• Substrate for microtubule (MT) activated kinesin ATPase assay
• Screen for KIF20A inhibitors
• Counter-screen for kinesin drug candidates

Human KIF20A is a mitotic kinesin motor protein essential for proper chromosome segregation and cytokinesis, functioning by interacting with microtubules to drive spindle organization. It plays a key role in cell cycle progression, and its dysregulation has been linked to cancer proliferation and poor prognosis.

The wild-type human motor domain of KIF20A (MKLP2) is produced in a bacterial expression system.

• Protein tag: N-terminal 6x His tag
• Molecular weight: 54 kDa
• Motor amino acids: 1-519
• Uniprot ID: O95235
• Formulation: supplied as lyophilized powder
• Composition: when reconstituted 80 mM PIPES pH 6.8, 100 mM NaCl, 2 mM MgCl2, 0.2% Triton X-100, 6% (w/v) sucrose, and 1% (w/v) dextran

Protein purity is assessed by scanning densitometry of Coomassie Blue-stained protein on a4-20% polyacrylamide gel. Purity is determined to be >70% pure.

Biological activity of CS-KF20A is measured using a microtubule-activated ATPase assay. Under the conditions stated (see datasheet), KIF20A exhibits a microtubule-stimulated ATPase activity with a Vmax of ≥ 1330 nmol of ATP produced per minute per mg of KIF20A.