• Substrate for microtubule (MT) activated kinesin ATPase assay
• Screen for KIF18A inhibitors
• Counter-screen for kinesin drug candidates

KIF18A is a kinesin motor protein critical for chromosome alignment and spindle microtubule regulation during mitosis, with dysfunction linked to chromosomal instability in cancers. Emerging studies suggest KIF18A inhibition may enhance the effectiveness of cancer therapy, making it a potential target for kinesin inhibitors in oncology research.

The wild-type human motor domain of KIF18 is produced in a bacterial expression system.

• Protein tag: N-terminal 6x His tag
• Molecular weight: 44 kDa
• Motor amino acids: 1-374
• Uniprot ID: Q8N177
• Formulation: supplied as lyophilized powder
• Composition: when reconstituted to 5 mg/ml, the buffer composition is: 100 mM Tris pH 8.0, 100 mM NaCl, 2 mM MgCl2, 15% (w/v) sucrose, and 1% (w/v) dextran

Protein purity is assessed by scanning densitometry of Coomassie Blue-stained protein on a4-20% polyacrylamide gel. Purity is determined to be >80% pure.

Biological activity of CS-KF18A is measured using a microtubule-activated ATPase assay. Under the conditions stated (see datasheet), KIF18A exhibits a microtubule-stimulated ATPase activity with a Vmax of ≥ 1500 nmol of ATP produced per minute per mg of KIF18A.