Application of MemGlow™ probes to neurobiological studies
(A MemBright™ family member of probes and dyes)
Advantages of MemGlow™ for neuronal studies
- No transfections or transgenic animals required for neuronal labeling
- Non-toxic probes enable long-term investigations
- Broad spectral variety ideal for co-labeling studies
- High-fidelity visualization of neuronal protrusions in live cells with confocal
- MemGlow™ 590 is compatible with super resolution (STORM) and confocal
Hippocampal astrocytic filopodia are efficiently labeled with MemGlow™ 560.
Figure 1: Hippocampal astrocytic projections are clearly and efficiently labeled
with 20 nM MemGlow™ 560 when visualized with confocal microscopy. Image
adapted from Collot, M. et al. 2019.
High fidelity images of neurons and synapses
Figure 2; Hippocampal neurons are preferentially labeled by MemGlow™ 560 over
neighboring glial cells. Image adapted from Collot, M. et al. 2019.
MemGlow™ 590 has been validated for use in Stochastic Optical Reconstruction Microscopy (STORM).
Unravel the dendritic spine morphology of neurons with MemGlow™ 590 and gain
insights into minute structural changes elicited by your treatments.
Hippocampal neurons labeled with MemGlow™ 590 after 9 days in vitro and visualized using STORM
microscopy. Imaging reveals neuronal protrustions at sub-diffraction-limit resolution (A2)
after locating with wide-field (A1). Image adapted from Collot, M. et al. 2019.
Hippocampal neurons laying over an astrocytic layer
Live cultured hippocampal neurons laying over an astrocytic layer are brilliantly labeled with 20 nM
MemGlow™ 590 (A) and MemGlow™ 700 (B) and 5 μg/ml Hoechst. Image from
Collot, M. et al. 2019.
General features and advantages of MemGlow™ probes:
- Bright and fluorogenic
- Do not alter sample biology
- Simple staining protocol and low working concentration
- Compatible with live and fixed cell staining
- Compatible with ex vivo and fixed tissue staining
- MemGlow™ 590 is super-resolution compatible
- Superior to many existing plasma membrane dyes
- No dye quenching steps required
- Non-toxic dyes for long-term imaging of live cells
- Utilize cyanine or BODIPY dyes with zwitterionic membrane anchor groups
Fluorogenic MemGlow™ probes are poised to change neurobiology studies!
Selected Resources:
Learn more about MemGlow™ Click Here
View a mechanism of the diagram Click Here
For an in-depth look at MemGlow™ probes Click Here
| Product | Excitation/Emission (nm) | Filter |
| MemGlow 488 | 499/507 | FITC / GFP |
| MemGlow 560 | 555/570 | CY 3 / TRITC |
| MemGlow 590 | 595/613 | CY 3.5 / Texas Red |
| MemGlow 640 | 650/673 | Cy 5 |
| MemGlow 700 | 689/713 | Cy 5.5 |