• Quantitation of total RhoA protein in cell or tissue lysate

Kit contents (96 assays: strip-wells allow single or multiple assays per run)

• Total Rho capture plate
• Anti-RhoA-specific detection antibody
• Secondary antibody: HRP conjugated
• RhoA control protein
• Sample dilution buffer
• Wash buffer
• Antigen-presenting buffer
• HRP colorimetric detection reagents
• Strip-well holder

Equipment & materials required

• Cell or tissue lysate
• Concentrated sulfuric acid (3 ml)
• Multiwell pipette

This is a colorimetric ELISA sandwich assay. Each assay well is coated with an anti-Rho IgY antibody, which has a high affinity for RhoA/B/C. A mouse monoclonal detection antibody is specific for RhoA.

Key characteristics

• Quantitative assay
• Detection sensitivity: Linear between 1.0 – 30 ng RhoA
• Coefficient of variation (cv): 3%
• Detection specificity: RhoA
• Detection method: Colorimetric absorbance at 490 nm
• Input requirements: 3 - 30 µg of cell or tissue lysate per assay
• Timeframe: Approximately 5 hours