eFM488 Enhanced Fluorogenic Membrane Probe

• Fluorogenic plasma membrane probe for live or fixed cells and tissues
• Efficient labeling of plant plasma membranes
• Label extracellular vesicles
• Suitable for primary neuron imaging
• Multichannel imaging

The eFM plasma membrane probe eFM488 is an advanced version of the widely used FM probes.

Key features

• Ab: 488 nm, Em: 515-600 nm-green/orange spectrum
• Enhanced fluorogenic nature compared to FM probes
• Significantly lower endosomal internalization compared to FM probes
• Offers high specificity for outer membrane staining
• Extremely low background
• Enhanced retention at plasma membrane
• Validated in multiple microscopy techniques including STED
• Labels live and fixed cells and tissues
• Large Stokes shift simplifies multicolor flourescence imaging

The biological activity of MG15 is assessed by the ability of the probe to efficiently label plasma membranes in live HEK293 cell culture.