Customer Testimonials

 

 

Reviewed Product:  Anti-Phosphotyrosine Mouse Monoclonal Antibody
(Cat. # APY03)

Application: Western blot

“One of our lab’s primary focus is examining the signaling cascade of ouabain on the sodium pump, atp1a4.  We examine the broad effects of this cascade by looking at the phosphorylation patterns of proteins, specifically tyrosine-phosphorylated proteins.  In the past we used the anti-phosphotyrosine antibody, 4G10, from Millipore, a staple for these type of westerns.  When presented with an opportunity to try a different antibody, APY03, from your company, we were eager to see the results.  Overall, we were pleased with the consistent result when compared with the Millipore antibody.  In addition, the most significant advantage between the two antibodies was the huge savings in pricing.  With these two factors, reduction in cost and reliable results, we will continue to use your product and suggest it to others."

Jeff McDermott and Gladis Sanchez
University of Kansas Medical Center

 

   

Customer Results

 

 

 

Reviewed Product:  Anti-Phosphotyrosine Mouse Monoclonal Antibody
(Cat. # APY03)

Application: Western blot

“I have compared APY03 and 4G10 antibodies and I believe that APY03 is as good as 4G10 with the advantage of APY03 being that it is cheaper and also you get more western blots out of one purchase compared to 4G10.”

Felipe Navarrete, University of Massachusetts

   

Customer Results

Western blot analysis of extracts from non-capacitated (N. Cap) and capacitated sperm (Cap).  Cap extracts express a high level of proteins that are phosphorylated on tyrosine residues.  Manufacturers’ protocols followed.

 

Primary antibody dilution of APY03 was increased to 1:5000 and still produces a robust signal that detects phosphotyrosine residues undetected by 4G10.