+3
Supplied as a lyophilized powder. When resuspended in sterile, deionized water, it provides a 1x working strength buffer consisting of 0.2 mM calcium chloride, 5 mM Tris, pH 8.0.
Before use, the buffer should be supplemented with 0.2 mM adenosine 5’ triphosphate disodium salt (ATP) BSA04 to maintain active actin monomer.
For actin polymerization see Cat.# BSA02
All reagents used in this buffer are ≥ American Chemical Society (ACS) grade and ≥99% pure
The biological activity of BSA01 is confirmed by its ability to maintain rabbit muscle actin in a polymerization-competent form.
Cat. #BSA01
