Protease inhibitor cocktail

Protease inhibitor cocktail
$0.00

Product Uses Include

  • Inhibition of a wide range of proteases.
  • Popular Activation Assay Component

Material
Protease Inhibitor Cocktail is supplied as a white lyophilized powder. The cocktail contains the following protease inhibitors, Pepstatin A, Leupeptin, Benzamidine, Na-p-tosyl-L-arginine methyl ester (TAME). At 1X final working concentration the inhibitors are at 1 µM, 1.5 µM, 1 mM and 0.4 mM respectively.

Mode of Action
pepstatin A Reversibly inhibits some aspartic proteases
Leupeptin Reversibly inhibits trypsin like serine and some cysteine proteases
Benzamidine Inhibits serine proteases
TAME Inhibits arginine proteases, a thrombin and trypsin inhibitor

Storage and Reconstitution
Lyophilized Protease Inhibitor Cocktail is stable at 4°C desiccated (<10% humidity) for 1 year.
Each tube of Protease Inhibitor Cocktail should be resuspended in 1ml of DMSO (dimethyl sulfoxide) to give a 100X strength stock. The reconstituted stock can be stored at 4°C for up to 6 months or at -70°C for up to 1 year.

Related Products
This protease inhibitor cocktail is used in the following Cytoskeleton Activation Assays:

  • Ras activation assay Biochem kit (Cat. # BK008)
  • Cdc42 activation assay Biochem kit (Cat. # BK034)
  • Rac activation assay Biochem kit (Cat. # BK035)
  • RhoA activation assay Biochem kit (Cat. # BK036)
  • G-actin : F-actin in vivo assay Biochem kit (Cat. # BK037)
  • Microtubule : tubulin in vivo assay Biochem kit (Cat. # BK038)
  • RhoA G-LISA™ Activation Assay Kit (luminescence) (Cat. # BK121)
  • RhoA G-LISA™ Activation Assay Kit (colorimetric) (Cat. # BK124)
  • Rac1,2,3 G-LISA™ Activation Assay Kit (colorimetric) (Cat. # BK125)
  • Rac1 G-LISA™ Activation Assay Kit (luminescence) (Cat. # BK126)
  • Cdc42 G-LISA™ Activation Assay (Cat. # BK127)
  • Rac1 G-LISA™ Activation Assay (Colorimetric Based) (Cat. # BK128)
  • RalA G-LISA™ Activation Assay (Colorimetric Based) (Cat. # BK129)

For product Datasheets and MSDSs please click on the PDF links below.   For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com

V. Munnamalai et al. 2014. Bidirectional interactions between NOX2-type NADPH oxidase and the F-actin cytoskeleton in neuronal growth cones. J. Neurochem. doi: 10.1111/jnc.12734.

H. Vindin et al. 2013. Validation of an algorithm to quantify changes in actin cytoskeletal organization. J. Biomol. Screen. 19, 354-68.

D. Chand et al. 2012. C-terminal region of teneurin-1 co-localizes with dystroglycan and modulates cytoskeletal organization through an extracellular signal-regulated kinase-dependent stathmin- and filamin A-mediated mechanism in hippocampal cells. Neurosci. 219, 255–270.

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Fernandez, Christian et al.TMPRSS11a is a novel age-altered, tissue specific regulator of migration and wound healingFASEB Journal2021ISSN 1530-6860
Berganza, Eider et al.A multiplexed phospholipid membrane platform for curvature sensitive protein screeningNanoscale2021ISSN 2040-3372
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Park, Jin Suk et al.Mechanical regulation of glycolysis via cytoskeleton architectureNature2020ISSN 1476-4687
Rivas, José et al.KCTD5, a novel TRPM4-regulatory protein required for cell migration as a new predictor for breast cancer prognosisFASEB Journal2020ISSN 1530-6860
Gu, Chunming et al.Identification of berberine as a novel drug for the treatment of multiple myeloma via targeting UHRF1BMC Biology2020ISSN 1741-7007
Dér, Bálint et al.NK2 receptor-mediated detrusor muscle contraction involves Gq/11-dependent activation of voltage-dependent Ca2+ channels and the RhoA-Rho kinase pathwayAmerican Journal of Physiology - Renal Physiology2019ISSN 1522-1466
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Blanco, Constanza et al.EB1- and EB2-dependent anterograde trafficking of TRPM4 regulates focal adhesion turnover and cell invasionFASEB Journal2019ISSN 1530-6860
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Weise-Cross, Laura et al.Actin polymerization contributes to enhanced pulmonary vasoconstrictor reactivity after chronic hypoxiaAmerican Journal of Physiology - Heart and Circulatory Physiology2018ISSN 1522-1539
Riquelme, Denise et al.Subcellular localization and activity of TRPM4 in medial prefrontal cortex layer 2/3Frontiers in Cellular Neuroscience2018ISSN 1662-5102
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