• High resolution imaging of the actin cytoskeleton by expansion microscopy
• Multiplex imaging alongside other fluorescent markers

HAK-actin™ is a pan-species Expansion Microscopy (ExM) compatible probe for actin visualization using Ultrastructure ExM (U-ExM), Iterative U-ExM (iU-ExM) and Cryo-ExM based protocols.

Key features

• Stronger post-expansion signal: HAK-actin™ includes an HA-tag, enabling post-ExM labeling with standard anti-HA antibodies, delivering ≈4x higher fluorescence than directly labeled probes
• Exceptional retention in the expansion gel: Four lysines promote efficient crosslinking to the expansion polymer, producing bright specific actin labeling with low background
• Labels endogenous actin: No transfection, expression, or genetic manipulation required
• Broad Applicability across models: Validated across diverse systems, including human cultured cells, neurons, microbial eukaryotes (e.g., yeasts)

HAK-actin™ has been specifically validated from U-ExM, iU-ExM and Cryo-EM protocols.