Compound Screening

Cytoskeleton, Inc. is a reliable source of compound screening services for developing tubulin inhibitors, microtubule inhibitors, myosin inhibitors and the sarcomere inhibitors, kinesin inhibitors and small G-protein inhibitors. Over the past 20 years numerous companies have contracted with us to provide  early discovery data, regular data for SAR based compound optimization and pre-clinical drug development analysis and validation. In all cases the client was provided with regular updates as their project progressed, and timely completion and full project details were recorded in a comprehensive report. Our experts can provide in depth knowledge and advice in the cytoskeleton and signal transduction areas, which is suitable for SAR based research and primary and secondary screening. Target proteins have rapidly available assays include the GEFs (Dbs, LARG, Ras-GRF, SOS1, Tiam1, Vav1, Vav2), kinesins (Eg5, KIF5, KIF1C, KIFC1, KIF, KIF3C, KIFC3, MKLP1, MKLP2, MCAK, CenPE, Chromokinesin), myosins (myosin S1, heavy merosmyosin, calcium sensitive soluble sarcomeric atpase), small g-proteins (N-Ras, H-Ras, K-Ras, R-Ras, RhoA, RhoB, RhoC, Rac1, Rac2, Rac3, Cdc42, Arf1, Arf6), and the tubulin targets like neuronal tubulin, microtubules and species specific FtsZ proteins, as well as specialized tubulins such as cancer cell line or tumor tubulin, plant tubulin and fungal tubulin.

 

We have introduced a modular format to the assays which allow easier recognition of the suitability of each format for your project's purposes. The readily available modules are summarized below. More details are available by click on the About tab above.

 

Click on a link below to see the full range of assays:

 

Actin Polymerization Assays

FtsZ GTPase and Polymerization Assays

GTP Exchange Factor (GEF) Exchange Assays

GTPase Activating Protein Assays

Kinesin ATPase Assays 

Myosin ATPase Assays

Tubulin Polymerization Assays

 

If you have an immediate question please select from the buttons above or send an e-mail describing your requirements to tservice@cytoskeleton.com


Screen name

Amount

Module Code

Tubulin polymerization (colorimetric, 340nm)

32 assays (1 IC50 + 1 Control IC50)

CDS001

Tubulin polymerization (colorimetric, 340nm)

32 assays (14 duplicates + 2 Controls + 2 blanks)

CDS002

Tubulin polymerization (colorimetric, 340nm)

96 assays (5 IC50 + 1 Control IC50)

CDS003

Tubulin polymerization (colorimetric, 340nm)

96 assays (40 duplicates + 4 Controls + 4 blanks)

CDS004

Tubulin polymerization (colorimetric, 340nm)

192 assays (10 IC50 + 2 Control IC50)

CDS005

Tubulin polymerization (colorimetric, 340nm)

192 assays (80 duplicates + 8 Controls + 8 blanks)

CDS006

Tubulin polymerization (colorimetric, 340nm)

384 assays (20 IC50 + 4 Control IC50)

CDS007

Tubulin polymerization (colorimetric, 340nm)

384 assays (160 duplicates + 16 Controls + 16 blanks)

CDS008

   

Tubulin polymerization (fluorescence, 360nm/410nm)

96 assays (5 IC50 + 1 Control IC50)

CDS009

Tubulin polymerization (fluorescence,. 360nm/410nm)

96 assays (40 duplicates + 4 Controls + 4 blanks)

CDS010

Tubulin polymerization (fluorescence, 360nm/410nm)

192 assays (10 IC50 + 2 Control IC50

CDS011

Tubulin polymerization (fluorescence, 360nm/410nm)

192 assays (80 duplicates + 8 Controls + 8 blanks)

CDS012

Tubulin polymerization (fluorescence, 360nm/410nm)

384 assays (20 IC50 + 4 Control IC50

CDS013

Tubulin polymerization (fluorescence,. 360nm/410nm)

384 assays (160 duplicates + 16 Controls + 16 blanks)

CDS014

   

Tubulin polymerization (fluorescence, 360nm/410nm)

60 assays (5 IC50 + 1 Control IC50)

CDS015

Tubulin polymerization (fluorescence, 360nm/410nm)

60 assays (28 duplicates + 4 Controls)

CDS016

Tubulin polymerization (fluorescence, 360nm/410nm)

60 assays (5 IC50 + 1 Control IC50)

CDS017

Tubulin polymerization (fluorescence, 360nm/410nm)

60 assays (28 duplicates + 4 Controls)

CDS018

Tubulin polymerization (fluorescence, 360nm/410nm)

60 assays (5 IC50 + 1 Control IC50)

CDS019

Tubulin polymerization (fluorescence, 360nm/410nm)

60 assays (28 duplicates + 4 Controls)

CDS020

   

Kinesin Motor assays (650nm)

96-endpoint (5 IC50 + 1 Control IC50)

CDS050

Kinesin Motor assays (650nm)

96-endpoint (40 duplicates + 4 Controls + 4 blanks)

CDS051

Kinesin Motor assays (360nm)

96-kinetic (5 IC50 + 1 Control IC50)

CDS052

Kinesin Motor assays (360nm)

96-kinetic (40 duplicates + 4 Controls + 4 blanks)

CDS053

 

 

 

Myosin Motor assays (650nm)

96-endpoint (5 IC50 + 1 Control IC50)

CDS054

Myosin Motor assays (650nm)

96-endpoint (40 duplicates + 4 Controls + 4 blanks)

CDS055

Myosin Motor assays (360nm)

96-kinetic (5 IC50 + 1 Control IC50)

CDS056

Myosin Motor assays (360nm)

96-kinetic (40 duplicates + 4 Controls + 4 blanks)

CDS057

 

 

 

 Sarcomere assay (650nm), contains myosin S1 fragment (motor domain) with tropomyosin / troponin(T/C/I) complex and actin filaments. 

 96-endpoint (5 IC50 + 1 Control IC50)

CDS058 

 Sarcomere assay (650nm), contains myosin S1 fragment (motor domain) with tropomyosin / troponin(T/C/I) complex and actin filaments. 

96-endpoint (40 duplicates + 4 Controls + 4 blanks)

CDS059

 Sarcomere assay (360nm), contains myosin S1 fragment (motor domain) with tropomyosin / troponin(T/C/I) complex and actin filaments. 

96-kinetic (5 IC50 + 1 Control IC50)

CDS060 

 Sarcomere assay (360nm), contains myosin S1 fragment (motor domain) with tropomyosin / troponin(T/C/I) complex and actin filaments. 

96-kinetic (40 duplicates + 4 Controls + 4 blanks)

CDS061 

   
Dynein Motor assay 96 endpoint assays, (5 IC50 + 1 Control IC50)CDS062
Dynein Motor assay 96 endpoint assays, 40 duplicates + 4 Controls + 4 blanksCDS063
   

Actin (skeletal muscle) polymerization

96 assays (5 IC50s + 1 control)

CDS080

Actin (skeletal muscle) polymerization

96 assays (40 duplicates + 4 Controls + 4 blanks)

CDS081

Actin (non-muscle) polymerization

96 assays (5 IC50s + 1 control)

CDS082

Actin (non-muscle) polymerization

96 assays (40 duplicates + 4 Controls + 4 blanks)

CDS083

   
FtsZ GTPase assay (650nm, endpoint)

96 assays (5 IC50s + 1 control)

CDS250
FtsZ GTPase assay (650nm, endpoint)

96 assays (40 duplicates + 4 Controls + 4 blanks)

CDS251
FtsZ GTPase assay (360nm, kinetic)

96 assays (5 IC50s + 1 control)

CDS252
FtsZ GTPase assay (360nm, kinetic)

96 assays (40 duplicates + 4 Controls + 4 blanks)

CDS253
   

F-Actin Binding Spin-down Assays (skeletal muscle actin)

24 assays (10 duplicates, four controls.

CDS090

F-Actin Binding Spin-down Assays (non muscle actin)

24 assays (10 duplicates, four controls.

CDS091

   

GEF Assays

96 assays (40 duplicates + 4 Controls + 4 blanks)

CDS100

GAP Assays

96 assays (40 duplicates + 4 Controls + 4 blanks)

CDS105

 

 

 

Tubulin ELISA (alpha) (total tubulin)

96 assays (40 duplicates + 4 Controls + 4 blanks)

CDS200

Tubulin ELISA (beta I, II, III, IV, V or VI)

96 assays (40 duplicates + 4 Controls + 4 blanks)

CDS201

Tubulin ELISA (acetylated alpha)

96 assays (40 duplicates + 4 Controls + 4 blanks)

CDS202

Tubulin ELISA (glutamylated)

96 assays (40 duplicates + 4 Controls + 4 blanks)

CDS203

Tubulin ELISA (glycinated)

96 assays (40 duplicates + 4 Controls + 4 blanks)

CDS204

Tubulin ELISA (tyrosinated)

96 assays (40 duplicates + 4 Controls + 4 blanks)

CDS205

 

 

 

GLISA with extracts provided by Customer

48 assays (20 duplicates + 4 Controls + 4 blanks)

CDS220

 

  

GLISA with extracts made at Cytoskeleton

48 assays (20 duplicates + 4 Controls + 4 blanks)

CDS221

   

RhoA ELISA (total RhoA)

96 assays (40 duplicates + 4 Controls + 4 blanks)

CDS222

 

 

 For more information click the Documents tab above.

Screening Modules available at short notice 

Screen name

 Target protein

Amount

 Deliverables

 Timeline (weeks)

Module Code

Tubulin polymerization (col. 340nm)

 Pure porcine (T240) or bovine (TL238) brain tubulin.

32 assays (1 IC50 + 1 Control IC50)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS001

Tubulin polymerization (col. 340nm)

  Pure porcine (T240) or bovine (TL238) brain tubulin.

32 assays (14 duplicates + 2 Controls + 2 blanks)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS002

Tubulin polymerization (col. 340nm)

  Pure porcine (T240) or bovine (TL238) brain tubulin.

96 assays (5 IC50 + 1 Control IC50)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS003

Tubulin polymerization (col. 340nm)

  Pure porcine (T240) or bovine (TL238) brain tubulin.

96 assays (40 duplicates + 4 Controls + 4 blanks)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS004

Tubulin polymerization (col. 340nm)

  Pure porcine (T240) or bovine (TL238) brain tubulin.

192 assays (10 IC50 + 2 Control IC50)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 4

CDS005

Tubulin polymerization (col. 340nm)

  Pure porcine (T240) or bovine (TL238) brain tubulin.

192 assays (80 duplicates + 8 Controls + 8 blanks)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 4

CDS006

Tubulin polymerization (col. 340nm)

  Pure porcine (T240) or bovine (TL238) brain tubulin.

384 assays (20 IC50 + 4 Control IC50)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS007

Tubulin polymerization (col. 340nm)

  Pure porcine (T240) or bovine (TL238) brain tubulin.

384 assays (160 duplicates + 16 Controls + 16 blanks)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 4

CDS008

      

Tubulin polymerization (flr. 360nm/410nm)

  Pure porcine (T240) or bovine (TL238) brain tubulin.

 

96 assays (5 IC50 + 1 Control IC50)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS009

Tubulin polymerization (flr. 360nm/410nm)

  Pure porcine (T240) or bovine (TL238) brain tubulin.

 

96 assays (40 duplicates + 4 Controls + 4 blanks)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS010

Tubulin polymerization (flr. 360nm/410nm)

  Pure porcine (T240) or bovine (TL238) brain tubulin.

 

192 assays (10 IC50 + 2 Control IC50

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 4

CDS011

Tubulin polymerization (flr. 360nm/410nm)

  Pure porcine (T240) or bovine (TL238) brain tubulin.

 

192 assays (80 duplicates + 8 Controls + 8 blanks)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 4

CDS012

Tubulin polymerization (flr. 360nm/410nm)

  Pure porcine (T240) or bovine (TL238) brain tubulin.

 

384 assays (20 IC50 + 4 Control IC50

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS013

Tubulin polymerization (flr. 360nm/410nm)

  Pure porcine (T240) or bovine (TL238) brain tubulin.

 

384 assays (160 duplicates + 16 Controls + 16 blanks)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 4

CDS014

      

Tubulin polymerization (flr. 360nm/410nm)

 Pure tubulin isolated from Caki-1 tumor, HeLa cells, MCF-7 cells, insect cells, Soybean germlings or fungal cells. 

60 assays (5 IC50 + 1 Control IC50)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 4

CDS015

Tubulin polymerization (flr. 360nm/410nm)

 Pure tubulin isolated from Caki-1 tumor, HeLa cells, MCF-7 cells, insect cells, Soybean germlings or fungal cells. 

60 assays (28 duplicates + 4 Controls)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 4

CDS016

Tubulin polymerization (flr. 360nm/410nm)

 Pure tubulin isolated from Caki-1 tumor, HeLa cells, MCF-7 cells, insect cells, Soybean germlings or fungal cells. 

60 assays (5 IC50 + 1 Control IC50)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 4

CDS017

Tubulin polymerization (flr. 360nm/410nm)

 Pure tubulin isolated from Caki-1 tumor, HeLa cells, MCF-7 cells, insect cells, Soybean germlings or fungal cells. 

60 assays (28 duplicates + 4 Controls)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 4

CDS018

Tubulin polymerization (flr. 360nm/410nm)

 Pure tubulin isolated from Caki-1 tumor, HeLa cells, MCF-7 cells, insect cells, Soybean germlings or fungal cells. 

60 assays (5 IC50 + 1 Control IC50)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 4

CDS019

Tubulin polymerization (flr. 360nm/410nm)

 Pure tubulin isolated from Caki-1 tumor, HeLa cells, MCF-7 cells, insect cells, Soybean germlings or fungal cells. 

 

60 assays (28 duplicates + 4 Controls)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 4

CDS020

      

Kinesin Motor assays (650nm)

 Any of the available kinesin motors in the CytoWerks Product Line,  or MKLP2, KIF7, KIF1C or KIF1B motor domains in a reation with porcine brain microtubules.

96-endpoint (5 IC50 + 1 Control IC50)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS050

Kinesin Motor assays (650nm)

 Any of the available kinesin motors in the CytoWerks Product Line,  or MKLP2, KIF7, KIF1C or KIF1B  motor domains in a reation with porcine brain microtubules.

96-endpoint (40 duplicates + 4 Controls + 4 blanks)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS051

Kinesin Motor assays (360nm)

 Any of the available kinesin motors in the CytoWerks Product Line,  or MKLP2, KIF7, KIF1C or KIF1B motor domains in a reation with porcine brain microtubules.

96-kinetic (5 IC50 + 1 Control IC50)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS052

Kinesin Motor assays (360nm)

 Any of the available kinesin motors in the CytoWerks Product Line,  or MKLP2, KIF7, KIF1C, or KIF1B motor domains in a reation with porcine brain microtubules.

96-kinetic (40 duplicates + 4 Controls + 4 blanks)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS053

 

 

 

 

 

 

Myosin Motor assays (650nm)

 Myosin II from rabbit skeletal muscle or bovine heart, S1 fragment (motor domain) from bovine heart, smooth muscle (chicken gizzard), skeletal muscle (rabbit) or non-muscle (bovine spleen), in a reaction with actin filaments.

96-endpoint (5 IC50 + 1 Control IC50)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS054

Myosin Motor assays (650nm)

 Myosin II from rabbit skeletal muscle or bovine heart, S1 fragment (motor domain) from bovine heart, smooth muscle (chicken gizzard), skeletal muscle (rabbit) or non-muscle (bovine spleen), in a reaction with actin filaments.

96-endpoint (40 duplicates + 4 Controls + 4 blanks)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS055

Myosin Motor assays (360nm)

 Myosin II from rabbit skeletal muscle or bovine heart, S1 fragment (motor domain) from bovine heart, smooth muscle (chicken gizzard), skeletal muscle (rabbit) or non-muscle (bovine spleen), in a reaction with actin filaments.

96-kinetic (5 IC50 + 1 Control IC50)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS056

Myosin Motor assays (360nm)

 Myosin II from rabbit skeletal muscle or bovine heart, S1 fragment (motor domain) from bovine heart, smooth muscle (chicken gizzard), skeletal muscle (rabbit) or non-muscle (bovine spleen), in a reaction with actin filaments.

96-kinetic (40 duplicates + 4 Controls + 4 blanks)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS057

 

 

 

 

 

 

 Sarcomere assay (360nm)

 Myosin S1 fragment (motor domain) from bovine heart, smooth muscle (chicken gizzard) or skeletal muscle (rabbit) in a reaction with tropomyosin / troponin(T/C/I) complex and actin filaments. 

 96-endpoint (5 IC50 + 1 Control IC50)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS058

 Sarcomere assay (360nm)

 Myosin S1 fragment (motor domain) from bovine heart, smooth muscle (chicken gizzard) or skeletal muscle (rabbit) in a reaction with tropomyosin / troponin(T/C/I) complex and actin filaments. 

 96-endpoint (40 duplicates + 4 Controls + 4 blanks)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS059

 Sarcomere assay (360nm)

 Myosin S1 fragment (motor domain) from bovine heart, smooth muscle (chicken gizzard) or skeletal muscle (rabbit) in a reaction with tropomyosin / troponin(T/C/I) complex and actin filaments. 

 96-kinetic (5 IC50 + 1 Control IC50)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS060

 Sarcomere assay (360nm)

 Myosin S1 fragment (motor domain) from bovine heart, smooth muscle (chicken gizzard) or skeletal muscle (rabbit) in a reaction with tropomyosin / troponin(T/C/I) complex and actin filaments. 

 96-kinetic (40 duplicates + 4 Controls + 4 blanks)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS061

      
Dynein Motor assay (650nm)Cytoplasmic dynein isolated from porcine brain in a reaction with ATP and microtubules. Ciliobrevin-D as control.96-endpoint (5 IC50 + 1 Control IC50)

PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.
3CDS062
Dynein Motor assay (650nm)Cytoplasmic dynein isolated from porcine brain in a reaction with ATP and microtubules. Ciliobrevin-D as control.96-endpoint (40 duplicates + 4 Controls + 4 blanks)
PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.
3CDS063
      

Actin (skeletal muscle) polymerization

 Pyrene muscle actin (AP05)

96 assays (5 IC50s + 1 control)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS080

Actin (skeletal muscle) polymerization

 Pyrene muscle actin (AP05)

96 assays (40 duplicates + 4 Controls + 4 blanks)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS081

Actin (non-muscle) polymerization

 90% non-muscle actin (APHL99, beta:gamma isoforms in 4:1 ratio), plus 10% pyrene muscle actin (AP05) as a marker.

96 assays (5 IC50s + 1 control)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS082

Actin (non-muscle) polymerization

 90% non-muscle actin (APHL99, beta:gamma isoforms in 4:1 ratio), plus 10% pyrene muscle actin (AP05) as a marker.

96 assays (40 duplicates + 4 Controls + 4 blanks)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS083

      

F-Actin Binding Spin-down Assays (skeletal muscle actin)

 Rabbit skeletal muscle actin.

24 assays (10 duplicates, four controls.

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS090

F-Actin Binding Spin-down Assays (non muscle actin)

 Bovine spleen non-muscle actin (ABS99) or human platelet non-muscle actin (APHL99).

24 assays (10 duplicates, four controls.

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS091

      

GEF Assays

 hDBS with Rho or Cdc42, or ARNO with Arf1 a-del13 or Arf6 a-del17.

96 assays (40 duplicates + 4 Controls + 4 blanks)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS100

GAP Assays

 p50 Rho GAP with Rho, Rac or Cdc42, or other provided GAP / small G-protein combination.

96 assays (40 duplicates + 4 Controls + 4 blanks)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS105

 

 

   

 

Tubulin ELISA (alpha) (total tubulin)

 Anti-alpha-tubulin mouse monoclonal antibody.

96 assays (40 duplicates + 4 Controls + 4 blanks)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS200

Tubulin ELISA (beta I, II, III, IV, V or VI)

 Anti-beta-tubulin isoform mouse monoclonal antibody.

96 assays (40 duplicates + 4 Controls + 4 blanks)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS201

Tubulin ELISA (acetylated alpha)

 Anti-acetylated tubulin mouse monoclonal antibody.

96 assays (40 duplicates + 4 Controls + 4 blanks)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS202

Tubulin ELISA (glutamylated)

 Anti-glutamylated tubulin mouse monoclonal antibody.

96 assays (40 duplicates + 4 Controls + 4 blanks)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS203

Tubulin ELISA (glycinated)

 Anti-glycinated tubulin mouse monoclonal antibody.

96 assays (40 duplicates + 4 Controls + 4 blanks)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS204

Tubulin ELISA (tyrosinated)

 Anti-tyrosinated tubulin mouse monoclonal antibody.

96 assays (40 duplicates + 4 Controls + 4 blanks)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS205

 

 

 

 

 

 

GLISA with extracts provided by Customer

 Rho, Rac, Cdc42, Ras, Ral, Arf1 or Arf6 GLISA kit.

48 assays (20 duplicates + 4 Controls + 4 blanks)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS220

 

 

    

GLISA with extracts made at Cytoskeleton

 Rho, Rac, Cdc42, Ras, Ral, Arf1 or Arf6 GLISA Kit.

48 assays (20 duplicates + 4 Controls + 4 blanks)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 6

CDS221

      

RhoA ELISA (total RhoA)

 Rho ELISA Kit (BK150)

96 assays (40 duplicates + 4 Controls + 4 blanks)

 PDF report with Executive Summary, Introduction, Methods, Results and Data Analysis.

 3

CDS222

 

Cytoskeleton provides custom services on a confidential basis, as such there are limited published citations of our work. Representative examples of publications coming from our services are 1) Tubulin polymerization assays (ref.1)  and plant tubulin purification (ref.2).   We can also provide professional references of services performed upon request.

 

References:

 

1)  In Vitroand In VivoAnticancer Activities of Synthetic Macrocyclic Ketone Analogues of Halichondrin B1. CANCER RESEARCH 61, 1013–1021, February 1, 2001. Murray J. Towle, Kathleen A. Salvato, Jacqueline Budrow, Bruce F. Wels, Galina Kuznetsov, Kimberley K. Aalfs, Susan Welsh, Wanjun Zheng, Boris M. Seletsky, Monica H. Palme, Gregory J. Habgood, Lori A. Singer, Lucian V. DiPietro, Yuan Wang, Jack J. Chen, David A. Quincy, Ashley Davis, Kentaro Yoshimatsu, Yoshito Kishi, Melvin J. Yu, and Bruce A. Littlefield2 (2001).

 

2) The herbicide flamprop-M-methyl has a new antimicrotubule mechanism of action.Stefan Tresch, Ricarda Niggeweg and Klaus Grossmann. Pest Manag Sci64:1195–1203 (2008).

 

Cytoskeleton, Inc. has been a reliable source of protein, assays and drug screening services for nearly two decades. Past clients include:

 

Merck         Eli Lilly         Eisai       Amgen         Abbott Labs        Zentaris       GlaxoSmithKline

 

Genentech       Johnson & Johnson       Bristol-Myers Squibb        Pfizer        Wyeth

If you have an immediate question please send an e-mail describing your requirements to tservice@cytoskeleton.com