The BlastR™ filters allows for fast and reliable preparation of genomic DNA (gDNA)-free cell lysate for western blot or immunoprecipitation when used in combination with a denaturing buffer, such as guanidine, urea or high SDS based extraction buffers that usually produce copious viscosity. gDNA contamination is a significant problem with denaturing buffers that can interfere with downstream applications like immunoprecipitation and migration of proteins in SDS-PAGE. Unlike sonication or insulin needle gDNA shearing methods, the BlastR™ filter effectively removes gDNA contamination while having no effect on the integrity of the proteins in the lysate.
Clarification of gDNA from BlastR™ lysate
(A) Viscous sample lysate loaded onto BlastR™ Filter.
(B) Sample passed through Filter system where gDNA is captured. >90% recovery of protein in cell lysate.
|Horita, Henrick et al.||Utilizing a comprehensive immunoprecipitation enrichment system to identify an endogenous post-translational modification profile for target proteins||Journal of Visualized Experiments||2018||ISSN 1940-087X|