• In vitro motility assays with myosin proteins
• In vitro modeling of the cytoskeleton in the cell cortex
• Study in vivo actin dynamics by labeling of free barbed ends of actin filaments
• Study actin-binding proteins (ABPs)

Rabbit skeletal muscle actin AKL99 is randomly labeled on surface lysines with an activated ester of HiLyte™488 fluorescent dye:

• Ex: 499 nm, Em: 523 nm – green spectrum
• Labeling stoichiometry: ~0.4-0.6 dye per actin monomer.
• Supplied as a lyophilized powder for fluorescence microscopy

Protein purity is assessed by scanning densitometry of Coomassie Blue-stained protein on a12% polyacrylamide gel. Purity was determined to be >99% pure.

The biological activity of HiLyte™488 actin can be determined by its ability to polymerize into filaments in vitro efficiently and separate from unpolymerized components in a spin-down assay. Stringent quality control ensures that ≥90% of the labeled actin can polymerize.