Rhodamine fibronectin

Rhodamine fibronectin
$0.00

Product Uses Include

  • Tracking cell movement through a 3D matrix
  • Tracking cell movement across a glass slide
  • Observation of fibronectin matrix assembly and cell adhesion.
  • FACS analysis of fibronectin binding cells

Material
Fibronectin purified from bovine plasma and has been modified to contain a covalently linked rhodamine fluorescent dye. An activated ester of rhodamine has been used to label the protein with a labeling stoichiometry of apprximately 1-3 dyes per protein molecule, a low labeling stiochiometry to retain functional activity. No free dye is apparent in the final product. Fibronectin has an approximate molecular weight of 250 kDa. FNR01 (20 µg of protein) is provided as a lyophilized powder.

Fluorescent Fibronectin Treated MCF10A cells

Fluorescent_fibronectin_overlay_with_DIC_v3b

Fluorescent fibronectin (Cat. # FNR01) treated MCF10Acells (image kindly provided by A. Varadara and M. Karthykenyan, Univ. S.Carolina,Columbia, SC).

Purity
Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. Samples are >80% pure. No free dye is apparent in the final product. 

fnr01gel


Figure 1: Rhodamine Fibronectin Purity Determination. A 20 µg sample of rhodamine fibronectin (molecular weight approx. 250 kDa) was separated by electrophoresis oin a 4-20% SDS-PAGE system and stained with Coomassie Blue.


For product Datasheets and MSDSs please click on the PDF links below.   For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com

AuthorTitleJournalYearArticle Link
Lacroix, Mathilde et al.Emergence of bidirectional cell laning from collective contact guidanceNature Physics2024
Lo Vecchio, S. et al.Spontaneous rotations in epithelia as an interplay between cell polarity and boundariesNature Physics2024
Stinson, Matthew W. et al.Macrophage migration is differentially regulated by fibronectin and laminin through altered adhesion and myosin II localizationMolecular Biology of the Cell2024
Lichtenberg, Jessanne Y. et al.Leader cells mechanically respond to aligned collagen architecture to direct collective migrationPLOS ONE2024
Caillier, Alexia et al.T cells use focal adhesions to pull themselves through confined environmentsJournal of Cell Biology2024
Jana, Aniket et al.Mitotic outcomes and errors in fibrous environmentsProceedings of the National Academy of Sciences2023
Graybill, Philip M. et al.Ultra-thin and ultra-porous nanofiber networks as a basement-membrane mimicLab on a Chip2023
Sakai, Koji et al.Small-artery-mimicking multi-layered 3D co-culture in a self-folding porous graphene-based filmNanoscale Horizons2023
Mukherjee, Apratim et al.Actin Filaments Couple the Protrusive Tips to the Nucleus through the I-BAR Domain Protein IRSp53 during the Migration of Cells on 1D FibersAdvanced Science2023
Comelles, Jordi et al.Soft topographical patterns trigger a stiffness-dependent cellular response to contact guidanceMaterials Today Bio2023
Sadhu, Raj Kumar et al.Experimental and theoretical model for the origin of coiling of cellular protrusions around fibersNature Communications2023
Gibson, Shayin V. et al.ADAMTS3 restricts cancer invasion in models of early breast cancer progression through enhanced fibronectin degradationMatrix Biology2023
Dickinson, Richard B. et al.Viscous shaping of the compliant cell nucleusAPL Bioengineering2022
Van Der Putten, Cas et al.Protein Micropatterning in 2.5D: An Approach to Investigate Cellular Responses in Multi-Cue EnvironmentsACS Applied Materials and Interfaces2021
Lauko, Domokos I. et al.Baculovirus actin-rearrangement-inducing factor arif-1 induces the formation of dynamic invadosome clustersMolecular Biology of the Cell2021
Naka, Y. et al.Wholly vascularized millimeter-sized engineered tissues by cell-sized microscaffoldsMaterials Today Bio2020
Summerbell, Emily R. et al.Epigenetically heterogeneous tumor cells direct collective invasion through filopodia-driven fibronectin micropatterningScience Advances2020
Sundararaman, Ananthalakshmy et al.RhoJ Regulates α5β1 Integrin Trafficking to Control Fibronectin Remodeling during AngiogenesisCurrent Biology2020
Lo Vecchio, Simon et al.Collective Dynamics of Focal Adhesions Regulate Direction of Cell MotionCell Systems2020
Garbett, Damien et al.T-Plastin reinforces membrane protrusions to bridge matrix gaps during cell migrationNature Communications2020
Roveimiab, Ziba et al.Traction and attraction: Haptotaxis substrates collagen and fibronectin interact with chemotaxis by HGF to regulate myoblast migration in a microfluidic deviceAmerican Journal of Physiology - Cell Physiology2020
Horvath, Aron N. et al.The Protein Mat(ters) - Revealing the Biologically Relevant Mechanical Contribution of Collagen- And Fibronectin-Coated MicropatternsACS Applied Materials and Interfaces2019
Tomba, Caterina et al.Laser-Assisted Strain Engineering of Thin Elastomer Films to Form Variable Wavy Substrates for Cell CultureSmall2019
Rafiq, Nisha Bte Mohd et al.A mechano-signalling network linking microtubules, myosin IIA filaments and integrin-based adhesionsNature Materials2019
De Vos, Ivo J.H.M. et al.Functional analysis of a hypomorphic allele shows that MMP14 catalytic activity is the prime determinant of the Winchester syndrome phenotypeHuman Molecular Genetics2018
Hasan, Muhammad M. et al.Invadosome-mediated human extracellular matrix degradation by Entamoeba histolyticaInfection and Immunity2018
Varadaraj, Archana et al.TGF-β triggers rapid fibrillogenesis via a novel TβRII-dependent fibronectin-trafficking mechanismMolecular Biology of the Cell2017
Funano, Shun Ichi et al.Vapor-based micro/nano-partitioning of fluoro-functional group immobilization for long-term stable cell patterningRSC Advances2016
Mana, Giulia et al.PPFIA1 drives active α5β1 integrin recycling and controls fibronectin fibrillogenesis and vascular morphogenesisNature Communications2016
Blehm, Benjamin H. et al.Deconstructing the role of the ECM microenvironment on drug efficacy targeting MAPK signaling in a pre-clinical platform for cutaneous melanomaBiomaterials2015
Wood, SheilaDifferentiation of Borrelia Microbes from Collagen Debris and Collagen Fibrils in Blood CulturesJournal of Microbiology & Experimentation2015
Comelles, Jordi et al.Cells as Active Particles in Asymmetric Potentials: Motility under External GradientsBiophysical Journal2014
Steele, Amanda N. et al.Tandem zyxin LIM sequences do not enhance force sensitive accumulationBiochemical and Biophysical Research Communications2012
Nakayama, Masamichi et al.Thermoresponsive poly(N-isopropylacrylamide)-based block copolymer coating for optimizing cell sheet fabricationMacromolecular bioscience2012
Nagase, Kenichi et al.Thermo-responsive polymer brushes as intelligent biointerfaces: preparation via ATRP and characterizationMacromolecular bioscience2011
Steward, Robert L. et al.Mechanical stretch and shear flow induced reorganization and recruitment of fibronectin in fibroblastsScientific Reports2011
Robinson, Elizabeth E. et al.Fibronectin Matrix Assembly Regulates α5β1-mediated Cell CohesionMolecular Biology of the Cell2004
Brock, Amy et al.Geometric Determinants of Directional Cell Motility Revealed Using Microcontact Printing†Langmuir2003

 

Question 1:  What is the optimal excitation and emission filter settings to visualize the rhodamine fluorescence?

Answer 1:  Rhodamine fibronectin can be detected using a filter set of 535 nm excitation and 585 nm emission.

 

Question 2:  What is the labeling stoichiometry?

Answer 2:  Rhodamine labeling stoichiometry was calculated to be 1-3 dyes per fibronectin protein using the absorbance maximum for rhodamine at 565 nm and the Beer-Lambert law. Dye extinction coefficient when protein bound is 70,000 M-1cm-1.

 

 

If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com