification of berberine as a novel drug for the treatment of multiple myeloma via targeting UHRF1||BMC Biology||2020||ISSN 1741-7007|
|Horita, Henrick et al.||Utilizing a comprehensive immunoprecipitation enrichment system to identify an endogenous post-translational modification profile for target proteins||Journal of Visualized Experiments||2018||ISSN 1940-087X|
|Horita, Henrick et al.||A simple toolset to identify endogenous post-translational modifications for a target protein: A snapshot of the EGFR signaling pathway||Bioscience Reports||2017||ISSN 1573-4935|
|Horita, Henrick et al.||Identifying Regulatory Posttranslational Modifications of PD-L1: A Focus on Monoubiquitinaton||Neoplasia (United States)||2017||ISSN 1476-5586|