Cytoskeleton now offers the first and only commercially available RhoA ELISA.
It is recommended to use the BK150 kit in conjunction with the RhoA G-LISA™ kit (BK124), allowing quantitation of Total RhoA and Active RhoA in the same lysates.
Providing precise solutions to RhoA quantification, enabling researchers to...
Kit Contents - Enough reagents for 96 assays.
|Lee, Sang Joon et al.
|AIM2 forms a complex with pyrin and ZBP1 to drive PANoptosis and host defence
|Porter, Lauren et al.
|SUN1/2 Are Essential for RhoA/ROCK-Regulated Actomyosin Activity in Isolated Vascular Smooth Muscle Cells
|Lachowski, Dariusz et al.
|G Protein-Coupled Estrogen Receptor Regulates Actin Cytoskeleton Dynamics to Impair Cell Polarization
|Frontiers in Cell and Developmental Biology
|Chronopoulos, Antonios et al.
|Syndecan-4 tunes cell mechanics by activating the kindlin-integrin-RhoA pathway
|Ngai, David et al.
|DDR1 (Discoidin Domain Receptor-1)-RhoA (Ras Homolog Family Member A) Axis Senses Matrix Stiffness to Promote Vascular Calcification
|Arteriosclerosis, Thrombosis, and Vascular Biology
|Haq, Naila et al.
|Loss of Bardet-Biedl syndrome proteins causes synaptic aberrations in principal neurons
|Dér, Bálint et al.
|NK2 receptor-mediated detrusor muscle contraction involves Gq/11-dependent activation of voltage-dependent Ca2+ channels and the RhoA-Rho kinase pathway
|American Journal of Physiology - Renal Physiology
|Peng, Jing Hua et al.
|Geniposide and Chlorogenic Acid Combination Ameliorates Non-alcoholic Steatohepatitis Involving the Protection on the Gut Barrier Function in Mouse Induced by High-Fat Diet
|Frontiers in Pharmacology
|Giannini, Marianna et al.
|Nano-topography: Quicksand for cell cycle progression?
|Nanomedicine: Nanotechnology, Biology, and Medicine
|Zhang, Xiaoqing et al.
|Alterations of MEK1/2-ERK1/2, IFNγ and Smad2/3 associated Signalling pathways during cryopreservation of ASCs affect their differentiation towards VSMC-like cells
|Stem Cell Research
|Tod, Jo et al.
|Pro-migratory and TGF-β-activating functions of αvβ6 integrin in pancreatic cancer are differentially regulated via an Eps8-dependent GTPase switch
|Journal of Pathology
|Schillaci, Odessa et al.
|Exosomes from metastatic cancer cells transfer amoeboid phenotype to non-metastatic cells and increase endothelial permeability: Their emerging role in tumor heterogeneity
|Kempf, Anissa et al.
|Control of Cell Shape, Neurite Outgrowth, and Migration by a Nogo-A/HSPG Interaction
|Dyberg, Cecilia et al.
|Rho-associated kinase is a therapeutic target in neuroblastoma
|Proceedings of the National Academy of Sciences of the United States of America
|Yuan, Xue et al.
|Ciliary IFT80 balances canonical versus non-canonical hedgehog signalling for osteoblast differentiation
|Jiang, Lisheng et al.
|Selective activation of CB2 receptor improves efferocytosis in cultured macrophages
|The role of the RhoA/ROCK pathway in gender-dependent differences in gastric smooth muscle contraction
|Journal of Physiological Sciences
|Park, Yong Hwan et al.
|Pyrin inflammasome activation and RhoA signaling in the autoinflammatory diseases FMF and HIDS
|Skrbic, Biljana et al.
|Lack of collagen VIII reduces fibrosis and promotes early mortality and cardiac dilatation in pressure overload in mice
|Escuin, Sarah et al.
|Rho-kinase-dependent actin turnover and actomyosin disassembly are necessary for mouse spinal neural tube closure
|Journal of Cell Science
|Herr, Michael J. et al.
|Tetraspanin CD9 regulates cell contraction and actin arrangement via RhoA in human vascular smooth muscle cells
|Biechler V., Stefanie V. et al.
|The impact of flow-induced forces on the morphogenesis of the outflow tract
|Frontiers in Physiology
|Suen, J. Y. et al.
|Pathway-selective antagonism of proteinase activated receptor 2
|British Journal of Pharmacology
|Tan, Hong et al.
|Fluid flow forces and rhoA regulate fibrous development of the atrioventricular valves
|Valtcheva, Nadejda et al.
|The orphan adhesion G protein-coupled receptor GPR97 regulates migration of lymphatic endothelial cells via the small GTPases RhoA and Cdc42
|Journal of Biological Chemistry
Question 1: Can I use the same lysate samples to measure total RhoA with this kit (Cat. # BK150) and activated RhoA samples in the G-LISA activation assay kit (Cat. # BK124)?
Answer 1: Yes, the same lysate samples can be used in both the total RhoA ELISA (Cat. # BK150) and RhoA G-LISA activation assay kit (Cat. # BK124). When preparing samples to be used with both kits, please use the lysis buffer (Part # GL36) that comes with the RhoA G-LISA assay kit. Generally G‐LISA samples have low protein concentrations, e.g., 0.3 to 0.6 mg/ml. At this concentration the samples are at the lower range of detection for the ELISA. Therefore we recommend using more lysate and less Sample Dilution Buffer when preparing G-LISA samples for the ELISA. To keep the final concentration higher, we recommend using 40 μl of G‐LISA extract plus 80 μl of Sample Dilution Buffer, which is sufficient for duplicate ELISA wells. In this case, also use the same Lysis:SDB ratio for the positive control protein solutions.
Question 2: I see that this ELISA uses a 96 well plate format. Do I have to use all 96 wells at one time or can I save the unused wells for use at a later date?
Answer 2: The 96 well plates are packaged as 12 x 8 well strips. Thus, each strip is removable and can be further broken down to a 1 well format for an assay. In this way, there is tremendous format flexibility since 1-96 wells can be used in an assay. It is imperative to keep the plate in the sealed desiccant bag with desiccant at all times. Move to room temperature 30 min prior to starting the assay. Open the bag and remove the number of strips or wells needed immediately prior to the start of the experiment, place the remaining strips/wells in the desiccated bag, reseal and return to storage at 4°C. Prepare and use the strips/wells as directed.
If you have any questions concerning this product, please contact our Technical Service department at firstname.lastname@example.org.