Protein Purification

Cytoskeleton, Inc. has been a reliable source of protein purification services in the areas of recombinant and native source proteins. We emphasize high purity and biological activity which enables the proteins to be used in many applications including diagnostic kits, pre-clinical drug development programs, early compound screening in primary HTS projects, and in ELISA formats. We also have extensive experience in gene design and expression with an eye to produce the correct orientation and solubility for particular applications. Many purifications use the unique skills and expertise in cytoskeletal and signal transductions proteins. Using this combined knowledge we have produced many functional assays for kinesins (e.g. Eg5, CenPE, MKLP2), myosins (e.g. cardiac, smooth, skeletal and non-muscle isoforms, sarcomere assays), small G-proteins (e.g. Rho, Arf, Ral families), tubulins (e.g. tumor, plant and fungal origins), tubulin binding proteins (e.g. tau and MAPs) and actin binding proteins (e.g. cofilin, profiling, gelsolin), many of them are multi-protein assays that might have protein complexes of 3 or more subunits e.g. a soluble sarcomere format and the Arp2/3 complex based assay.

 

A particular speciality of Cytoskeleton's design formts is the ability to rapidly create lyophilized formulations in appropriate daily use or weekly use amounts. This enables HTS use and reproducible daily performance. Other experience in antibody and  ELISA technology and protein synthesis complements the cytoskeletal and signal transduction focus. 

 

We support all of our services with a dedicated technical services department and years of laboratory experience in the fields of protein biochemistry, cell biology, cancer biology, cardio-vascular research and neuroscience.

 

For more details in protein purification services and modules click on the About tab above.

 

For rapidly available proteins i.e. less than two weeks delivery, see the table below.

 

If you have an immediate question please select from the buttons above or send an e-mail describing your requirements to tservice@cytoskeleton.com

In this section we describe more about the process of producing a reliable source of protein, the deliverables and the timelines.

The Process of producing your protein of interest:

Initial inquiry > Formulate scope and timeline > Generate quotation > Initiate project with a Purchase Order > Receive weekly updates > Receive product and datasheet.

 

The intial inquiry can be as simple as an e-mail to our Technical Service Department, or to fill out the attached form (click for Form). We will get back to you with some clarification questions and a proposal for scope (gene construct, purification tag, protein activity and purity), batch size and timeline. If those are acceptable conditions then we will generate a formal quote which is useful for passing through your Purchasing process and generating a Purchase Order (PO). When we receive the PO the work can start, we will give you a more detailed plan and keep you informed with weekly updates. At the end of the project we will ship the proteins in a desiccated chamber and provide a datasheet which indicates the activity and purity of the preparation. 

Deliverables

Proteins are aimed to be delivered on time and within specifications. If there are unpredictable hold ups we will keep you informed and work hard to get the project back on track.  Proteins are usually lyophilized for improved stability and this is included in the cost of the project. Products are usually packaged with two layers of desiccated protection to ensure stability, and a desiccating chamber is included for all custom orders.  The datasheet contains details of the proteins activity and purity, usually we will characterize the protein with 2 or 3 enzyme parameters e.g. Vmax, Kcat or Kd, and these parameters are developed in agreement with your expectations.  

Getting started

All we need from you is the protein's name, a quantity and timeline, send this information in an e-mail to tservice@cytoskeleton.com and we'll respond within 24 hours. We look forward to working with you to provide the required reagent in a suitable time frame.

 

Protein purification modules with deliverable and timelines:

Type

Name

 Deliverables

Example Protein

Amount

 Timeline (wk)

Mod. #

Recombinant protein

Small protein or protein domain (<30kDal) with gene provided by customer.

 Highly purified (>90% pure), E.coli expressed, His-tagged active protein lyophilized in 10 x 100 µg aliquots (or more depending on yield). Datasheet and assay method. Activity is in line with published articles.

RhoA small G-protein

10 x 100 µg

 4

REC012

Recombinant protein

Small protein or protein domain (<30kDal) with gene synthesis.

 Highly purified (>90% pure), E.coli expressed, His-tagged active protein lyophilized in 10 x 100 µg aliquots (or more depending on yield). Expression plasmid contianing gene. Datasheet and assay method. Activity is in line with published articles.

RhoA small G-protein

10 x 100 µg

 8

REC022

Recombinant protein

Medium to large protein or protein domain (31-100 kDal) with gene synthesis.

 Highly purified (>90% pure), E.coli expressed, His-tagged active protein lyophilized in 10 x 100 µg aliquots (or more depending on yield). Expression plasmid containing gene. Datasheet and assay method. Activity is in line with published articles.

CenPE kinesin

10 x 100 µg

 10

REC032

Recombinant protein

Small protein or protein domain (<30kDal) with gene synthesis and expression in eukaryotic system.

 Highly purified (>90% pure), CHO cell or Baculovirus/insect cell expressed, His-tagged active protein lyophilized in 10 x 100 µg aliquots (or more depending on yield). Expression plasmid containing gene. Datasheet and assay method. Activity is in line with published articles.

RhoA small G-protein with lipid tail

10 x 100 µg

 12

REC041

Recombinant protein

Medium to large protein or protein domain (31-100 kDal) with gene synthesis and expression in eukaryotic system.

 Highly purified (>90% pure), CHO cell or Baculovirus/insect cell expressed, His-tagged active protein lyophilized in 10 x 100 µg aliquots (or more depending on yield). Expression plasmid containing gene. Datasheet and assay method. Activity is in line with published articles.

Recombinant beta-Tubulin

10 x 100 µg

 12

REC042

Recombinant protein

Medium to large protein or protein domain (31-100 kDal) with multiple gene synthesis and co-expression in eukaryotic system. (useful for protein complex production).

 Highly purified (>90% pure), CHO cell or Baculovirus/insect cell expressed, His-tagged active protein lyophilized in 10 x 100 µg aliquots (or more depending on yield). Expression plasmid containing gene. Datasheet and assay method. Activity is in line with published articles.

Recombinant alpha/beta- Tubulin heterodimer

10 x 100 µg

 16

REC043

Native source protein

Cited protein purification

 Highly purified (>80% pure) protein from tissue or eukaryotic cells, lyophilized in 10 x 100 µg aliquots (or more depending on yield). Datasheet and assay method. Activity is in line with published articles.

Arp2/3 complex (seven protein complex)

10 x 100 µg

 4 to 20

REC052

 

If you have any questions please send an e-mail describing your requirements to tservice@cytoskeleton.com.