Product Uses Include
Fibronectin purified from bovine plasma and has been modified to contain a covalently linked rhodamine fluorescent dye. An activated ester of rhodamine has been used to label the protein with a labeling stoichiometry of apprximately 1-3 dyes per protein molecule, a low labeling stiochiometry to retain functional activity. No free dye is apparent in the final product. Fibronectin has an approximate molecular weight of 250 kDa. FNR01 (20 µg of protein) is provided as a lyophilized powder.
Fluorescent Fibronectin Treated MCF10A cells
Fluorescent fibronectin (Cat. # FNR01) treated MCF10Acells (image kindly provided by A. Varadara and M. Karthykenyan, Univ. S.Carolina,Columbia, SC).
Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. Samples are >80% pure. No free dye is apparent in the final product.
Figure 1: Rhodamine Fibronectin Purity Determination. A 20 µg sample of rhodamine fibronectin (molecular weight approx. 250 kDa) was separated by electrophoresis oin a 4-20% SDS-PAGE system and stained with Coomassie Blue.
|Dickinson, Richard B. et al.||Viscous shaping of the compliant cell nucleus||APL Bioengineering||2022||ISSN 2473-2877|
|Van Der Putten, Cas et al.||Protein Micropatterning in 2.5D: An Approach to Investigate Cellular Responses in Multi-Cue Environments||ACS Applied Materials and Interfaces||2021||ISSN 1944-8252|
|Lauko, Domokos I. et al.||Baculovirus actin-rearrangement-inducing factor arif-1 induces the formation of dynamic invadosome clusters||Molecular Biology of the Cell||2021||ISSN 1939-4586|
|Garbett, Damien et al.||T-Plastin reinforces membrane protrusions to bridge matrix gaps during cell migration||Nature Communications||2020||ISSN 2041-1723|
|Naka, Y. et al.||Wholly vascularized millimeter-sized engineered tissues by cell-sized microscaffolds||Materials Today Bio||2020||ISSN 2590-0064|
|Summerbell, Emily R. et al.||Epigenetically heterogeneous tumor cells direct collective invasion through filopodia-driven fibronectin micropatterning||Science Advances||2020||ISSN 2375-2548|
|Sundararaman, Ananthalakshmy et al.||RhoJ Regulates α5β1 Integrin Trafficking to Control Fibronectin Remodeling during Angiogenesis||Current Biology||2020||ISSN 1879-0445|
|Lo Vecchio, Simon et al.||Collective Dynamics of Focal Adhesions Regulate Direction of Cell Motion||Cell Systems||2020||ISSN 2405-4720|
|Roveimiab, Ziba et al.||Traction and attraction: Haptotaxis substrates collagen and fibronectin interact with chemotaxis by HGF to regulate myoblast migration in a microfluidic device||American Journal of Physiology - Cell Physiology||2020||ISSN 1522-1563|
|Rafiq, Nisha Bte Mohd et al.||A mechano-signalling network linking microtubules, myosin IIA filaments and integrin-based adhesions||Nature Materials||2019||ISSN 1476-4660|
|Tomba, Caterina et al.||Laser-Assisted Strain Engineering of Thin Elastomer Films to Form Variable Wavy Substrates for Cell Culture||Small||2019||ISSN 1613-6829|
|Horvath, Aron N. et al.||The Protein Mat(ters) - Revealing the Biologically Relevant Mechanical Contribution of Collagen- And Fibronectin-Coated Micropatterns||ACS Applied Materials and Interfaces||2019||ISSN 1944-8252|
|Hasan, Muhammad M. et al.||Invadosome-mediated human extracellular matrix degradation by Entamoeba histolytica||Infection and Immunity||2018||ISSN 1098-5522|
|De Vos, Ivo J.H.M. et al.||Functional analysis of a hypomorphic allele shows that MMP14 catalytic activity is the prime determinant of the Winchester syndrome phenotype||Human Molecular Genetics||2018||ISSN 1460-2083|
|Varadaraj, Archana et al.||TGF-β triggers rapid fibrillogenesis via a novel TβRII-dependent fibronectin-trafficking mechanism||Molecular Biology of the Cell||2017||ISSN 1939-4586|
|Funano, Shun Ichi et al.||Vapor-based micro/nano-partitioning of fluoro-functional group immobilization for long-term stable cell patterning||RSC Advances||2016||ISSN 2046-2069|
|Mana, Giulia et al.||PPFIA1 drives active α5β1 integrin recycling and controls fibronectin fibrillogenesis and vascular morphogenesis||Nature Communications||2016||ISSN 2041-1723|
|Blehm, Benjamin H. et al.||Deconstructing the role of the ECM microenvironment on drug efficacy targeting MAPK signaling in a pre-clinical platform for cutaneous melanoma||Biomaterials||2015||ISSN 1878-5905|
|Wood, Sheila||Differentiation of Borrelia Microbes from Collagen Debris and Collagen Fibrils in Blood Cultures||Journal of Microbiology & Experimentation||2015||Article Link|
|Comelles, Jordi et al.||Cells as Active Particles in Asymmetric Potentials: Motility under External Gradients||Biophysical Journal||2014||ISSN 1542-0086|
|Nakayama, Masamichi et al.||Thermoresponsive poly(N-isopropylacrylamide)-based block copolymer coating for optimizing cell sheet fabrication||Macromolecular bioscience||2012||ISSN 1616--5195|
|Steele, Amanda N. et al.||Tandem zyxin LIM sequences do not enhance force sensitive accumulation||Biochemical and Biophysical Research Communications||2012||ISSN 0006-291X|
|Nagase, Kenichi et al.||Thermo-responsive polymer brushes as intelligent biointerfaces: preparation via ATRP and characterization||Macromolecular bioscience||2011||ISSN 1616--5195|
|Steward, Robert L. et al.||Mechanical stretch and shear flow induced reorganization and recruitment of fibronectin in fibroblasts||Scientific Reports||2011||ISSN 2045-2322|
|Robinson, Elizabeth E. et al.||Fibronectin Matrix Assembly Regulates α5β1-mediated Cell Cohesion||Molecular Biology of the Cell||2004||ISSN 1059-1524|
|Brock, Amy et al.||Geometric Determinants of Directional Cell Motility Revealed Using Microcontact Printing†||Langmuir||2003||ISSN 0743-7463|
Question 1: What is the optimal excitation and emission filter settings to visualize the rhodamine fluorescence?
Answer 1: Rhodamine fibronectin can be detected using a filter set of 535 nm excitation and 585 nm emission.
Question 2: What is the labeling stoichiometry?
Answer 2: Rhodamine labeling stoichiometry was calculated to be 1-3 dyes per fibronectin protein using the absorbance maximum for rhodamine at 565 nm and the Beer-Lambert law. Dye extinction coefficient when protein bound is 70,000 M-1cm-1.
If you have any questions concerning this product, please contact our Technical Service department at firstname.lastname@example.org