General actin buffer: 1x stock

$0.00

SKU

BSA01

Datasheet

MSDS

Amount

1x10 ml (Cat. # BSA01-001) + $77.00

1x100 ml (Cat. # BSA01-010) + $171.00

Qty:

Details

Product Uses Include

• Resuspension of actin protein
• Dilution and storage of G-actin protein

Material
This buffer contains 5 mM Tris-HCl pH 8.0 and 0.2 mM CaCl₂. Used as a general G-actin (monomer) buffer with the addition of 0.2 mM ATP (see Cat. # BSA04) and 0.5 mM DTT (A-buffer). The buffer can be changed to a general F-actin (filament supporting) buffer by the addition of 1/10th volume of actin polymerization buffer (see Cat. # BSA02).

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About

For product Datasheets and MSDSs please click on the PDF links below. For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com

Citations

Author	Title	Journal	Year	Article Link
Paladini, Serena et al.	Measurement and Characterization of the Electrical Properties of Actin Filaments	International Journal of Molecular Sciences	2024	ISSN 1422--0067
Delaunay, Marion et al.	AKAP2-anchored extracellular signal-regulated kinase 1 (ERK1) regulates cardiac myofibroblast migration	Biochimica et Biophysica Acta (BBA) - Molecular Cell Research	2024	ISSN 0167--4889
Martin, Annabell et al.	A general strategy to develop fluorogenic polymethine dyes for bioimaging	Nature Chemistry	2023	ISSN 1755--4349
Park, Jin Suk et al.	Mechanical regulation of glycolysis via cytoskeleton architecture	Nature	2020	ISSN 1476-4687
Limatola, Nunzia et al.	Nicotine Induces Polyspermy in Sea Urchin Eggs through a Non-Cholinergic Pathway Modulating Actin Dynamics	Cells	2019	ISSN 2073-4409
Laitila, Jenni et al.	A nebulin super-repeat panel reveals stronger actin binding toward the ends of the super-repeat region	Muscle and Nerve	2019	ISSN 1097-4598
Da’as, Sahar I. et al.	Hypertrophic cardiomyopathy-linked variants of cardiac myosin-binding protein C3 display altered molecular properties and actin interaction	Biochemical Journal	2018	ISSN 1470-8728
Patel, Vaibhav B. et al.	PI3Kα-regulated gelsolin activity is a critical determinant of cardiac cytoskeletal remodeling and heart disease	Nature Communications	2018	ISSN 2041-1723
Reeg, Sandra et al.	The molecular chaperone Hsp70 promotes the proteolytic removal of oxidatively damaged proteins by the proteasome	Free Radical Biology and Medicine	2016	ISSN 1873-4596
Goryunov, D et al.	Microtubule-actin cross-linking factor 1: domains, interaction partners, and tissue-specific functions	Methods in Enzymology	2016	Article Link
Wang, Y et al.	Fluorescence imaging with one-nanometer accuracy (FIONA)	JoVE (Journal of …	2014	Article Link
Xiao, Xiang et al.	c-Yes regulates cell adhesion at the apical ectoplasmic specialization-blood-testis barrier axis via its effects on protein recruitment and distribution		2013	PMID 23169788
Metcalf, Daniel J. et al.	Test samples for optimizing storm super-resolution microscopy	Journal of Visualized Experiments	2013	ISSN 1940-087X
Jiwani, Shahanawaz et al.	Chlamydia trachomatis Tarp cooperates with the Arp2/3 complex to increase the rate of actin polymerization	Biochemical and Biophysical Research Communications	2012	ISSN 0006-291X
Fan, Jianguo et al.	A role for γs-crystallin in the organization of actin and fiber cell maturation in the mouse lens	FEBS Journal	2012	ISSN 1742-464X
Butler, Suzanne C. et al.	Inhibitory effects of pectenotoxins from marine algae on the polymerization of various actin isoforms	Toxicology in Vitro	2012	ISSN 0887-2333
Trigili, Chiara et al.	Mechanism of Action of the Cytotoxic Macrolides Amphidinolide X and J	ChemBioChem	2011	ISSN 1439-4227
Tsai, Chih Chien et al.	7-Chloro-6-piperidin-1-yl-quinoline-5,8-dione (PT-262), a novel ROCK inhibitor blocks cytoskeleton function and cell migration	Biochemical Pharmacology	2011	ISSN 0006-2952
Cheng, Chao Min et al.	Creating cellular and molecular patterns via gravitational force with liquid droplets	Applied Physics Letters	2008	ISSN 0003-6951
Takamiya, Rina et al.	Overexpression of mutated Cu,Zn-SOD in neuroblastoma cells results in cytoskeletal change	American journal of physiology. Cell physiology	2005	ISSN 0363--6143
Kumar, Narendra et al.	Functional dissection and molecular characterization of calcium-sensitive actin-capping and actin-depolymerizing sites in villin	The Journal of biological chemistry	2004	ISSN 0021--9258
Goryunov, Dmitry et al.	Studying cytolinker proteins	Methods in Cell Biology	2004	ISSN 0091-679X
Zhai, Liwei et al.	Tyrosine phosphorylation of villin regulates the organization of the actin cytoskeleton	The Journal of biological chemistry	2001	ISSN 0021--9258
Fontao, Lionel et al.	The interaction of plectin with actin: evidence for cross-linking of actin filaments by dimerization of the actin-binding domain of plectin	Journal of cell science	2001	ISSN 0021--9533
Blader, Ira J. et al.	GCS1, an Arf guanosine triphosphatase-activating protein in Saccharomyces cerevisiae, is required for normal actin cytoskeletal organization in vivo and stimulates actin polymerization in vitro	Molecular Biology of the Cell	1999	ISSN 1059-1524

Faqs

Question 1: Can the general actin buffer be stored with ATP added or should it be added fresh?

Answer 1: On the day of the experiment, the general actin buffer (Cat. # BSA01) should be supplemented with 0.2 mM ATP (Cat. # BSA04) to create an optimal actin monomer buffer. We do not recommend storing the actin buffer with ATP in it. Prepare the actin buffer + ATP in a volume that is needed for the experiment. Calcium ions and ATP are required for actin conformation. In addition, ATP is hydrolysed during actin polymerization and is required for the polymerization process.

Question 2: What is the composition of Cytoskeleton’s general actin buffer?

Answer 2: Upon resuspension in 100 ml of sterile de-ionized water, the buffer (Cat. # BSA01) will be at 1X strength buffer and contain: 0.2 mM calcium chloride and 5 mM Tris-HCl, pH 8.0.

If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com