• In vitro assays that require highly pure actin (>99%)
• In vitro characterization of actin binding proteins (ABPs). NOTE: Actin is a highly conserved protein and AKL99 serves as a cost-effective substrate for most ABPs
• In vitro study of actin dynamics

Alpha-skeletal actin protein has been purified from rabbit skeletal muscle and has an approximate molecular weight of 43 kDa. AKL99 is supplied as a white lyophilized powder.

Protein purity is assessed using scanning densitometry of Coomassie-stained SDS-PAGE gels. AKL99 is >99% pure alpha-skeletal muscle actin.

The biological activity of muscle actin is assessed by its ability to efficiently polymerize into filaments in vitro, which can be separated from unpolymerized components using a spin-down assay. Rigorous quality control ensures that >90% of the muscle actin polymerizes under these conditions.