• Cell invasion assays
• FACS analysis of laminin-binding cells

Laminin is purified from Engelbreth-Holm-Swarm (EHS) mouse tumor tissue; it is a high-molecular-weight (~800 kDa) heterotrimeric basement membrane glycoprotein, made up of three subunits that vary in size between 400 kDa (alpha subunit) and 200-225 kDa (beta and gamma subunits).

The protein is modified to contain covalently linked rhodamines at random surface lysines.

• Ab: 565nm Em: 585 nm – orange/red spectrum
• Labeling stoichiometry: ~5-5 dyes per laminin heterotrimer
• <5% free dye detectable in the final product
• Supplied as a lyophilized powder

Protein purity is determined by scanning densitometry of Coomassie Blue-stained protein on a 4-20% polyacrylamide gel. LMN01 is >90% pure.

Biological activity of LMN01 is demonstrated by its ability to act as a substrate for cell invasion as monitored through an ECM degradation assay.