• Substrate for microtubule (MT) activated kinesin ATPase assay
• Screen for KIF23 (MKLP1, kinesin-6) inhibitors
• Counter-screen for kinesin drug candidates

KIF23 (MKLP1) is a human kinesin motor protein essential for cytokinesis, where it drives the final separation of daughter cells by organizing and stabilizing the central spindle. It plays a critical role in cell division and is implicated in cancer progression when overexpressed or dysregulated.

The wild-type human motor domain of KIF23 is produced in a bacterial expression system.

• Protein tag: N-terminal GST tag
• Molecular weight: 74 kDa
• Motor amino acids: 17-502
• Uniprot ID: Q02241
• Formulation: supplied as lyophilized powder
• Composition: when reconstituted 80 mM PIPES pH 6.8, 100 mM NaCl, 2 mM MgCl2, 5% (w/v) sucrose, and 1% (w/v) dextran

Protein purity is assessed by scanning densitometry of Coomassie Blue-stained protein on a4-20% polyacrylamide gel. Purity is determined to be >70% pure.

Biological activity of KIF23 is measured using a microtubule-activated ATPase assay. Under the conditions stated (see datasheet), KIF23 exhibits a microtubule-stimulated ATPase activity with a Vmax of ≥ 600 nmol of ATP produced per minute per mg of KIF23.