Gelsolin protein: Homo sapiens recombinant

Gelsolin protein: Homo sapiens recombinant

Product Uses Include

  • Positive control for studying the activity of F-actin severing and capping proteins
  • Investigation of the effect of actin binding proteins (ABPs) on actin dynamics

Gelsolin protein has been purified from E.coli expressing recombinant 6xHIS-tagged human gelsolin (plasma isoform). Gelsolin belongs to a class of actin severing and capping proteins called class I F-actin capping proteins. Each of these class I proteins contains a series of conserved 125-150 amino acid repeat motifs. Gelsolin is characterized by the presence of six repeated motifs, three of which are actin binding domains. Gelsolin exerts a powerful regulatory role on actin filament length and its activity can be modulated by Ca2+ levels, pH , polyphosphoinositides and post-translational modification. Plasma gelsolin contains an additional 25aa leader sequence compared to the cytoplasmic isoform. Gelsolin is supplied as a white lyophilized powder. After reconstitution with of nanopure water, the protein will be in the following buffer: 10 mM Tris pH 7.5, 10 mM NaCl, 0.1 mM MgCl2, 1% (w/v) sucrose, and 0.1% (w/v) dextran. The lyophilized protein is stable at 4°C desiccated (<10% humidity) for 6 months.

Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 4-20% gradient polyacrylamide gel. Gelsolin protein is >90% pure (see Figure 1) and runs at approximately 95 kDa (due to leader sequence and 6xHIS-tag).


Figure 1. Gelsolin protein purity determination. A 20 µg sample of HPG5 was separated by electrophoresis in a 4-20% SDS-PAGE system and stained with Coomassie Blue. Protein quantitation was performed using the Precision Red Protein Assay Reagent (Cat.# ADV02).

Biological Activity
The biological activity of gelsolin is determined in an F-actin severing assay. F-actin is incubated with gelsolin and the reaction products are centrifuged at 100,000 x g for 1 h. The proportion of actin in the supernatant (G-actin) versus the pellet (F-actin) is compared to a control reaction without gelsolin. Stringent quality control ensures that gelsolin (2 µg) can solubilize 70-80% of F-actin (5 µg) in five min (see Figure 2)


Figure 2. Gelsolin biological activity determination. Sample 1 - Control F-actin without gelsolin. Sample 2 - F-actin with 2µg of gelsolin. Sample 3 - 2µg of gelsolin only. S = supernatant, P = pellet.

For product Datasheets and MSDSs please click on the PDF links below.   For additional information, click on the FAQs tab above or contact our Technical Support department at

AuthorTitleJournalYearArticle Link
Giampazolias, Evangelos et al.Secreted gelsolin inhibits DNGR-1-dependent cross-presentation and cancer immunityCell2021ISSN 1097-4172
Kučera, Ondřej et al.Anillin propels myosin-independent constriction of actin ringsNature Communications2021ISSN 2041-1723
Clement, Cristina C. et al.The dendritic cell Major Histocompatibility Complex II (MHC II) peptidome derives from a variety of processing pathways and includes peptides with a broad spectrum of HLA-DM sensitivityJournal of Biological Chemistry2016ISSN 1083-351X
Gurmessa, Bekele et al.Entanglement Density Tunes Microscale Nonlinear Response of Entangled ActinMacromolecules2016ISSN 1520-5835
Cantero, María Del Rocío et al.Polycystin-2 (TRPP2) regulation by Ca2+ is effected and diversified by actin-binding proteinsBiophysical Journal2015ISSN 1542-0086

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