• Substrate for microtubule (MT) activated kinesin ATPase assay
• Screen for KIF2C (MCAK, kinesin-13A) inhibitors
• Counter-screen for kinesin drug candidates

Human KIF2C (MCAK) is a microtubule depolymerizing motor protein essential for regulating spindle dynamics and chromosome alignment during mitosis. By controlling microtubule length at kinetochores, KIF2C ensures accurate chromosome segregation and genomic stability.

The wild-type human motor domain of KIF5B is produced in a bacterial expression system.

• Protein tag: N-terminal GST tag
• Molecular weight: 79 kDa
• Motor amino acids: 182-646
• Uniprot ID: Q99661
• Formulation: supplied as lyophilized powder
• Composition: when reconstituted 80 mM PIPES pH 6.8, 100 mM NaCl, 2 mM MgCl2, 5% (w/v) sucrose, and 1% (w/v) dextran

Protein purity is assessed by scanning densitometry of Coomassie Blue-stained protein on a4-20% polyacrylamide gel. Purity is determined to be >80% pure.

Biological activity of KIF2C is measured using a microtubule-activated ATPase assay. Under the conditions stated (see datasheet), KIF2C exhibits a microtubule-stimulated ATPase activity with a Vmax of ≥ 160 nmol of ATP produced per minute per mg of KIF2C.