• Substrate for fungal microtubule (MT) activated kinesin KIF11 ATPase assay
• Comparison between human and fungal KIF11 homologs

BimC, also known as the human Eg5 (KIF11) homolog, is a slow, plus-end–directed motor protein essential for separating duplicated spindle pole bodies and assembling a bipolar mitotic spindle

The motor domain of Aspergillus fumigatus BimC kinesin is produced in a bacterial expression system.

• Protein tag: N-terminal 6x His-tag
• Molecular weight: 50 kDa
• Formulation: supplied as lyophilized powder
• Composition: when reconstituted 80 mM PIPES pH 6.8, 100 mM NaCl, 2 mM MgCl2, 5% (w/v) sucrose, and 1% (w/v) dextran

Protein purity is assessed by scanning densitometry of Coomassie Blue-stained protein on a4-20% polyacrylamide gel. Purity is determined to be >90% pure.

Biological activity of EG02 is measured using a microtubule-activated ATPase assay. Under the conditions stated (see datasheet), EG02 exhibits a microtubule-stimulated ATPase activity with a Vmax of ≥ 250 nmol of ATP produced per minute per mg of KIF11.