Rhodamine fibronectin

Rhodamine fibronectin

Product Uses Include

  • Tracking cell movement through a 3D matrix
  • Tracking cell movement across a glass slide
  • Observation of fibronectin matrix assembly and cell adhesion.
  • FACS analysis of fibronectin binding cells

Fibronectin purified from bovine plasma and has been modified to contain a covalently linked rhodamine fluorescent dye. An activated ester of rhodamine has been used to label the protein with a labeling stoichiometry of apprximately 1-3 dyes per protein molecule, a low labeling stiochiometry to retain functional activity. No free dye is apparent in the final product. Fibronectin has an approximate molecular weight of 250 kDa. FNR01 (20 µg of protein) is provided as a lyophilized powder.

Fluorescent Fibronectin Treated MCF10A cells


Fluorescent fibronectin (Cat. # FNR01) treated MCF10Acells (image kindly provided by A. Varadara and M. Karthykenyan, Univ. S.Carolina,Columbia, SC).

Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. Samples are >80% pure. No free dye is apparent in the final product. 


Figure 1: Rhodamine Fibronectin Purity Determination. A 20 µg sample of rhodamine fibronectin (molecular weight approx. 250 kDa) was separated by electrophoresis oin a 4-20% SDS-PAGE system and stained with Coomassie Blue.

For product Datasheets and MSDSs please click on the PDF links below.   For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com

AuthorTitleJournalYearArticle Link
Dickinson, Richard B. et al.Viscous shaping of the compliant cell nucleusAPL Bioengineering2022ISSN 2473-2877
Van Der Putten, Cas et al.Protein Micropatterning in 2.5D: An Approach to Investigate Cellular Responses in Multi-Cue EnvironmentsACS Applied Materials and Interfaces2021ISSN 1944-8252
Lauko, Domokos I. et al.Baculovirus actin-rearrangement-inducing factor arif-1 induces the formation of dynamic invadosome clustersMolecular Biology of the Cell2021ISSN 1939-4586
Garbett, Damien et al.T-Plastin reinforces membrane protrusions to bridge matrix gaps during cell migrationNature Communications2020ISSN 2041-1723
Naka, Y. et al.Wholly vascularized millimeter-sized engineered tissues by cell-sized microscaffoldsMaterials Today Bio2020ISSN 2590-0064
Summerbell, Emily R. et al.Epigenetically heterogeneous tumor cells direct collective invasion through filopodia-driven fibronectin micropatterningScience Advances2020ISSN 2375-2548
Sundararaman, Ananthalakshmy et al.RhoJ Regulates α5β1 Integrin Trafficking to Control Fibronectin Remodeling during AngiogenesisCurrent Biology2020ISSN 1879-0445
Lo Vecchio, Simon et al.Collective Dynamics of Focal Adhesions Regulate Direction of Cell MotionCell Systems2020ISSN 2405-4720
Roveimiab, Ziba et al.Traction and attraction: Haptotaxis substrates collagen and fibronectin interact with chemotaxis by HGF to regulate myoblast migration in a microfluidic deviceAmerican Journal of Physiology - Cell Physiology2020ISSN 1522-1563
Rafiq, Nisha Bte Mohd et al.A mechano-signalling network linking microtubules, myosin IIA filaments and integrin-based adhesionsNature Materials2019ISSN 1476-4660
Tomba, Caterina et al.Laser-Assisted Strain Engineering of Thin Elastomer Films to Form Variable Wavy Substrates for Cell CultureSmall2019ISSN 1613-6829
Horvath, Aron N. et al.The Protein Mat(ters) - Revealing the Biologically Relevant Mechanical Contribution of Collagen- And Fibronectin-Coated MicropatternsACS Applied Materials and Interfaces2019ISSN 1944-8252
Hasan, Muhammad M. et al.Invadosome-mediated human extracellular matrix degradation by Entamoeba histolyticaInfection and Immunity2018ISSN 1098-5522
De Vos, Ivo J.H.M. et al.Functional analysis of a hypomorphic allele shows that MMP14 catalytic activity is the prime determinant of the Winchester syndrome phenotypeHuman Molecular Genetics2018ISSN 1460-2083
Varadaraj, Archana et al.TGF-β triggers rapid fibrillogenesis via a novel TβRII-dependent fibronectin-trafficking mechanismMolecular Biology of the Cell2017ISSN 1939-4586
Funano, Shun Ichi et al.Vapor-based micro/nano-partitioning of fluoro-functional group immobilization for long-term stable cell patterningRSC Advances2016ISSN 2046-2069
Mana, Giulia et al.PPFIA1 drives active α5β1 integrin recycling and controls fibronectin fibrillogenesis and vascular morphogenesisNature Communications2016ISSN 2041-1723
Blehm, Benjamin H. et al.Deconstructing the role of the ECM microenvironment on drug efficacy targeting MAPK signaling in a pre-clinical platform for cutaneous melanomaBiomaterials2015ISSN 1878-5905
Wood, SheilaDifferentiation of Borrelia Microbes from Collagen Debris and Collagen Fibrils in Blood CulturesJournal of Microbiology & Experimentation2015Article Link
Comelles, Jordi et al.Cells as Active Particles in Asymmetric Potentials: Motility under External GradientsBiophysical Journal2014ISSN 1542-0086
Nakayama, Masamichi et al.Thermoresponsive poly(N-isopropylacrylamide)-based block copolymer coating for optimizing cell sheet fabricationMacromolecular bioscience2012ISSN 1616--5195
Steele, Amanda N. et al.Tandem zyxin LIM sequences do not enhance force sensitive accumulationBiochemical and Biophysical Research Communications2012ISSN 0006-291X
Nagase, Kenichi et al.Thermo-responsive polymer brushes as intelligent biointerfaces: preparation via ATRP and characterizationMacromolecular bioscience2011ISSN 1616--5195
Steward, Robert L. et al.Mechanical stretch and shear flow induced reorganization and recruitment of fibronectin in fibroblastsScientific Reports2011ISSN 2045-2322
Robinson, Elizabeth E. et al.Fibronectin Matrix Assembly Regulates α5β1-mediated Cell CohesionMolecular Biology of the Cell2004ISSN 1059-1524
Brock, Amy et al.Geometric Determinants of Directional Cell Motility Revealed Using Microcontact Printing†Langmuir2003ISSN 0743-7463


Question 1:  What is the optimal excitation and emission filter settings to visualize the rhodamine fluorescence?

Answer 1:  Rhodamine fibronectin can be detected using a filter set of 535 nm excitation and 585 nm emission.


Question 2:  What is the labeling stoichiometry?

Answer 2:  Rhodamine labeling stoichiometry was calculated to be 1-3 dyes per fibronectin protein using the absorbance maximum for rhodamine at 565 nm and the Beer-Lambert law. Dye extinction coefficient when protein bound is 70,000 M-1cm-1.



If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com