• Substrate for microtubule (MT) activated kinesin ATPase assay
• Screen for KIF3C (kinesin-2) inhibitors
• Counter-screen for kinesin drug candidates

KIF3C is a motor protein belonging to the kinesin-2 family, involved in microtubule-based transport of cargo within cells, particularly in neurons. It plays a crucial role in intracellular trafficking, signaling, and the maintenance of axonal and dendritic structures.

The wild-type human motor domain of KIF3C is produced in a bacterial expression system.

• Protein tag: N-terminal GST tag
• Molecular weight: 73 kDa
• Motor amino acids: 2-428
• Uniprot ID: O14782
• Formulation: supplied as lyophilized powder
• Composition: when reconstituted 80 mM PIPES pH 6.8, 100 mM NaCl, 2 mM MgCl2, 5% (w/v) sucrose, and 1% (w/v) dextran

Protein purity is assessed by scanning densitometry of Coomassie Blue stained protein on a 4-20% polyacrylamide gel. Purity is determined to be >80% pure.

Biological activity of KIF3C is measured using a microtubule-activated ATPase assay. Under the conditions stated (see datasheet), KIF3C exhibits a microtubule-stimulated ATPase activity with a Vmax of ≥ 50 nmol of ATP produced per minute per mg of KIF3C.